The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins
Open accessAbstract
Background: Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein. Results: By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis. Conclusion: This observation offers promising possibilities for the exploration of inclusion bodies as catalysts for industrial purposes, without any previous protein-refolding step.
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Citations
Cites
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
Sørensen, H. P., & Mortensen, K. K. (2005) · Microbial Cell Factories · doi:10.1186/1475-2859-4-1 · Cited by 841 · cited 1×
“In this context, soluble micro-aggregates have been described [11] and recently characterized in detail [12].”
Cited by
Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
Singh, A., Upadhyay, V., Upadhyay, A. K., Singh, S. M., & Panda, A. K. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0222-8 · Cited by 444
Protein quality in bacterial inclusion bodies
Ventura, S., & Villaverde, A. (2006) · Trends in Biotechnology · doi:10.1016/j.tibtech.2006.02.007 · Cited by 381
Self-citationImproved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli
Picaud, S., Olsson, M. E., & Brodelius, P. E. (2007) · Protein Expression and Purification · doi:10.1016/j.pep.2006.06.025 · Cited by 33
Wyre, C., & Overton, T. W. (2014) · Journal of Industrial Microbiology and Biotechnology · doi:10.1007/s10295-014-1489-1 · Cited by 27
Metadata
- Authors
- García-Fruitós, E., González-Montalbán, N., Morell, M., Vera, A., Ferraz, R. M., Arís, A., Ventura, S., & Villaverde, A.
- Venue
- Microbial Cell Factories
- Published
- 2005-09-12
- Volume
- 4
- Issue
- 1
- Pages
- 27-27
- ISSN
- 1475-2859 (ISSN-L)
- OpenAlex
- W2115668757
- Cited by
- 319
- References
- 1
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- gold
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- García-Fruitós, E., González-Montalbán, N., Morell, M., Vera, A., Ferraz, R. M., Arís, A., Ventura, S., & Villaverde, A. (2005). Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins. Microbial Cell Factories. https://doi.org/10.1186/1475-2859-4-27
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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