CoChat
Sign Up
Cited by 151

The DOI matches its Crossref record.

Multiple inhibitory cytokines induce deregulated progenitor growth and apoptosis in hematopoietic cells from Fac-/- mice

Haneline, Laura S., Broxmeyer, Hal E., Cooper, Scott, Hangoc, Giao, Carreau, Madeleine, Buchwald, Manuel, Clapp, D. Wade·1998·Blood
DOI 10.1182/blood.v91.11.4092
Open this paper in CoChat — free account

Abstract

We used a murine model containing a disruption of the murine homologue (Fac) of Fanconi Anemia group C (FAC) to evaluate the role of Fac in the pathogenesis of bone marrow (BM) failure. Methylcellulose cultures of BM cells fromFac−/− and Fac+/+ mice were established to examine the growth of multipotent and lineage-restricted progenitors containing inhibitory cytokines, including interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and macrophage inflammatory protein-1α (MIP-1α). Clonogenic growth of Fac−/− progenitors was reduced by 50% at 50- to 100-fold lower concentrations of all inhibitory cytokines evaluated. We hypothesized that the aberrant responsiveness to inhibitory cytokines in clonogenic cells may be a result of deregulated apoptosis. To test this hypothesis, we performed the TUNEL assay on purified populations of primary BM cells enriched for hematopoietic progenitors or differentiated myeloid cells. After stimulation with TNF-α, accentuated apoptosis was observed in both populations of Fac−/− cells. In addition, deregulated apoptosis was also noted in the most immature phenotypic population of hematopoietic cells after stimulation with MIP-1α.Together these data suggest a role of Fac in affecting the signaling of multiple cytokine pathways and support cytokine-mediated apoptosis as a major mechanism responsible for BM failure observed in FA patients.

