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Cited by 161Phytochemicals and Medicinal PlantsEssential Oils and Antimicrobial ActivityMedicinal Plant Research

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities

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Alam, Nadia, Hossain, Monzur, Mottalib, Md Abdul, Sulaiman, Siti Amrah, Gan, Siew Hua, Khalil, Md Ibrahim·2012·BMC Complementary and Alternative Medicine
DOI 10.1186/1472-6882-12-175PMC PMC3527235
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Abstract

BACKGROUND: Withania somnifera, also known as ashwagandha, is an important herb in ayurvedic and indigenous medical systems. The present study was designed to evaluate the antioxidant and antibacterial activities of an 80% aqueous methanolic extract of W. somnifera roots (WSREt), fruits (WSFEt) and leaves (WSLEt). METHODS: Several assays were performed to determine the antioxidant properties of this herb including 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity, ferric reducing antioxidant power (FRAP), ferrous chelation and inhibition of β-carotene bleaching. RESULTS: The values for DPPH, FRAP, ferrous chelation and inhibition of β carotene bleaching for the three types of extracts ranged from 101.73-801.93 μg/ml, 2.26-3.29 mM Fe/kg, 0.22-0.65 mg/ml and 69.87-79.67%, respectively, indicating that W. somnifera, particularly the leaves, possesses significant antioxidant properties. The mean ascorbic acid content was 20.60-62.60 mg/100 g, and the mean anthocyanin content was 2.86-12.50 mg/100 g. Antibacterial activities were measured using the agar well diffusion method and five pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhi, Citrobacter freundii, Pseudomonas aeruginosa and Klebsiella pneumoniae. The leaf extracts displayed the highest activity against S. typhi (32.00 ± 0.75 mm zone of inhibition), whereas the lowest activity was against K. pneumoniae (19.00 ± 1.48 mm zone of inhibition). The lowest minimum inhibitory concentration value was 6.25 mg/ml, which was against S. typhi, followed by 12.5 mg/ml against E. coli. CONCLUSION: In addition to its antioxidant properties, W. somnifera exhibited significant antibacterial activities against Gram-negative bacteria, particularly S. typhi.

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Research works printed but not linked: 7

  1. Ostrowska B, Rzemy KZ: Antioxidant effects a basis of drug selection. Herba Pol. 1998, 44 (4): 417-
  2. Jain P, Varshney R: Antimicrobial activity of aqueous and methanolic extracts of Withania somnifera (Ashwagandha). J Chem Pharmaceut Res. 2011, 3 (3): 260-263.
  3. Ronald M, Atlas: Micro-organisms in our World. 1995, St. Louis, Missouri: Mosby-Year Book, Inc, 765 blz-
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  5. Ibekwe VI, Nnanyere NF, Akujobi CO: Studies on Antibacterial activity and phytochemical qualities of various plant extracts. Int J Environ, Health and Human Development. 2001, 2 (1): 41-46.
  6. Hussain I, Khan L, Marwat GA: Analysis of Vitamin C in Selected Medicinal Plants. J Chem Soc Pakistan. 2011, 33 (2): 260-262.
  7. Ramarathnam N, Ochi H, Takeuchi M: Antioxidant defense system in vegetable extracts. Natural Antioxidants; Chemistry, Health Effects and Application 1997. Edited by: Shahidi F. 1997, Champaign, IL: AOAC Press, 76-87.

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Metadata

Authors
Alam, N., Hossain, M., Mottalib, M. A., Sulaiman, S. A., Gan, S. H., & Khalil, M. I.
Affiliations
  • University of Rajshahi (BD)
  • Bangladesh Institute of Research and Rehabilitation for Diabetes Endocrine and Metabolic Disorders (BD)
  • Universiti Sains Malaysia (MY)
Corresponding author
Khalil, Md Ibrahim
ORCID
Venue
BMC Complementary and Alternative Medicine
Publisher
Springer Science and Business Media LLC
Published
2012-10-07
Volume
12
Issue
1
Pages
175-175
ISSN
1472-6882 (ISSN-L)
DOI
10.1186/1472-6882-12-175
PMC
PMC3527235
OpenAlex
W1971728963
Cited by
159
References
38
Citations by year
2026: 11 · 2025: 15 · 2024: 13 · 2023: 14 · 2022: 18 · 2021: 19 · 2020: 12 · 2019: 13 · 2018: 3 · 2017: 22 · 2016: 6 · 2015: 10 · 2014: 2
FWCI
9.34
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Complementary and Alternative Medicine (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Greater South Information System
Funders
Universiti Sains Malaysia (1001/PPSP/815058)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
Withania somnifera, methanol extract, antioxidant activity, antibacterial activity, Gram-negative bacteria, Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Citrobacter freundii, DPPH radical scavenging, vitamin C, anthocyanins, minimum inhibitory concentration, Ayurvedic herbs
MeSH terms
Anthocyanins, Anti-Bacterial Agents, Antioxidants, Ascorbic Acid, Gram-Negative Bacteria, Microbial Sensitivity Tests, Plant Extracts, Plant Leaves, Withania

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