Antioxidant activity of plant extracts containing phenolic compounds
Abstract
The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials (berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds) was examined by autoxidation of methyl linoleate. The content of total phenolics in the extracts was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). Among edible plant materials, remarkable high antioxidant activity and high total phenolic content (GAE > 20 mg/g) were found in berries, especially aronia and crowberry. Apple extracts (two varieties) showed also strong antioxidant activity even though the total phenolic contents were low (GAE < 12.1 mg/g). Among nonedible plant materials, high activities were found in tree materials, especially in willow bark, spruce needles, pine bark and cork, and birch phloem, and in some medicinal plants including heather, bog-rosemary, willow herb, and meadowsweet. In addition, potato peel and beetroot peel extracts showed strong antioxidant effects. To utilize these significant sources of natural antioxidants, further characterization of the phenolic composition is needed.
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Phenolic compounds are commonly classified into 5 large groups: flavonoids, lignans, tannins, tocopherols, and phenolic acids.
“Phenolic compounds are commonly classified into 5 large groups: Flavonoids (the largest subgroup and associated with different bioactivities, among which, as previously described, the antioxidant, and radical scavenging activity) [158,159], lignans, tannins, tocopherols, and phenolic acids [160].”
From The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value (2019) · cites this paper as [158]
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“In addition to protective functions, phenolic compounds—in particular anthocyanins—are responsible for the pigmentation of flowers, fruit and leaves [33, 44].”
BackgroundAssessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants
Maisuthisakul, P., Suttajit, M., & Pongsawatmanit, R. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2005.11.032 · Cited by 641 · see citing works
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“The interest in phenolic compounds derived from vegetables and their roles in nutrition are therefore increasing [32, 37].”
BackgroundEffect of Temperatures on Polyphenols during Extraction
Antony, A., & Farid, M. (2022) · Applied Sciences · doi:10.3390/app12042107 · Cited by 654 · see citing works
Hoffmann, L., Besseau, S., Geoffroy, P., Ritzenthaler, C., Meyer, D., Lapierre, C., Pollet, B., & Legrand, M. (2004) · The Plant Cell · doi:10.1105/tpc.020297 · Cited by 582 · see citing works
“Among the myriad of plant natural products, compounds issuing from the phenylpropanoid pathway (Figure 1) have been reported to have antioxidant effects, estrogen-like and vasodilatation activities, and anti-inflammatory and anticancer chemopreventive action (Jang et al., 1997; Kahkonen et al., 1999; Burns et al., 200…”
BackgroundKell, D. B. (2009) · BMC Medical Genomics · doi:10.1186/1755-8794-2-2 · Cited by 554 · see citing works
“This is reasonable given that many of these polyphenols and flavonoid compounds [1650; 1673-1682] have groups such as the catechol moiety that are part of the known iron-binding elements of microbial siderophores.”
BackgroundPolyphenols: food sources, properties and applications – a review
Gharras, H. E. (2009) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2009.02077.x · Cited by 566 · see citing works
Natural Bioactive Compounds from Winery By-Products as Health Promoters: A Review
Teixeira, A., Baenas, N., Domínguez‐Perles, R., Barros, A., Rosa, E. A. S., Moreno, D. A., & García‐Viguera, C. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms150915638 · Cited by 544 · see citing works
“including phenolic acids (hydroxybenzoic acids and hydroxycinnamic acids), flavonoids (flavanols or flavan-3-ols, proanthocyanidins, flavones, and flavonols), and stilbenes [72], which have been reported as responsible for multiple biological effects [73].”
BackgroundHäkkinen, S. H., & Törrönen, A. (2000) · Food Research International · doi:10.1016/s0963-9969(00)00086-7 · Cited by 538 · see citing works
Nuutila, A. M., Puupponen‐Pimiä, R., Aarni, M., & Oksman‐Caldentey, K. (2003) · Food Chemistry · doi:10.1016/s0308-8146(02)00476-4 · Cited by 533 · see citing works
Sultana, B., Anwar, F., & Przybylski, R. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.01.019 · Cited by 533 · see citing works
“Phenolic compounds are not evenly distributed in plant parts; they are present at elevated amounts in the outer parts of the fruits, leaves and barks (Kahkonen et al., 1999).”