Overview

Integrity signals

Registry

Crossref · 10.1182/blood.v91.11.4092

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 50

  1. Auerbach, 1992, Fanconi anemia and leukemia: Tracking the genes., Leukemia, 6, 1
  2. Auerbach, 1993, Fanconi anemia diagnosis and the diepoxybutane (DEB) test., Exp Hematol, 21, 731
  3. Buchwald, 1987, Studies of gene transfer and reversion to mitomycin C resistance in Fanconi anemia cells., Mutat Res, 184, 153
  4. Schroeder, 1976, Formal genetics of Fanconi's anemia., Hum Genet, 32, 257, 10.1007/BF00295817 · DOI
  5. Strathdee, 1992, Evidence for at least four Fanconi Anemia genes including FACC on chromosome 9., Nature Genet, 1, 196, 10.1038/ng0692-196 · DOI
  6. Joenje, 1997, Evidence for at least eight Fanconi anemia genes., Am J Hum Genet, 61, 940, 10.1086/514881 · DOI
  7. Pronk, 1995, Localization of the Fanconi anemia complementation group A gene to chromosome 16q24.3., Nature Genet, 11, 338, 10.1038/ng1195-338 · DOI
  8. Whitney, 1995, Microcell mediated chromosome transfer maps the Fanconi anemia groups D gene to chromosome 3p., Nature Genet, 11, 341, 10.1038/ng1195-341 · DOI
  9. Strathdee, 1992, Cloning of cDNAs for Fanconi's anaemia by functional complementation., Nature, 356, 763, 10.1038/356763a0 · DOI
  10. Lo Ten Foe, 1996, Expression cloning of a cDNA for the major Fanconi anaemia gene, FAA., Nature Genet, 14, 320, 10.1038/ng1196-320 · DOI
  11. The Fanconi anaemia/breast cancer consortium, 1996, Positional cloning of the Fanconi anaemia group A gene., Nature Genet, 14, 324, 10.1038/ng1196-324 · DOI
  12. Broxmeyer, 1992, Suppressor cytokines and regulation of myelopoiesis: Biology and possible clinical uses., Am J Pediatr, 14, 22
  13. Broxmeyer, 1996, Myelosuppressive cytokines and peptides, 121
  14. Chen, 1996, Inactivation of Fac in mice produces inducible chromosomal instability and reduced fertility reminiscent of Fanconi anaemia., Nature Genet, 12, 448, 10.1038/ng0496-448 · DOI
  15. Whitney, 1996, Germ cell defects and hematopoietic hypersensitivity to γ-interferon in mice with a targeted disruption of the Fanconi anemia C gene., Blood, 88, 49, 10.1182/blood.V88.1.49.49 · DOI · Paper page
  16. Rathbun, 1997, Inactivation of the Fanconi Anemia Group C gene augments interferon-γ–induced apoptotic responses in hematopoietic cells., Blood, 90, 974, 10.1182/blood.V90.3.974 · DOI · Paper page
  17. Maciejewsk, 1995, Fas antigen expression on CD34+ human marrow cells is induced by interferon γ and tumor necrosis factor α and potentiates cytokine-mediated hematopoietic suppression in vitro., Blood, 85, 3183, 10.1182/blood.V85.11.3183.bloodjournal85113183 · DOI
  18. Schultz, 1993, Tumor necrosis factor-alpha overproduction in Fanconi's anemia., Am J Hematol, 42, 196, 10.1002/ajh.2830420211 · DOI
  19. Rosselli, 1994, Abnormal lymphokine production: A novel feature of the genetic disease Fanconi anemia. II. In vitro and in vivo spontaneous overproduction of tumor necrosis factor alpha., Blood, 83, 1216, 10.1182/blood.V83.5.1216.1216 · DOI
  20. Liu, 1996, Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-κB activation prevents cell death., Cell, 87, 565, 10.1016/S0092-8674(00)81375-6 · DOI · Paper page
  21. Broxmeyer, 1988, Growth characteristics of marrow hematopoietic progenitor/precursor cells from patients on a phase I clinical trial with purified recombinant human granulocyte-macrophage colony-stimulating factor., Exp Hematol, 16, 594
  22. Broxmeyer, 1995, Effects of in vivo treatment with PIXY321 (GM-CSF/IL3 fusion protein) on proliferation kinetics of bone marrow and blood myeloid progenitor cells in patients with sarcoma., Exp Hematol, 23, 335
  23. Broxmeyer, 1976, Relationship of colony stimulating activity to apparent kill of human colony forming cells by irradiation and hydroxyurea., Blood, 47, 403, 10.1182/blood.V47.3.403.403 · DOI
  24. Negoescu, 1997, TUNEL: Improvement and evaluation of the method for in situ apoptotic cell identification., Biochemica, 2, 12
  25. Broxmeyer, 1993, Comparative analysis of the influences of human gamma, alpha and beta interferons on human multipotential (CFU-GEMM), erythroid (BFU-E), and granulocyte-macrophage (CFU-GM) progenitor cells., J Immunol, 131, 1300, 10.4049/jimmunol.131.3.1300 · DOI
  26. Broxmeyer, 1986, The suppressive influences of human tumor necrosis factor on bone marrow hematopoietic cells from donors and patients with leukemia: Synergism of tumor necrosis factor and gamma interferon., J Immunol, 136, 4487, 10.4049/jimmunol.136.12.4487 · DOI
  27. Graham, 1990, Identification and characterization of an inhibitor of haemopoietic stem cell proliferation., Nature, 344, 442, 10.1038/344442a0 · DOI
  28. Broxmeyer, 1990, Enhancing and suppressing effects of recombinant murine macrophage inflammatory proteins on colony formation in vitro by bone marrow myeloid progenitor cells., Blood, 76, 1110, 10.1182/blood.V76.6.1110.1110 · DOI
  29. Cooper, 1994, Myelosuppressive effects in vivo with very low dosages of monomeric recombinant murine macrophage inflammatory protein-1α., Exp Hematol, 22, 186
  30. Sakai, 1997, The kinase domain of Jak2 mediates induction of bcl-2 and delays cell death in hematopoietic cells., J Biol Chem, 272, 12350, 10.1074/jbc.272.19.12350 · DOI
  31. Lotem, 1992, Hematopoietic cytokines inhibit apoptosis induced by transforming growth factor beta 1 and cancer chemotherapy compounds in myeloid leukemic cells., Blood, 80, 1750, 10.1182/blood.V80.7.1750.bloodjournal8071750 · DOI
  32. Wynshaw-Boris, 1996, Model mice and human disease., Nature Genet, 13, 259, 10.1038/ng0796-259 · DOI
  33. Young, 1993, The bone marrow failure syndromes, 216
  34. Butturini, 1994, Hematologic abnormalities in Fanconi anemia. An International Fanconi Anemia Registry study., Blood, 84, 1650, 10.1182/blood.V84.5.1650.1650 · DOI · Paper page
  35. D'Andrea, 1997, Molecular biology of Fanconi anemia: Implications for diagnosis and therapy., Blood, 90, 1725, 10.1182/blood.V90.5.1725 · DOI
  36. Lambert, 1997, Correction of the FNA repair defect in Fanconi anemia complementation groups A and D cells., Biochem Biophys Res Commun, 230, 587, 10.1006/bbrc.1996.6008 · DOI
  37. Joenje, 1983, Effect of oxygen tension on chromosomal aberrations in Fanconi anaemia., Hum Genet, 65, 99, 10.1007/BF00286642 · DOI
  38. Clarke, 1997, The sensitivity of Fanconi anaemia group C cells to apoptosis induced by mitomycin C is due to oxygen radical generation, not DNA crosslinking., Br J Haematol, 96, 240, 10.1046/j.1365-2141.1997.d01-2023.x · DOI
  39. Ruppitsch, 1997, The role of oxygen metabolism for the pathological phenotype of Fanconi anemia., Hum Genet, 99, 710, 10.1007/s004390050437 · DOI
  40. Cumming, 1996, Suppression of apoptosis in hematopoietic factor-dependent progenitor cell lines by expression of the FAC gene., Blood, 88, 4558, 10.1182/blood.V88.12.4558.bloodjournal88124558 · DOI
  41. Ridet, 1997, Deregulated apoptosis is a hallmark of the Fanconi anemia syndrome., Cancer Res, 57, 1722
  42. Selleri, 1996, Interferon-gamma constituitively expressed in the stromal microenvironment of human marrow cultures mediates potent hematopoietic inhibition., Blood, 87, 4149, 10.1182/blood.V87.10.4149.bloodjournal87104149 · DOI · Paper page
  43. Young, 1997, The pathophysiology of acquired aplastic anemia., N Engl J Med, 336, 1365, 10.1056/NEJM199705083361906 · DOI
  44. Aronica, 1995, Interferon-inducible protein 10 and macrophage inflammatory protein 1α inhibit growth factor stimulation of Raf-1 kinase activity and protein synthesis in a human growth factor-dependent hematopoietic cell line., J Biol Chem, 270, 21998, 10.1074/jbc.270.37.21998 · DOI
  45. Selleri, 1995, Interferon-gamma and tumor necrosis factor-alpha suppress both early and late stages of hematopoiesis and induce programmed cell death., J Cell Physiol, 165, 538, 10.1002/jcp.1041650312 · DOI
  46. Suzuki, 1988, Effects of human interferon on cellular response to UV in UV-sensitive human cell strains., Mutat Res, 198, 207, 10.1016/0027-5107(88)90056-5 · DOI
  47. Gaspari, 1993, Impaired interferon production and natural killer cell activation in patients with the skin cancer-prone disorder, xeroderma pigmentosum., J Clin Invest, 92, 1135, 10.1172/JCI116682 · DOI
  48. Siddoo-Atwal, 1996, Elevation of interferon β-inducible proteins in ataxia telangiectasia cells., Cancer Res, 56, 443
  49. Auerbach, 1997, Disorders of DNA replication and repair., Curr Opin Pediatr, 9, 599, 10.1097/00008480-199712000-00010 · DOI
  50. Heinrich, 1998, DNA cross-linker-induced G2/M arrest in group C Fanconi anemia lymophoblasts reflects normal checkpoint function., Blood, 91, 275, 10.1182/blood.V91.1.275 · DOI

