The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer
Open accessAbstract
Background: The identification of cancer-associated long non-coding RNAs and the investigation of their molecular and biological functions are important for understanding the molecular biology and progression of cancer. HOTAIR (HOX transcript antisense intergenic RNA) has been implicated in several cancers; however, its role in non-small cell lung cancer (NSCLC) is unknown. The aim of the present study was to examine the expression pattern of HOTAIR in NSCLC and to evaluate its biological role and clinical significance in tumor progression. Methods: Expression of HOTAIR was analyzed in 42 NSCLC tissues and four NSCLC cell lines by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Over-expression and RNA interference (RNAi) approaches were used to investigate the biological functions of HOTAIR. The effect of HOTAIR on proliferation was evaluated by MTT and colony formation assays, and cell migration and invasion were evaluated by transwell assays. Tail vein injection of cells was used to study metastasis in nude mice. Protein levels of HOTAIR targets were determined by western blot analysis. Differences between groups were tested for significance using Student’s t-test (two-tailed). Results: HOTAIR was highly expressed both in NSCLC samples and cell lines compared with corresponding normal counterparts. HOTAIR upregulation was correlated with NSCLC advanced pathological stage and lymph-node metastasis. Moreover, patients with high levels of HOTAIR expression had a relatively poor prognosis. Inhibition of HOTAIR by RNAi decreased the migration and invasion of NSCLC cells in vitro and impeded cell metastasis in vivo. HOXA5 levels were affected by HOTAIR knockdown or over-expression in vitro. Conclusions: Our findings indicate that HOTAIR is significantly up-regulated in NSCLC tissues, and regulates NSCLC cell invasion and metastasis, partially via the down-regulation of HOXA5. Thus, HOTAIR may represent a new marker of poor prognosis and is a potential therapeutic target for NSCLC intervention.
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Cites
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Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis
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Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
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Long Noncoding RNA as Modular Scaffold of Histone Modification Complexes
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“HOTAIR can also interact with a second histone modification complex, the LSD1/CoREST/REST complex, which coordinates the targeting of PRC2 and LSD1 to chromatin for coupled histone H3K27 methylation and K4 demethylation [22].”
BackgroundKhalil, A. M., Guttman, M., Huarte, M., Garber, M., Raj, A., Rivea Morales, D., Thomas, K., Presser, A., Bernstein, B. E., van Oudenaarden, A., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0904715106 · Cited by 2,872
Cited by
Functions of lncRNA HOTAIR in lung cancer
Loewen, G., Jayawickramarajah, J., Zhuo, Y., & Shan, B. (2014) · Journal of Hematology & Oncology · doi:10.1186/s13045-014-0090-4 · Cited by 454
Nie, F.-Q., Sun, M., Yang, J.-S., Xie, M., Xu, T.-P., Xia, R., Liu, Y.-W., Liu, X.-H., Zhang, E.-B., Lu, K.-H., et al. (2015) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-14-0492 · Cited by 375
Self-citationZhao, W., Geng, D., Li, S., Chen, Z., & Sun, M. (2018) · Cancer Medicine · doi:10.1002/cam4.1353 · Cited by 375
Self-citationRetractedLong Noncoding RNAs as Biomarkers in Cancer
Bolha, L., Ravnik-Glavač, M., & Glavač, D. (2017) · Disease Markers · doi:10.1155/2017/7243968 · Cited by 365
Long noncoding RNAs: a potential novel class of cancer biomarkers
Yarmishyn, A. A., & Kurochkin, I. V. (2015) · Frontiers in Genetics · doi:10.3389/fgene.2015.00145 · Cited by 273
Metadata
- Authors
- Liu, X.-H., Liu, Z.-L., Sun, M., Liu, J., Wang, Z.-X., & De, W.
- Venue
- BMC Cancer
- Published
- 2013-10-08
- Volume
- 13
- Issue
- 1
- Pages
- 464-464
- ISSN
- 1471-2407 (ISSN-L)
- OpenAlex
- W2148050145
- Cited by
- 391
- References
- 34
- FWCI
- 12.52
- Type
- journal article
- Language
- en
- Open access
- gold
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