Cited by 108Microbial Natural Products and BiosynthesisPlant and fungal interactionsBiological and pharmacological studies of plants

Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae)

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Liang, Hanqiao, Xing, Yongmei, Chen, Juan, Zhang, Dawei, Guo, Shunxing, Wang, Chunlan·2012·BMC Complementary and Alternative Medicine

Abstract

Background: Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus. Methods: Fungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City, southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays. Results: A total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems. 53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF. 80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli. Conclusion: The secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.

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Crossref · 10.1186/1472-6882-12-238

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  • Tetrazolium salts, MTT and XTT are often used in MIC endpoint determination for both antifungal and antibacterial microdilution assays.

    “Tetrazolium salts, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis {2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide} (XTT), are often used in the MIC endpoint determination for both antifungal and antibacterial microdilution assays [57], [58], [59], [60].”

    From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [58]

    The Methods section ('In vitro antimicrobial activity'; page not provided) describes using MTT in a microtiter-plate assay of bacteria and a fungus and assigning an MIC endpoint. It does not mention XTT or establish that both salts are often used for these assays. (LLM, conf=0.87)

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Research works printed but not linked: 3

  1. Flora of China Editorial Committee of Chinese Academy of Sciences Flora of China 1978 Beijing: Science Press 130 164
  2. Lin D Study on allelopathy of dwarf lilyturf (Ophiopogon japonicus Ker-Gawl.) MS thesis 2001 Japan: Miyazaki University 15 47
  3. Nakanishi Y Kaneda H The chemical constitution research of Ophiopogon (Chinese) Yakugaku Zasshi 1987 107 780 784 3440921

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Authors
Liang, H., Xing, Y., Chen, J., Zhang, D., Guo, S., & Wang, C.
Affiliations
Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
Corresponding author
Wang, Chunlan
Venue
BMC Complementary and Alternative Medicine
Published
2012-11-28
Volume
12
Issue
1
Pages
238-238
ISSN
1472-6882 (ISSN-L)
OpenAlex
W2164806808
Cited by
108
References
45
Citations by year
2026: 3 · 2025: 5 · 2024: 8 · 2023: 10 · 2022: 12 · 2021: 11 · 2020: 12 · 2019: 15 · 2018: 6 · 2017: 7 · 2016: 5 · 2015: 3 · 2014: 9 · 2013: 2
FWCI
3.29
Type
journal article
Language
en
Open access
Locations
  • BMC Complementary and Alternative Medicine (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China (31170016, 31070300)
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
  • Health Sciences › Medicine › Pharmacology › Biological and pharmacological studies of plants
Keywords
endophytic fungi, Ophiopogon japonicus, antimicrobial activity, Staphylococcus aureus, Cryptococcus neoformans, secondary metabolites, mycelial extract, fermentation broth, traditional Chinese medicinal plants, antibacterial agents
MeSH terms
Anti-Infective Agents, Bacteria, Cryptococcus neoformans, Fungi, Molecular Sequence Data, Phylogeny, Ophiopogon, Endophytes

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