Cited by

See all 151

Metadata

Authors
Haneline, L. S., Broxmeyer, H. E., Cooper, S., Hangoc, G., Carreau, M., Buchwald, M., & Clapp, D. W.
Affiliations
1 From the Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine; the Department of Microbiology/Immunology, Indiana University School of Medicine; Walther Oncology Center, Indiana University School of Medicine, Indianapolis; Walther Cancer Institute, Indianapolis, IN; the Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada; and the Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada.
Venue
Blood
Publisher
American Society of Hematology
Published
1998-06-01
Volume
91
Issue
11
Pages
4092-4098
ISSN
  • 1528-0020
  • 0006-4971
DOI
10.1182/blood.v91.11.4092
Cited by
151
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Multiple inhibitory cytokines induce deregulated progenitor growth and apoptosis in hematopoietic cells from Fac-/- mice. https://app.cochat.ai/papers/10.1182/blood.v91.11.4092
The paper
Haneline, L. S., Broxmeyer, H. E., Cooper, S., Hangoc, G., Carreau, M., Buchwald, M., & Clapp, D. W. (1998). Multiple inhibitory cytokines induce deregulated progenitor growth and apoptosis in hematopoietic cells from Fac-/- mice. Blood. https://doi.org/10.1182/blood.v91.11.4092