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The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum
Open accessAbstract
The relative importance of host and bacteria-derived deoxyribonucleic acid (DNA) in the increased viscoelasticity of purulent sputum in cystic fibrosis (CF) and other airway diseases is unclear. We report the identification of the DNA associated with mucus glycoproteins purified from the purulent sputum of 9 patients with CF. Mucus glycoproteins were purified from CF sputum by gel exclusion chromatography and the co-purifying DNA isolated by phenol extraction. Electrophoresis indicated that the DNA preparations had a size of approximately 300 to greater than 50,000 bases. The origin of the DNA was determined by slot blotting and subsequent hybridization with 32P-labelled DNA probes specific for human DNA sequences and those from bacterial species commonly isolated from CF sputum. The results indicated that in all cases the DNA was almost entirely human in origin. This implies that it is the patient's own DNA which may contribute to the rheological abnormalities of CF sputum.
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Cited by
Vandivier, R. W., Fadok, V. A., Hoffmann, P. R., Bratton, D. L., Penvari, C., Brown, K. K., Brain, J. D., Accurso, F. J., & Henson, P. M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci13572 · Cited by 373
Enhanced Pseudomonas aeruginosa Biofilm Development Mediated by Human Neutrophils
Walker, T. S., Tomlin, K. L., Worthen, G. S., Poch, K. R., Lieber, J. G., Saavedra, M. T., Fessler, M. B., Malcolm, K. C., Vasil, M. L., & Nick, J. A. (2005) · Infection and Immunity · doi:10.1128/iai.73.6.3693-3701.2005 · Cited by 263
Bikel, S., Valdez-Lara, A., Cornejo-Granados, F., Rico, K., Canizales-Quinteros, S., Soberón, X., Del Pozo-Yauner, L., & Ochoa-Leyva, A. (2015) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2015.06.001 · Cited by 263
Caceres, S. M., Malcolm, K. C., Taylor-Cousar, J. L., Nichols, D. P., Saavedra, M. T., Bratton, D. L., Moskowitz, S. M., Burns, J. L., & Nick, J. A. (2014) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.03514-14 · Cited by 50
Metadata
- Authors
- Lethem, M., James, S., Marriott, C., & Burke, J.
- Venue
- European Respiratory Journal
- Publisher
- European Respiratory Society (ERS)
- Published
- 1990-01-01
- Volume
- 3
- Issue
- 1
- Pages
- 19-23
- ISSN
- 0903-1936 (ISSN-L)
- 1399-3003
- OpenAlex
- W2156361445
- Cited by
- 215
- References
- 0
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum. https://app.cochat.ai/papers/10.1183/09031936.93.03010019
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- Lethem, M., James, S., Marriott, C., & Burke, J. (1990). The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum. European Respiratory Journal. https://doi.org/10.1183/09031936.93.03010019
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
