The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
IGF-1 enhances cell proliferation and survival during early differentiation of mesenchymal stem cells to neural progenitor-like cells
Open accessAbstract
BACKGROUND: There has been increasing interest recently in the plasticity of mesenchymal stem cells (MSCs) and their potential to differentiate into neural lineages. To unravel the roles and effects of different growth factors in the differentiation of MSCs into neural lineages, we have differentiated MSCs into neural lineages using different combinations of growth factors. Based on previous studies of the roles of insulin-like growth factor 1 (IGF-1) in neural stem cell isolation in the laboratory, we hypothesized that IGF-1 can enhance proliferation and reduce apoptosis in neural progenitor-like cells (NPCs) during differentiation of MSCs into NCPs.We induced MSCs differentiation under four different combinations of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, (C) EGF + bFGF + LIF, (D) EGF + bFGF + BDNF, and (E) without growth factors, as a negative control. The neurospheres formed were characterized by immunofluorescence staining against nestin, and the expression was measured by flow cytometry. Cell proliferation and apoptosis were also studied by MTS and Annexin V assay, respectively, at three different time intervals (24 hr, 3 days, and 5 days). The neurospheres formed in the four groups were then terminally differentiated into neuron and glial cells. RESULTS: The four derived NPCs showed a significantly higher expression of nestin than was shown by the negative control. Among the groups treated with growth factors, NPCs treated with IGF-1 showed the highest expression of nestin. Furthermore, NPCs derived using IGF-1 exhibited the highest cell proliferation and cell survival among the treated groups. The NPCs derived from IGF-1 treatment also resulted in a better yield after the terminal differentiation into neurons and glial cells than that of the other treated groups. CONCLUSIONS: Our results suggested that IGF-1 has a crucial role in the differentiation of MSCs into neuronal lineage by enhancing the proliferation and reducing the apoptosis in the NPCs. This information will be beneficial in the long run for improving both cell-based and cell-free therapy for neurodegenerative diseases.
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“There are also data that show that BDNF participates in the regulation of neuron metabolism, development, and proper functioning in cooperation with insulin-like growth factor-1 (IGF-1) [201].”
From BDNF as a Promising Therapeutic Agent in Parkinson’s Disease (2020) · cites this paper as [201]
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Mesenchymal and haematopoietic stem cells form a unique bone marrow niche
Méndez‐Ferrer, S., Michurina, T. V., Ferraro, F., Mazloom, A. R., MacArthur, B. D., Lira, S. A., Scadden, D. T., Ma’ayan, A., Enikolopov, G., & Frenette, P. S. (2010) · Nature · doi:10.1038/nature09262 · Cited by 3,381 · cited 1×
“ESCs are pluripotent cells derived from the inner cell mass of the blastocyst [4], whereas ASCs are isolated from adult tissues and defined as multipotent, since they are determined to produce cell types within their niche [5].”
BackgroundWagner, W., Wein, F., Seckinger, A., Frankhauser, M., Wirkner, U., Krause, U., Blake, J., Schwager, C., Eckstein, V., Ansorge, W. J., et al. (2005) · Experimental Hematology · doi:10.1016/j.exphem.2005.07.003 · Cited by 1,257 · cited 1×
“MSCs have also been found in most postnatal organs such as the umbilical cord, adipose tissue, peripheral blood, and amniotic fluid [9-11].”
BackgroundLee, J. S., Hong, J. M., Moon, G. J., Lee, P. H., Ahn, Y. H., & Bang, O. Y. (2010) · Stem Cells · doi:10.1002/stem.430 · Cited by 775 · cited 1×
“Autologous transplantation of MSCs in stroke patients was proven therapeutically effective [13].”
BackgroundDezawa, M., Kanno, H., Hoshino, M., Cho, H., Matsumoto, N., Itokazu, Y., Tajima, N., Yamada, H., Sawada, H., Ishikawa, H., et al. (2004) · Journal of Clinical Investigation · doi:10.1172/jci20935 · Cited by 684 · cited 1×
“has been reported that MSCs can be induced into neural lineage through several methods such as incubation with cytokines [19], co-culture with nerve cells [1,6], and transfection with known factors [20].”
Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells
Hermann, A., Gastl, R., Liebau, S., Popa, M. O., Fiedler, J., Boehm, B. O., Maisel, M., Lerche, H., Schwarz, J., Brenner, R. E., et al. (2004) · Journal of Cell Science · doi:10.1242/jcs.01307 · Cited by 449 · cited 2×
“This is consistent with the previous finding that one third of MSCs died after induction [14].”
BackgroundResultWislet‐Gendebien, S., Hans, G. A., Leprince, P., Rigo, J., Moonen, G., & Rogister, B. (2005) · Stem Cells · doi:10.1634/stemcells.2004-0149 · Cited by 427
Hsieh, J.-Y., Wang, H., Chang, S., Liao, K., Lee, I., Lin, W., Wu, C., Lin, W., & Cheng, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072604 · Cited by 324 · cited 1×
“Autologous transplantation of MSCs extracted from Wharton’s jelly has been reported to be free from immunogenic rejection [18].”
BackgroundWilkins, A., Kemp, K. C., Ginty, M., Hares, K. M., Mallam, E., & Scolding, N. J. (2009) · Stem Cell Research · doi:10.1016/j.scr.2009.02.006 · Cited by 290 · cited 1×
“However, recent studies suggested that ASCs, especially mesenchymal stem cells (MSCs) derived from bone marrow (BM), have intrinsic neurogenic potential and may be differentiated into mature cells of ectodermal origin [6-8].”
BackgroundMesenchymal stem cells: A promising candidate in regenerative medicine
Chen, Y., Shao, J., Xiang, L., Dong, X.-J., & Zhang, G. (2008) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2008.01.007 · Cited by 254 · cited 1×
“The trans-lineage differentiation capability and unique immunogenic properties of MSCs have made them a potential alternative for cellular transplantation use in regenerative medicine [12].”
BackgroundYang, C., Shih, Y., Ko, M., Hsu, S.-Y., Cheng, H., & Fu, Y.-S. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003336 · Cited by 231 · cited 1×
“MSCs may even release certain neurotrophic factors such as BDNF, nerve growth factor, and glial-derived neurotrophic factors [26,27], and it is well known that the neurotrophic factors are important for maintaining cellular survival of neuronal cells both in vitro and in vivo.”
BackgroundKoh, S., Kim, K. S., Choi, M., Jung, K. H., Park, K. S., Chai, Y. G., Roh, W., Hwang, S. J., Ko, H. J., Huh, Y., et al. (2008) · Brain Research · doi:10.1016/j.brainres.2008.06.087 · Cited by 209 · cited 1×
“MSCs may even release certain neurotrophic factors such as BDNF, nerve growth factor, and glial-derived neurotrophic factors [26,27], and it is well known that the neurotrophic factors are important for maintaining cellular survival of neuronal cells both in vitro and in vivo.”
BackgroundMairet‐Coello, G., Tury, A., & DiCicco-Bloom, E. M. (2009) · Journal of Neuroscience · doi:10.1523/jneurosci.1700-08.2009 · Cited by 170 · cited 2×
“IGF-1 has mitogenic effects by promoting G1/S cell cycle progression through Akt-dependent and tyrosine kinase– signalling pathways [23-25].”
BackgroundKucia, M., Zhang, Y. P., Reca, R., Wysoczynski, M., Machaliński, B., Majka, M., Ildstad, S. T., Ratajczak, J., Shields, C. B., & Ratajczak, M. Z. (2005) · Leukemia · doi:10.1038/sj.leu.2404011 · Cited by 138
Hermann, A., Liebau, S., Gastl, R., Fickert, S., Habisch, H., Fiedler, J., Schwarz, J., Brenner, R. E., & Storch, A. (2006) · Journal of Neuroscience Research · doi:10.1002/jnr.20840 · Cited by 121
Tondreau, T., Dejeneffe, M., Meuleman, N., Stamatopoulos, B., Delforge, A., Martiat, P., Bron, D., & Lagneaux, L. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-166 · Cited by 101 · cited 2×
“The antibodies used and dilution were as follows: Anti-CD90 (FITC) conjugated mouse monoclonal antibody (1:200; Thermo Scientific, USA); AntiCD44 (PE) conjugated rat monoclonal antibody (1:200; Abcam, Cambridge, UK) [17]; Alexa Fluor® 647 mouse anti-nestin (1:200; BD Pharmingen, USA); Milli-Mark™ FluoroPan neuronal ma…”
MethodCroft, A. P., & Przyborski, S. A. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0609 · Cited by 89
Egea, V., von Baumgarten, L., Schichor, C., Berninger, B., Popp, T., Neth, P., Goldbrunner, R., Kienast, Y., Winkler, F., Jochum, M., et al. (2010) · Cell Death and Differentiation · doi:10.1038/cdd.2010.154 · Cited by 84 · cited 1×
“has been reported that MSCs can be induced into neural lineage through several methods such as incubation with cytokines [19], co-culture with nerve cells [1,6], and transfection with known factors [20].”
