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BISMA - Fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences

Open access
Rohde, Christian, Zhang, Yingying, Reinhardt, Richard, Jeltsch, Albert·2010·BMC Bioinformatics

Abstract

Background: Bisulfite sequencing is a popular method to analyze DNA methylation patterns at high resolution. A region of interest is targeted by PCR and about 20-50 subcloned DNA molecules are usually analyzed, to determine the methylation status at single CpG sites and molecule resolution. Results: The BISMA (Bisulfite Sequencing DNA Methylation Analysis) software for analysis of primary bisulfite sequencing data implements sequencing data extraction and enhanced data processing, quality controls, analysis and presentation of the methylation state. It uses an improved strategy for detection of clonal molecules and accurate CpG site detection and it supports for the first time analysis of repetitive sequences. Conclusions: BISMA works highly automated but still provides the user full control over all steps of the analysis. The BISMA software is freely available as an online tool for academic purposes for the analysis of bisulfite sequencing data from both unique and repetitive sequences http://biochem.jacobs-university.de/BDPC/BISMA/.

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Metadata

Authors
Rohde, C., Zhang, Y., Reinhardt, R., & Jeltsch, A.
Venue
BMC Bioinformatics
Published
2010-05-06
Volume
11
Issue
1
Pages
230-230
ISSN
1471-2105 (ISSN-L)
OpenAlex
W2051568429
Cited by
238
References
5
FWCI
6.50
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: BISMA - Fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences. https://app.cochat.ai/papers/10.1186/1471-2105-11-230
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Rohde, C., Zhang, Y., Reinhardt, R., & Jeltsch, A. (2010). BISMA - Fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences. BMC Bioinformatics. https://doi.org/10.1186/1471-2105-11-230

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