STAT-related transcription factors are constitutively activated in peripheral blood cells from acute leukemia patients
Open accessAbstract
A signal transduction pathway activated by many cytokines has recently been elaborated. The JAK kinases and the signal transducers and activators of transcription (STAT) factors have been found to be essential components. In this report, we describe the presence of constitutively activated STAT factors in peripheral blood cells from patients with acute leukemia. We used oligonucleotide probes from the beta-casein and IRF-1 gene promoters and the ISRE probe to detect STAT proteins in nuclear extracts from acute leukemia cells in bandshift assays. Specific DNA protein complex formation was observed with the probes from the beta-casein and IRF-1 gene promoters, but not with the ISRE oligonucleotide probe, when cell extracts from acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) were investigated. We used nonradioactive oligonucleotides as competitors to show the specificity of the complex formation. Specific antibodies directed against the individual STAT proteins were used in supershift experiments. STAT5- and STAT1-related factors were detected in ALL and STAT1-, STAT3-, and STAT5-related proteins were present in nuclear cell extracts from AML. Since the cells were not treated with cytokines before the nuclear proteins were extracted, we conclude that these factors are constitutively activated in vivo. It is likely that the constitutive activation of STAT proteins is a part of the events of leukemogenesis.
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A number of transformed cell lines as well as samples from human cancers are reported to contain constitutively activated STAT3.
“A number of transformed cell lines as well as samples from human cancers are reported to contain constitutively activated STAT3.”
From STAT3 Contributes to the Mitogenic Response of Hepatocytes during Liver Regeneration (2002) · cites this paper as [25]
The Results report constitutive DNA-binding by a STAT3-related factor in several patients’ AML samples, supporting the human-cancer-sample part of the claim. The Discussion also cites STAT3-related activity in Src-transformed cells, but it does not establish constitutively activated STAT3 in a number of transformed cell lines; it notes that the factor detected in AML may differ from STAT3. The indexed passages provide no page numbers. (LLM, conf=0.84)
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- Authors
- Gouilleux-Gruart, V, Gouilleux, F., Desaint, C., Claisse, J., Capiod, J., Delobel, J., Weber-Nordt, R., Dusanter-Fourt, I., Dreyfus, F., Groner, B., & Prin, L.
- Affiliations
- Laboratoire d'Immunologie, Centre Hospitalier Universitaire d'Amiens, France.
- Centre Hospitalier Universitaire Amiens-Picardie (FR)
- ORCID
- Gouilleux, F
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1996-03-01
- Volume
- 87
- Issue
- 5
- Pages
- 1692-1697
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W159302622
- Cited by
- 316
- References
- 0
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 7 · 2014: 11 · 2013: 6 · 2012: 12
- FWCI
- 8.52
- Type
- journal article
- Language
- en
- Open access
- green
- hal.science
- Licence
- other-oa
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Keywords
- STAT transcription factors, constitutive activation, acute leukemia, acute lymphoblastic leukemia, acute myeloid leukemia, JAK/STAT signaling, beta-casein promoter, interferon-stimulated response element, STAT1, STAT3, STAT5, leukemogenesis, peripheral blood cells
- MeSH terms
- Acute Disease, Adult, Aged, Base Sequence, Blood Cells, DNA-Binding Proteins, DNA, Neoplasm, Humans, Leukemia, Milk Proteins, Molecular Sequence Data, Neoplasm Proteins, Promoter Regions, Genetic, Transcription, Genetic, Neoplastic Stem Cells, Signal Transduction, Trans-Activators, Gene Expression Regulation, Leukemic, Macromolecular Substances, STAT1 Transcription Factor, STAT3 Transcription Factor, STAT5 Transcription Factor
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