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Cited by 27Neuropeptides and Animal PhysiologyMaternal and Perinatal Health InterventionsPregnancy-related medical research

Reduced expression of CRH receptor type 1 in upper segment human myometrium during labour

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Cong, Binhai, Zhang, Lanmei, Gao, Lu, Ni, Xin·2009·Reproductive Biology and Endocrinology
DOI 10.1186/1477-7827-7-43PMC PMC2694199
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Abstract

Background: Corticotropin-releasing hormone (CRH) and CRH-related peptide are shown to modulate uterine contractility through two CRH receptor subtype, CRH-R1 and CRH-R2 during pregnancy. Through different signaling pathways, CRH-R1 maintains myometrial quiescence whereas CRH-R2 promotes smooth muscle contractility. We hypothesized that the expression of CRH receptors in myometrium might be changed during pregnancy and labour. Method: Immunohistochemistry, Western blot and RT-PCR were used to quantify the cellular localization, the protein levels and the mRNA variants of both CRH-R1 and CRH-R2 in upper segment (US) and lower segment (LS) myometrium from nonpregnant and pregnant women at term before or after labour. Results: CRH-R1 and CRH-R2 were predominately localized to myometrial smooth muscle cells in US and LS. The protein level of CRH-R1 in US was significantly down-regulated in pregnancy, with a further decrease at the onset of labour. However, the expression of CRH-R1 in LS remained unchanged during pregnancy and labour. No significant changes in CRH-R2 expression were observed in US or LS. Six variants of CRH-R1, CRH-R1alpha,-R1beta,-R1c, -R1e,-R1f and -R1g, were identified in nonpregnant and pregnant myometrium. CRH-R2alpha was identified in pregnant myometrium, whereas CRH-R2beta was identified in nonpregnant myometrium Conclusion: CRH-R1 and CRH-R2 are expressed in nonpregnant and pregnant US and LS myometrium. Changed expression of CRH receptors during labour may underlie the initiation of uterine contractility during parturition.

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  • The direct actions of CRH on myometrial contractility involve distinct CRH receptor isoforms.

    “The direct actions of CRH on myometrial contractility involve distinct CRH receptor isoforms.”

    From Stress and the HPA Axis: Balancing Homeostasis and Fertility. (2017) · cites this paper as [64]

  • Expression of CRH-R1 decreases in the upper segment of the uterus during labor, which becomes highly contractile.

    “However, expression of CRH-R1 decreases in the upper segment of the uterus during labor, which becomes highly contractile [64].”

    From Stress and the HPA Axis: Balancing Homeostasis and Fertility. (2017) · cites this paper as [64]

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Cited by

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Authors
Cong, B., Zhang, L., Gao, L., & Ni, X.
Affiliations
  • Second Military Medical University (CN)
  • PLA Navy General Hospital (CN)
Corresponding author
Ni, Xin
ORCID
Venue
Reproductive Biology and Endocrinology
Publisher
Springer Science and Business Media LLC
Published
2009-05-12
Volume
7
Issue
1
Pages
43-43
ISSN
1477-7827 (ISSN-L)
DOI
10.1186/1477-7827-7-43
PMC
PMC2694199
OpenAlex
W2048022501
Cited by
27
References
2
Citations by year
2025: 1 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 3
FWCI
1.39
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Reproductive Biology and Endocrinology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China
  • People’s Liberation Army Navy General Hospital
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Obstetrics and Gynecology › Maternal and Perinatal Health Interventions
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Pregnancy-related medical research
Keywords
corticotropin-releasing factor, CRH receptor type 1, myometrium, labour, uterine contractility, myometrial quiescence, pregnancy, term pregnancy, smooth muscle cells
MeSH terms
CRF Receptor, Type 1, Female, Humans, Immunohistochemistry, Labor, Obstetric, Myometrium, Pregnancy, RNA, Messenger, Uterine Contraction, Blotting, Western, Signal Transduction, Gene Expression, Alternative Splicing, Receptors, Corticotropin-Releasing Hormone

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