The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Involvement of Caspases in Neutrophil Apoptosis: Regulation by Reactive Oxygen Species
Abstract
Human neutrophils have a short half-life and are believed to die by apoptosis or programmed cell death both in vivo and in vitro. We found that caspases are activated in a time-dependent manner in neutrophils undergoing spontaneous apoptosis, concomitant with other characteristic features of apoptotic cell death such as morphologic changes, phosphatidylserine (PS) exposure, and DNA fragmentation. The treatment of neutrophils with agonistic anti-Fas monoclonal antibodies (MoAbs) significantly accelerated this process. However, in cells treated with the potent neutrophil activator phorbol 12-myristate 13-acetate (PMA), caspase activity was only evident after pharmacologic inhibition of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Similarily, inhibition of the NADPH oxidase in constitutive and Fas/APO-1–triggered apoptosis resulted in increased rather than suppressed levels of caspase activity, suggesting that reactive oxygen species may prevent caspases from functioning optimally in these cells. Moreover, oxidants generated via the NADPH oxidase were essential for PS exposure during PMA-induced cell death, but not for neutrophils undergoing spontaneous apoptosis. We conclude that caspases are an important component of constitutive and Fas/APO-1–triggered neutrophil apoptosis. However, these redox sensitive enzymes are suppressed in activated neutrophils, and an alternate oxidant-dependent pathway is used to mediate PS exposure and neutrophil clearance under these conditions.
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Cites
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Cited by
Neutrophil Extracellular Traps Kill Bacteria
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y., & Zychlinsky, A. (2004) · Science · doi:10.1126/science.1092385 · Cited by 10,268
Novel cell death program leads to neutrophil extracellular traps
Fuchs, T. A., Abed, U., Goosmann, C., Hurwitz, R., Schulze, I., Wahn, V., Weinrauch, Y., Brinkmann, V., & Zychlinsky, A. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200606027 · Cited by 3,197
Carr, A., & Maggini, S. (2017) · Nutrients · doi:10.3390/nu9111211 · Cited by 2,035
Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity
Bogdan, C., Röllinghoff, M., & Diefenbach, A. (2000) · Current Opinion in Immunology · doi:10.1016/s0952-7915(99)00052-7 · Cited by 939
Neutrophil extracellular trap cell death requires both autophagy and superoxide generation
Remijsen, Q., Berghe, T. V., Wirawan, E., Asselbergh, B., Parthoens, E., De Rycke, R., Noppen, S., Delforge, M., Willems, J., & Vandenabeele, P. (2011) · Cell Research · doi:10.1038/cr.2010.150 · Cited by 818
Metadata
- Authors
- Fadeel, B., Åhlin, A., Henter, J.-I., Orrenius, S., & Hampton, M. B.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1998-12-15
- Volume
- 92
- Issue
- 12
- Pages
- 4808-4818
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1032376300
- Cited by
- 347
- References
- 45
- FWCI
- 4.95
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fadeel, B., Åhlin, A., Henter, J.-I., Orrenius, S., & Hampton, M. B. (1998). Involvement of Caspases in Neutrophil Apoptosis: Regulation by Reactive Oxygen Species. Blood. https://doi.org/10.1182/blood.v92.12.4808
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
