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Cited by 183Hematopoietic Stem Cell TransplantationT-cell and B-cell ImmunologyMesenchymal stem cell research

Lymphoid reconstitution after autologous PBSC transplantation with FACS-sorted CD34+ hematopoietic progenitors.

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Bomberger, Catherine, Singh-Jairam, Meeta, Rodey, Glenn, Guerriero, Anastasia, Yeager, Andrew M., Fleming, William H., Holland, H. Kent, Waller, Edmund K.·1998·Blood
DOI 10.1182/blood.v91.7.2588
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Abstract

T-cell and B-cell reconstitution was studied in nine patients who received fluorescence activated cell sorter (FACS)-sorted autologous CD34+ hematopoietic progenitor cells (HPC). The mean numbers of T cells (CD3+), B cells (CD19+) and CD34+ HPC administered to each patient were .004, .002, and 1.8 × 106 cells/kg, respectively. After high-dose myeloablative chemotherapy (busulfan, cyclophosphamide, etoposide) CD34+ HPC were infused and lymphoid reconstitution was monitored using flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of VDJ T-cell receptor (TcR) sequences. Restoration of normal numbers of peripheral blood T cells and B cells among recipients of FACS-sorted CD34+ HPC was delayed compared to recipients of non-T-cell–depleted PBSC autografts. In both patient groups, the circulating T cells were primarily CD4−, CD8+, αβ TcR+, and CD45RO+, CD45RA− during the first 2 months after transplant. Subsequent increases in the frequency of CD45RA+ CD45RO− T cells occurred at 2 to 3 months after transplant, suggesting maturation of CD34+hematopoietic progenitors to “naive” T cells. Analysis of the TcR repertoire after hematopoietic reconstitution demonstrated decreased diversity of Vβ TcR expression associated with global decreases in the absolute number of total peripheral blood T cells and most Vβ TcR+ subsets. Three of nine recipients of FACS-sorted CD34+ HPC demonstrated significant increases in the percentage of γδ+ peripheral T cells and CD5+ B cells at 3 to 9 weeks after transplantation, and all patients had transient oligoclonal expansions of T cells expressing specific Vβ TcR. Transplantation with highly purified CD34+ HPC results in reduced diversity of the peripheral T-cell repertoire during the early post-transplant period compared with patients receiving unmanipulated or MoAb-depleted transplants.

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  • CTX001 may have contributed to delayed lymphocyte recovery, similar to T-cell-depleted transplantation.

    “As a product that is enriched with CD34+ cells, CTX001 may have contributed to the delay in lymphocyte recovery, similar to what has been observed after T-cell-depleted transplantation.33,34”

    From CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia (2021) · cites this paper as [33]

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Research works printed but not linked: 1

  1. [8]8. Jacobs C, Shpall E, Ball E, Champlin R, LeMaistre C, Holland H, Saral R, Berenson R: A prospective randomized phase III study using the CEPRATE SC stem cell concentrator to isolate CD34 ϩ hematopoi- etic progenitors for autologous transplantation after high dose chemo- therapy. Autologous Marrow and Blood Transplantation, Proceedings of the Seventh International Symposium 1994, p 669

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Authors
Bomberger, C., Singh-Jairam, M., Rodey, G., Guerriero, A., Yeager, A. M., Fleming, W. H., Holland, H. K., & Waller, E. K.
Affiliations
  • Emory University (US)
  • From the Stem Cell Biology Program, Emory University, Department of Pathology, Emory University, Division of Hematology and Oncology, Atlanta, GA.
ORCID
Venue
Blood
Publisher
American Society of Hematology
Published
1998-04-01
Volume
91
Issue
7
Pages
2588-2600
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood.v91.7.2588
OpenAlex
W2407278011
Cited by
183
References
36
Citations by year
2026: 1 · 2024: 5 · 2022: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 7 · 2012: 10
FWCI
12.33
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
lymphoid reconstitution, autologous stem cell transplantation, CD34+ hematopoietic progenitor cells, T cell reconstitution, B cell reconstitution, T cell receptor repertoire, naive T cells, γδ T cells, CD5+ B cells, oligoclonal expansion, myeloablative chemotherapy, T cell depletion
MeSH terms
Adult, B-Lymphocytes, Cell Differentiation, Cell Separation, Flow Cytometry, Graft Survival, Humans, Lymphoma, Non-Hodgkin, Middle Aged, T-Lymphocytes, Transplantation, Autologous, Hematopoietic Stem Cell Transplantation, Antigens, CD34

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