Zhang, J., Chan, Y.-C., Ho, J. C.-Y., Siu, C., Lian, Q., & Tse, H. (2012) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00326.2011 · Cited by 82
Borowiec, A., Hague, F., Harir, N., Guénin, S., Guérineau, F., Gouilleux, F., Roudbaraki, M. M., Lassoued, K., & Ouadid‐Ahidouch, H. (2007) · Journal of Cellular Physiology · doi:10.1002/jcp.21065 · Cited by 71 · cited 2×
“IGF-1 has mitogenic effects by promoting G1/S cell cycle progression through Akt-dependent and tyrosine kinase– signalling pathways [23-25].”
BackgroundChang, S.-J., Weng, S.-L., Hsieh, J.-Y., Wang, T.-Y., Chang, M., & Wang, H.-W. (2011) · BMC Medical Genomics · doi:10.1186/1755-8794-4-65 · Cited by 68
Croft, A. P., & Przyborski, S. A. (2008) · Experimental Neurology · doi:10.1016/j.expneurol.2008.12.010 · Cited by 64 · cited 2×
“has been reported that MSCs can be induced into neural lineage through several methods such as incubation with cytokines [19], co-culture with nerve cells [1,6], and transfection with known factors [20].”
BackgroundSupeno, N. E., Pati, S., Hadi, R. A., Ghani, A. R. I., Mustafa, M. Z., Abdullah, J. M., Idris, F. M., Han, X., & Jaafar, H. (2013) · International Journal of Medical Sciences · doi:10.7150/ijms.5325 · Cited by 57 · cited 4×
“The phenotype and morphology of MSC-derived neurospheres in our study are similar to that of embryonic striatal stem cells isolated previously in the laboratory [16].”
BackgroundResultInfluentialSelf-citationA simple and efficient method for deriving neurospheres from bone marrow stromal cells
Yang, Q., Mu, J., Li, Q., Li, A., Zeng, Z., Yang, J., Zhang, X., Tang, J. B., & Xie, P. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.05.039 · Cited by 44 · cited 2×
“However, recent studies suggested that ASCs, especially mesenchymal stem cells (MSCs) derived from bone marrow (BM), have intrinsic neurogenic potential and may be differentiated into mature cells of ectodermal origin [6-8].”
BackgroundChen, C., Xu, Y., & Song, Y. (2013) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-013-1855-8 · Cited by 39 · cited 1×
“Some research suggests that the MSCs express the nestin when they are under stress [21,22].”
Background“Stemness”: Definitions, Criteria, and Standards
Melton, D. A., & Cowen, C. (2009) · Elsevier eBooks · doi:10.1016/b978-0-12-374729-7.00083-4 · Cited by 37
Hong, S.-Q., Zhang, H., You, J., Zhang, M., Cai, Y., Jiang, X., & Xu, R. (2011) · Neurochemical Research · doi:10.1007/s11064-011-0567-2 · Cited by 32
Yun, S. J., Byun, K., Bhin, J., Oh, J., Nhung, L. T. H., Hwang, D., & Lee, B. (2010) · Journal of Cellular Physiology · doi:10.1002/jcp.22294 · Cited by 21 · cited 1×
“Background Stem cells are generally defined as immature cells with self-renewal competencies and the capability of differentiating into other cell types [1,2].”