BackgroundAntioxidants in tomato (Lycopersium esculentum) as a function of genotype
George, B., Kaur, C., Khurdiya, D. S., & Kapoor, H. C. (2003) · Food Chemistry · doi:10.1016/s0308-8146(03)00165-1 · Cited by 529 · see citing works
Kujala, T. S., Loponen, J. M., Klika, K. D., & Pihlaja, K. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000523q · Cited by 525 · see citing works
Antioxidant Activity of Dietary Fruits, Vegetables, and Commercial Frozen Fruit Pulps
Hassimotto, N. M. A., Genovese, M. I., & Lajolo, F. M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf047894h · Cited by 523 · see citing works
Kulling, S. E., & Rawel, H. M. (2008) · Planta Medica · doi:10.1055/s-0028-1088306 · Cited by 517 · see citing works
“These assays include the inhibition of methyl linoleate oxidation [39], [43], oxygen radical absorbance capacity (ORAC) [47], [63], trolox equivalence antioxidant capacity (TEAC) [45], [64], [65], [66], ferric ion reducing antioxidant power (FRAP) [67] as well as the antiradical capacity using DPPH…”
BackgroundMethodLiyana‐Pathirana, C. M., & Shahidi, F. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf049320i · Cited by 506 · see citing works
An, K., Zhao, D., Wang, Z., Wu, J., Xu, Y., & Xiao, G. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.11.033 · Cited by 500 · see citing works
Several Culinary and Medicinal Herbs Are Important Sources of Dietary Antioxidants
Dragland, S., Senoo, H., Wake, K., Holte, K., & Blomhoff, R. (2003) · Journal of Nutrition · doi:10.1093/jn/133.5.1286 · Cited by 498 · see citing works
Proestos, C., Boziaris, I. S., Nychas, G. E., & Komaitis, M. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.01.049 · Cited by 490 · see citing works
“They also have metal chelation properties (Kähkönen et al., 1999; Rice-Evans, Miller, & Paganga, 1997).”
BackgroundMethodInfluentialAntioxidant Activity of Extracts from Acacia confusa Bark and Heartwood
Chang, S., Wu, J., Wang, S., Kang, P.-L., Yang, N., & Shyur, L. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0100907 · Cited by 490 · see citing works
Antioxidant Activity of Spices and Their Impact on Human Health: A Review
Yashin, A. Y., Yashin, Y. I., Xia, X., & Nemzer, B. V. (2017) · Antioxidants · doi:10.3390/antiox6030070 · Cited by 509 · see citing works
“[59] measured the antioxidant activity of different plant extracts spectrometrically according to the Folin–Ciocalteu procedure and found the total phenolic content of bog-rosemary as calculated as GAE was even higher than that of all the vegetables and some fruits studied at the same time.”
MethodPotential Impact of Strawberries on Human Health: A Review of the Science
Hannum, S. M. (2004) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408690490263756 · Cited by 488 · see citing works
“44 Their lab measured phenolic content by means of the Folin-Ciocalteu procedure and measured antioxidant activity by the methyl linoleate (MeLo) method.”
MethodParejo, I., Viladomat, F., Bastida, J., Rosas-Romero, A., Flerlage, N., Burillo, J., & Codina, C. J. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020540a · Cited by 473 · see citing works
Bioactive compounds and antioxidant potential of mango peel extract
Ajila, C. M., Naidu, K. A., BHAT, S., & Rao, U. J. S. P. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.04.052 · Cited by 467 · see citing works
“The increased interest in natural antioxidants has led to the antioxidant evaluation of many species of fruits, vegetables, herbs, spices and cereals (Kahkonen et al., 1999; Liyana-Pathirana & Shahidi, 2005; Velioglu, Mazza, Gao, & Oomah, 1998; Wolfe, Xianzhong, & Liu, 2003).”
BackgroundIndian medicinal herbs as sources of antioxidants
Ali, S. S., Kasoju, N., Luthra, A., Singh, A., Hallihosur, S., Sahu, A., & Bora, U. (2007) · Food Research International · doi:10.1016/j.foodres.2007.10.001 · Cited by 460 · see citing works
“Antioxidants derived from fruits, vegetables, spices and cereals are very effective and have reduced interference with the body’s ability to use free radicals constructively (Kahkonen et al., 1999; Wolfe, Xianzhong, & Liu, 2003).”
