Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae)
Open accessAbstract
Background: Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus. Methods: Fungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City, southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays. Results: A total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems. 53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF. 80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli. Conclusion: The secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.
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Tetrazolium salts, MTT and XTT are often used in MIC endpoint determination for both antifungal and antibacterial microdilution assays.
“Tetrazolium salts, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis {2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide} (XTT), are often used in the MIC endpoint determination for both antifungal and antibacterial microdilution assays [57], [58], [59], [60].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [58]
The Methods section ('In vitro antimicrobial activity'; page not provided) describes using MTT in a microtiter-plate assay of bacteria and a fungus and assigning an MIC endpoint. It does not mention XTT or establish that both salts are often used for these assays. (LLM, conf=0.87)
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- Authors
- Liang, H., Xing, Y., Chen, J., Zhang, D., Guo, S., & Wang, C.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Wang, Chunlan
- Venue
- BMC Complementary and Alternative Medicine
- Published
- 2012-11-28
- Volume
- 12
- Issue
- 1
- Pages
- 238-238
- ISSN
- 1472-6882 (ISSN-L)
- PMC
- PMC3534486
- OpenAlex
- W2164806808
- Cited by
- 108
- References
- 45
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 8 · 2023: 10 · 2022: 12 · 2021: 11 · 2020: 12 · 2019: 15 · 2018: 6 · 2017: 7 · 2016: 5 · 2015: 3 · 2014: 9 · 2013: 2
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Complementary and Alternative Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31170016, 31070300)
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
- Health Sciences › Medicine › Pharmacology › Biological and pharmacological studies of plants
- Keywords
- endophytic fungi, Ophiopogon japonicus, antimicrobial activity, Staphylococcus aureus, Cryptococcus neoformans, secondary metabolites, mycelial extract, fermentation broth, traditional Chinese medicinal plants, antibacterial agents
- MeSH terms
- Anti-Infective Agents, Bacteria, Cryptococcus neoformans, Fungi, Molecular Sequence Data, Phylogeny, Ophiopogon, Endophytes
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