Detection of major bcr-abl gene expression at a very low level in blood cells of some healthy individuals
Open accessAbstract
The major bcr-abl fusion gene is presently seen as the hallmark of chronic myeloid leukemia (CML) and presumably as the cause of its development. Accordingly, long-term disappearance of bcr-abl after intensive therapy is considered to be a probable cure of CML. The nested reverse transcriptase-polymerase chain reaction (RT-PCR) provides a powerful tool for minimal residual CML detection. The RT-PCR was optimized by (1) increasing the amount of total RNA involved in the reverse transcription reaction to correspond to total RNA extracted from 10(8) cells, (2) using a specific abl primer in this reverse reaction, and (3) reamplifying 10% of the RT-PCR product in nested amplification. This optimized RT-PCR permitted us to detect up to 1 copy of RNA bcr-abl synthesised in vitro, mixed with yeast RNA in an equivalent quantity to 10(8) white blood cells (WBCs). Using this highly sensitive RT-PCR during the follow-up of CML patients, a signal was unexpectedly found in healthy controls. Therefore, a systematic study of the possible expression of bcr-abl RNA in the WBCs of healthy adults and children and in umbilical cord blood was undertaken. It showed the presence of bcr-abl transcript in the blood of 22 of 73 healthy adults and in the blood of 1 of 22 children but not in 22 samples of umbilical cord blood.
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Previously described mutations in the blood of healthy persons such as BCR-ABL or BCL2 translocations were usually present in less than 0.01% of cells and were often transient.
“For comparison, previously described mutations in the blood of healthy persons such as BCR-ABL or BCL2 translocations were usually present in less than 0.01% of cells and were often transient 29,30.”
From Clonal hematopoiesis in human aging and disease (2019) · cites this paper as [29]
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- Authors
- Biernaux, C., Loos, M., Sels, A., Huez, G., & Stryckmans, P.
- Affiliations
- Université Libre de Bruxelles (BE)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1995-10-15
- Volume
- 86
- Issue
- 8
- Pages
- 3118-3122
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1599349966
- Cited by
- 485
- References
- 34
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 9 · 2023: 15 · 2022: 11 · 2021: 12 · 2020: 15 · 2019: 10 · 2018: 11 · 2017: 10 · 2016: 19 · 2015: 17 · 2014: 16 · 2013: 8 · 2012: 14
- FWCI
- 10.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Chronic Myeloid Leukemia Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Keywords
- BCR-ABL fusion gene, chronic myeloid leukemia, minimal residual disease, healthy individuals, leukocytes, umbilical cord blood, children
- MeSH terms
- Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Base Sequence, Child, Child, Preschool, Fetal Blood, Gene Expression Regulation, Humans, Infant, Leukocytes, Middle Aged, Molecular Sequence Data, Reference Values, Sensitivity and Specificity, Fusion Proteins, bcr-abl, Polymerase Chain Reaction, Genes, abl
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