BackgroundAndrogenetic Embryonic Stem Cells Form Neural Progenitor Cells In Vivo and In Vitro
Dinger, T. C., Eckardt, S., Choi, S. W., Camarero, G., Kurosaka, S., Hornich, V., McLaughlin, K. J., & Müller, A. M. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0877 · Cited by 20 · cited 1×
“ESCs are pluripotent cells derived from the inner cell mass of the blastocyst [4], whereas ASCs are isolated from adult tissues and defined as multipotent, since they are determined to produce cell types within their niche [5].”
BackgroundFirth, A. L., & Yuan, J. X. ‐. (2012) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00160.2012 · Cited by 10 · cited 2×
“IGF-1 has mitogenic effects by promoting G1/S cell cycle progression through Akt-dependent and tyrosine kinase– signalling pathways [23-25].”
Background
Cited by
Sorted by citations
BDNF as a Promising Therapeutic Agent in Parkinson’s Disease
Palasz, E., Wysocka, A., Gasiorowska, A., Chalimoniuk, M., Niewiadomski, W., & Niewiadomska, G. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031170 · Cited by 504
“Sci. 2020, 21, 1170 12 of 23 Akt Akt enzyme, also known as protein kinase B ALS amyotrophic lateral sclerosis ASN alpha-synuclein BBB blood-brain-barrier BDNF brain-derived neurotrophic factor CaMKII calmodulin-dependent protein kinase II cAMP cyclicAMP CNS central nervous system CREB cAMP-response element-binding pro…”
BackgroundDyer, A. H., Vahdatpour, C., Sanfeliu, A., & Tropea, D. (2016) · Neuroscience · doi:10.1016/j.neuroscience.2016.03.056 · Cited by 379
Biomarkers of postoperative delirium and cognitive dysfunction
Androsova, G., Krause, R., Winterer, G., & Schneider, R. (2015) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2015.00112 · Cited by 266
“Similarly to BDNF, insulin growth factor-1 (IGF-1) promotes neuronal proliferation, development, survival and enhanced synaptic transmission in CNS (Frost et al., 2003; Shcheglovitov et al., 2013; Huat et al., 2014).”
The Roles of Insulin-Like Growth Factors in Mesenchymal Stem Cell Niche
Youssef, A., Aboalola, D. A., & Han, V. K. M. (2017) · Stem Cells International · doi:10.1155/2017/9453108 · Cited by 144
Mesenchymal stem cell therapy for the treatment of traumatic brain injury: progress and prospects
Das, M., Mayilsamy, K., Mohapatra, S. S., & Mohapatra, S. (2019) · Reviews in the Neurosciences · doi:10.1515/revneuro-2019-0002 · Cited by 131
Metadata
- Authors
- Huat, T. J., Khan, A. A., Pati, S., Mustafa, Z., Abdullah, J. M., & Jaafar, H.
- Affiliations
- Universiti Sains Malaysia (MY)
- Corresponding author
- Huat, Tee Jong
- Venue
- BMC Neuroscience
- Published
- 2014-07-22
- Volume
- 15
- Issue
- 1
- Pages
- 91-91
- ISSN
- 1471-2202 (ISSN-L)
- PMC
- PMC4117972
- OpenAlex
- W2045159954
- Cited by
- 129
- References
- 29
- Citations by year
- 2026: 1 · 2025: 10 · 2024: 9 · 2023: 7 · 2022: 7 · 2021: 13 · 2020: 20 · 2019: 13 · 2018: 9 · 2017: 14 · 2016: 14 · 2015: 9 · 2014: 3
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Neuroscience (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Greater South Information System
- Funders
- Universiti Sains Malaysia
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- insulin-like growth factor I, mesenchymal stem cells, neural progenitor-like cells, neuronal differentiation, cell proliferation, apoptosis, growth factor combination, epidermal growth factor, basic fibroblast growth factor, LiF, brain-derived neurotrophic factor, nestin, neurospheres, neurodegenerative disease treatment
- MeSH terms
- Animals, Cell Survival, Cells, Cultured, Insulin-Like Growth Factor I, Neuroglia, Neurons, Rats, Sprague-Dawley, Apoptosis, Intercellular Signaling Peptides and Proteins, Cell Proliferation, Neurogenesis, Neural Stem Cells, Mesenchymal Stem Cells, Nestin
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