BackgroundDetermination of antioxidant activity and antioxidant compounds in wild edible mushrooms
Elmastaş, M., Isildak, O., Türkekul, İ., & Temur, N. (2006) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2006.07.003 · Cited by 457 · see citing works
Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods
Lim, Y. Y., & Murtijaya, J. (2007) · LWT · doi:10.1016/j.lwt.2006.12.013 · Cited by 456 · see citing works
“The total phenolic content (TPC) of the plant extracts was determined spectrophotometrically using Folin-Ciocalteau’s reagent according to the method described by Kahkonen et al. (1999) with slight modifications.”
MethodAntioxidant activity and constituents of propolis collected in various areas of China
Ahn, M., Kumazawa, S., Usui, Y., Nakamura, J., Matsuka, M., Zhu, F., & Nakayama, T. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.03.045 · Cited by 457 · see citing works
Antioxidant activity of three edible seaweeds from two areas in South East Asia
Chew, Y.-L., Lim, Y. Y., Omar, M., & Khoo, K. S. (2007) · LWT · doi:10.1016/j.lwt.2007.06.013 · Cited by 455 · see citing works
Mathew, S., & Abraham, T. E. (2005) · Food and Chemical Toxicology · doi:10.1016/j.fct.2005.06.013 · Cited by 452 · see citing works
“lipids and thereby improve the quality and nutritive value of food (Kähkönen et al., 1999; Rice Evans et al., 1995).”
BackgroundSeparation procedures for naturally occurring antioxidant phytochemicals
Tsao, R., & Deng, Z. (2004) · Journal of Chromatography B · doi:10.1016/j.jchromb.2004.09.028 · Cited by 448 · see citing works
Proestos, C., Chorianopoulos, N., Nychas, G. E., & Komaitis, M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf040083t · Cited by 447 · see citing works
Aronia melanocarpa phenolics and their antioxidant activity
Oszmiański, J., & Wojdyło, A. (2005) · European Food Research and Technology · doi:10.1007/s00217-005-0002-5 · Cited by 447 · see citing works
Anthocyanin and antioxidant capacity in Roselle (Hibiscus Sabdariffa L.) extract
Tsai, P., McIntosh, J., Pearce, P. D., et al. (2002) · Food Research International · doi:10.1016/s0963-9969(01)00129-6 · Cited by 446 · see citing works
Hayouni, E. A., Abderrabba, M., Bouix, M., & Hamdi, M. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.02.010 · Cited by 442 · see citing works
Nutritional composition of five cultivars of chinese jujube
Li, J., Fan, L., Ding, S., & Ding, X. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.08.016 · Cited by 420 · see citing works
“However, Ismail, Marjan, and Foong (2004) reported no correlation between total phenolic contents and antioxidant capacities of extracts from five types of vegetables, nor were any relationships between antioxidant capacities and phenolic composition found in fruit berry, fruit wines (Heinonen, Meyer, & Frankel, 1998)…”
BackgroundMeneses, N. G. T., Martins, S., Teixeira, J. A., & Mussatto, S. I. (2013) · Separation and Purification Technology · doi:10.1016/j.seppur.2013.02.015 · Cited by 423 · see citing works
“[44] who reported that the antioxidant activity of a phenolic extract cannot be predicted only on the basis of its total phenolic content, but it can be influenced by specific phenolic compounds present in this mixture.”
BackgroundPhenolic contents and antioxidant activity of some food and medicinal plants
Bajpai, M., Pande, A., Tewari, S. K., & Prakash, D. (2005) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637480500146606 · Cited by 418 · see citing works
“Certain plants are natural sources of phenols and may be used as antioxidants (Kähkönen et al. 1999).”
