The DOI matches its Crossref record; 103 of the 103 references it cites were found in a registry or an index.
Thrombopoietin: the primary regulator of platelet production [see comments]
Open accessAbstract
CONCEPT was advanced some 37 years ago, evidence for the existence of a humoral regulator of platelet production, first termed thrombopoietin (Tpo) by Kelemen et al,' accumulated very slowly.Initial studies in the 1960s and 1970s indicated that thrombocytopenia evokes characteristic marrow changes including increases in the number, size, ploidy, and maturation rate of megakaryocyte^.^"Platelet transfusion-induced thrombocytosis was associated with the opposite effects.*These findings were later confirmed with the availability of improved methods of assessing cellular DNA content and megakaryocyte maturation.'"'Ode11 et allz are generally acknowledged as providing the first description of a humoral substance in the sera of thrombocytopenic rats which induced thrombocytosis in recipient animals.Subsequent investigators have reported that thrombocytopenic serum also induces many of the marrow effects associated with thromb~cytopenia.'~"~However, significant progress in the field awaited the development of reliable, albeit cumbersome, assays of Tpo activity.I7-*' Attempts to biochemically purify Tpo from plasma, serum, urine, and conditioned culture medium from various cell lines occupied much effort during the 1 9 8 0 ~.~" ~~ Unfortunately, because of the complex nature of the starting material and difficulties associated with the use of an in vivo assay, these attempts failed to yield homogeneous protein adequate for protein sequencing and cDNA cloning, the official rite of passage for a hematopoietic growth factor.Furthermore, a number of cytokines were described in the late 1980s and early 1990s that possessed activity in various assays of megakaryocyte development.2943During the past several years, many investigators suggested that a distinct, lineage-specific thrombopoietin may not exist. THE SEARCH FOR TPOFour groups have published extensively on the purification of substances that either augment platelet production or stimulate megakaryocyte development.Levin's group has reported on the biologic effects of Tpo partially purified from thrombocytopenic rabbit plasma.24b27"*45These workers used ammonium sulfate fractionation, two types of lectin affinity chromatography, and gel filtration on high-pressure liquid chromatography (HPLC) columns to obtain material that reproducibly increased platelet production in recipient animals.Its reported molecular weight (M,) was 40 to 47 kD."Additional biologic activities ascribed to partially purified Tpo include augmentation of megakaryocyte ploidy, both in vitro Blood, Voi 86, No 2 (July 15).
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Thrombopoietin activates the JAK/STAT3 pathway in megakaryocytes”
From Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 To Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution (2020) · cites this paper as Kaushansky, 1995
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kishimoto, T., Taga, T., & Akira, S. (1994) · Cell · doi:10.1016/0092-8674(94)90333-6 · Cited by 1,318
Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand
de Sauvage, F. J., Hass, P. E., Spencer, S. D., Malloy, B., Gurney, A. L., Spencer, S. A., Darbonne, W. C., Henzel, W. J., Wong, S. C., Kuang, W.-J., et al. (1994) · Nature · doi:10.1038/369533a0 · Cited by 1,270
Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo
Lok, S., Kaushansky, K., Holly, R. D., Kuijper, J. L., Lofton–Day, C., Oort, P. J., Grant, F. J., Heipel, M., Burkhead, S. K., Kramer, J. M., et al. (1994) · Nature · doi:10.1038/369565a0 · Cited by 1,068
Self-citationKaushansky, K., Lok, S., Holly, R. D., Broudy, V. C., Lin, N. L., Bailey, M. C., Forstrom, J. W., Buddle, M. M., Oort, P. J., Hagen, F. S., et al. (1994) · Nature · doi:10.1038/369568a0 · Cited by 1,024
Self-citationIkebuchi, K., Wong, G. G., Clark, S., Ihle, J. N., Hirai, Y., & Ogawa, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.24.9035 · Cited by 800
Paul, S. R., Bennett, F., Calvetti, J., Kelleher, K. S., Wood, C. R., O'Hara, R. M., Leary, A. C., Sibley, B. S., Clark, S., & Williams, D. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.19.7512 · Cited by 651
Thrombocytopenia in c-mpl-deficient mice
Gurney, A. L., Carver-Moore, K., de Sauvage, F., & Moore, M. W. (1994) · Science · doi:10.1126/science.8073287 · Cited by 648
c-Mpl ligand is a humoral regulator of megakaryocytopoiesis
Wendling, F., Maraskovsky, E., Debili, N., Florindo, C., Teepe, M., Titeux, M., Methia, N., BRETON-GORIUS, J., Cosman, D. J., & Vainchenker, W. (1994) · Nature · doi:10.1038/369571a0 · Cited by 640
HEL Cells: A New Human Erythroleukemia Cell Line with Spontaneous and Induced Globin Expression
Martin, P. J., & Papayannopoulou, T. (1982) · Science · doi:10.1126/science.6177045 · Cited by 608
Vigon, I., Mornon, J., Petit, L., Mitjavila, M. T., Tambourin, P. E., Gisselbrecht, S., & Souyri, M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5640 · Cited by 504
Research works printed but not linked: 2
- Shivdasani RA, Rosenblatt M F , Vignali K, Zucker-Franklin D, Jackson CW, Hunt P, Saris CJM, Orkin SH: Transcription factor NF-E2 is required for platelet formation independent of the actions SUPPl 1) of thrombopoietin/MGDF in megakaryocyte development. Cell (in press) 106.
- Geissler K, Stockenhuber F, Hinterberger W, Balcke B, Lechner K Recombinant human erythropoietin: A multipotential hemopoietic growth factor in vivo and in vitro, in Schaefer RM, Heidland A, Horl WH (eds): Erythropoietin in the 90s. Contributions to Nephrology, v01 87. Basel, Switzerland, Karger, 1990, p 1 142. Moore MAS, Warren DJ: Synergy of interleukin-l and gran- ulocyte colony-stimulating factor: In vivo stimulation of stem-cell recovery and hematopoietic regeneration following 5-fluorouracil treatment of mice. Proc Natl Acad Sci USA 84:7134, 1987 143.
Cited by
Sorted by citations
Mean platelet volume as a predictor of cardiovascular risk: a systematic review and meta‐analysis
CHU, S. G., Becker, R. C., Berger, P. B., Bhatt, D. L., Eikelboom, J. W., Konkle, B. A., Mohler, E. R., Reilly, M. P., & Berger, J. S. (2009) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2009.03584.x · Cited by 1,055
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Lyman, S. D., & Jacobsen, S. E. W. (1998) · Blood · doi:10.1182/blood.v91.4.1101 · Cited by 686
Interleukin-6 stimulates thrombopoiesis through thrombopoietin: role in inflammatory thrombocytosis
Kaser, A., Brandacher, G., Steurer, W., Kaser, S., Offner, F. A., Zoller, H., Theurl, I., Widder, W., Molnar, C., Ludwiczek, O., et al. (2001) · Blood · doi:10.1182/blood.v98.9.2720 · Cited by 667
Dynamic Visualization of Thrombopoiesis Within Bone Marrow
Junt, T., Schulze, H., Zhao, C. N., Maßberg, S., Goerge, T., Krueger, A., Wagner, D. D., Graf, T., Italiano, J. E., Shivdasani, R. A., et al. (2007) · Science · doi:10.1126/science.1146304 · Cited by 646
The molecular mechanisms that control thrombopoiesis
Kaushansky, K. (2005) · Journal of Clinical Investigation · doi:10.1172/jci26674 · Cited by 561
Self-citation
Metadata
- Authors
- Kaushansky, K.
- Corresponding author
- Kaushansky, K
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1995-07-15
- Volume
- 86
- Issue
- 2
- Pages
- 419-431
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2230842386
- Cited by
- 642
- References
- 103
- Citations by year
- 2026: 6 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 7 · 2020: 5 · 2019: 6 · 2018: 5 · 2017: 13 · 2016: 13 · 2015: 8 · 2014: 15 · 2013: 5 · 2012: 10
- FWCI
- 39.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Health Sciences › Medicine › Hematology › Myeloproliferative Neoplasms: Diagnosis and Treatment
- Keywords
- thrombopoietin, megakaryopoiesis, megakaryocyte development, thrombocytopenia, thrombocytosis, hematopoietic growth factors, thrombopoietin receptor, cDNA cloning, protein purification, cytokines, megakaryocyte maturation, platelet transfusion
- MeSH terms
- Animals, Blood Platelets, Cell Division, Gene Expression Regulation, Genes, Hematopoiesis, Humans, Megakaryocytes, Mice, Inbred BALB C, Radiation Injuries, Experimental, Receptors, Immunologic, Thrombocytopenia, Thrombopoietin, Carboplatin, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