BackgroundInfluentialAntioxidant activity of fresh and dry herbs of some Lamiaceae species
Capecka, E., Mareczek, A., & Leja, M. (2004) · Food Chemistry · doi:10.1016/j.foodchem.2004.09.020 · Cited by 417 · see citing works
Polyphenolic Compounds and Antioxidant Activity of New and Old Apple Varieties
Wojdyło, A., Oszmiański, J., & Laskowski, P. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf800510j · Cited by 402 · see citing works
Berries extracts as natural antioxidants in meat products: A review
Lorenzo, J. M., Pateiro, M., Domínguez, R., Barba, F. J., Putnik, P., Kovačević, D. B., Shpigelman, A., Granato, D., & Franco, D. (2017) · Food Research International · doi:10.1016/j.foodres.2017.12.005 · Cited by 400 · see citing works
Bahorun, T., Luximon‐Ramma, A., Crozier, A., & Aruoma, O. I. (2004) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1820 · Cited by 399 · see citing works
Importance of Insoluble-Bound Phenolics to Antioxidant Properties of Wheat
Liyana‐Pathirana, C. M., & Shahidi, F. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf052556h · Cited by 399 · see citing works
Anthocyanins—More Than Nature′s Colours
Konczak, I., & Zhang, W. Y. (2004) · BioMed Research International · doi:10.1155/s1110724304407013 · Cited by 388 · see citing works
Distribution of Conjugated and Free Phenols in Fruits: Antioxidant Activity and Cultivar Variations
Imeh, U., & Khokhar, S. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020342j · Cited by 383 · see citing works
Relative antioxidant and cytoprotective activities of common herbs
Yoo, K. M., Lee, C. H., Lee, H., et al. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.07.006 · Cited by 379 · see citing works
“Bioactive compounds commonly found in fruits, vegetables, herbs, and other plants have been shown to have possible health benefits with antioxidative, anticarcinogenic, atherosclerosis, antimutagenic, and angiogenesis inhibitory activities (Cao & Cao, 1999; Geleijnse, Launer, Hofman, Pols, & Witteman, 1999; Kahkonen e…”
InfluentialA review: using nanoparticles to enhance absorption and bioavailability of phenolic phytochemicals
Li, Z., Jiang, H., Xu, C., & Gu, L. (2015) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2014.05.010 · Cited by 376 · see citing works
Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds
Proestos, C., & Komaitis, M. (2007) · LWT · doi:10.1016/j.lwt.2007.04.013 · Cited by 375 · see citing works
“The spectrophotometric measurement was repeated three times for each extract and the average data was interpolated in a gallic acid calibration curve and expressed as mg of gallic acid per g of dry sample (Kähkönen et al., 1999).”
MethodOn the extraction and antioxidant activity of phenolic compounds from winery wastes
Lafka, T.-I., Sinanoglou, V. J., & Lazos, E. S. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.01.068 · Cited by 374 · see citing works
Effects of ultrasound treatments on quality of grapefruit juice
Aadil, R. M., Zeng, X., Han, Z., et al. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.06.008 · Cited by 371 · see citing works
“Phenolic compounds are one of the main contributors in antioxidant activity in fruit juices and polyphenolic compounds are commonly found in both edible and inedible plants which have been reported to have multiple biological effects and antioxidant activities (Van Acker et al., 1996; Kähkönen et al., 1999).”
BackgroundAntioxidant activity and antimicrobial effect of berry phenolics – a Finnish perspective
Heinonen, M. (2007) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200700006 · Cited by 367 · see citing works
Peterson, D. M. (2001) · Journal of Cereal Science · doi:10.1006/jcrs.2000.0349 · Cited by 366 · see citing works
Rodríguez-Carpena, J. G., Morcuende, D., Andrade, M. J., Kylli, P., & Estévez, M. (2011) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf1048832 · Cited by 362 · see citing works
Antioxidant nutritional quality of tomato
Frusciante, L., Carli, P., Ercolano, M. R., Pernice, R., Matteo, A. D., Fogliano, V., & Pellegrini, N. (2007) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200600158 · Cited by 362 · see citing works
“[14] reported that the total phenolic content of tomatoes is up to 200 mg of gallic acid equivalent per 100 g (as dried weight).”
BackgroundQuantitative analysis of antiradical phenolic constituents from fourteen edible Myrtaceae fruits
Reynertson, K. A., Yang, H., Jiang, B., Basile, M. J., & Kennelly, E. J. (2008) · Food Chemistry · doi:10.1016/j.foodchem.2008.01.021 · Cited by 355 · see citing works
“Analysis was performed by the Folin-Ciocalteu method (Singleton & Rossi, 1965; Kähkönen et al., 1999).”
MethodAn Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds
Cox, S., Abu-Ghannam, N., & Gupta, S. (2010) · PubMed · doi:10.21427/d7hc92 · Cited by 353 · see citing works
“Kahkonen et al. (1999) stated that flavonoids are probably the most important natural phenolics due to their broad spectrum of chemical and biological activities, including antioxidant and free radical scavenging properties.”
BackgroundAntioxidant capacities and phenolic compounds of the husk, bran and endosperm of Thai rice
Butsat, S., & Siriamornpun, S. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2009.07.001 · Cited by 353 · see citing works
Quality characteristics of sesame seeds and by-products
Elleuch, M., Besbes, S., Roiseux, O., Blecker, C. S., & Attia, H. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.09.008 · Cited by 358 · see citing works
“Indeed, it was established that coats of vegetable seeds, coats of cereal grains and peels of fruits (characterized by the high dietary fibre content) contain higher amounts of polyphenols than the cotyledon, the endosperm and the pulp fractions respectively (Dueñas, Hernández, & Estrella, 2002; Gorinstein et al., 200…”
BackgroundVázquez, G., Fontenla, E., Santos, J., Freire, M. S., González‐Álvarez, J., & Antorrena, G. (2008) · Industrial Crops and Products · doi:10.1016/j.indcrop.2008.03.003 · Cited by 352 · see citing works
In vitro antioxidant activities of Stevia rebaudiana leaves and callus
., M. T., Patel, V. H., & Subhash, R. (2007) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2006.08.004 · Cited by 351 · see citing works
“Phenolic compounds are commonly found in both edible and non-edible plants, and they have been reported to have multiple biological effects, including antioxidant activity (Kahkonen et al., 1999).”
BackgroundInfluentialProduction of Antioxidant High Dietary Fiber Powder from Carrot Peels
Chantaro, P., Devahastin, S., & Chiewchan, N. (2008) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2007.11.013 · Cited by 346 · see citing works
Aromatic plants as a source of bioactive compounds
Christaki, E., Bonos, E., Giannenas, I., & Florou-Paneri, P. (2012) · Agriculture · doi:10.3390/agriculture2030228 · Cited by 346 · see citing works
“Aromatic plants and their essential oils are good sources of natural antioxidants, such as phenolic compounds, e.g., eugenol, thymol, carvacrol [58,59].”
Antioxidant and tyrosinase inhibition properties of leaves and rhizomes of ginger species
Chan, E. W. C., Lim, Y. Y., Wong, L. F., et al. (2008) · Food Chemistry · doi:10.1016/j.foodchem.2008.02.016 · Cited by 344 · see citing works
Antioxidant evaluation protocols: Food quality or health effects
Becker, E. M., Nissen, L. R., & Skibsted, L. H. (2004) · European Food Research and Technology · doi:10.1007/s00217-004-1012-4 · Cited by 344 · see citing works
- Authors
- Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J.-P., Pihlaja, K., Kujala, T. S., & Heinonen, M.
- Affiliations
- University of Helsinki (FI)
- Corresponding author
- Kähkönen, Marja P.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-09-24
- Volume
- 47
- Issue
- 10
- Pages
- 3954-3962
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2138132897
- Cited by
- 3,854
- References
- 60
- Citations by year
- 2026: 95 · 2025: 119 · 2024: 113 · 2023: 143 · 2022: 154 · 2021: 200 · 2020: 192 · 2019: 168 · 2018: 205 · 2017: 174 · 2016: 206 · 2015: 228 · 2014: 239 · 2013: 209 · 2012: 226
- FWCI
- 14.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- antioxidant activity, phenolic compounds, plant extracts, total phenolic content, gallic acid equivalent, berries, aronia, crowberry, apple, medicinal plants, bark, potato peel, natural antioxidants
- MeSH terms
- Antioxidants, Linoleic Acids, Oxidation-Reduction, Phenols, Plant Extracts
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