Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library
Open accessAbstract
BACKGROUND: Esophageal cancer is a common malignant tumor of the gastrointestinal tract and is typically diagnosed at an advanced stage due to the absence of early clinical symptoms. Although surgery, chemotherapy, and radiotherapy represent the major treatment methods employed for this cancer, the prognosis of esophageal cancer remains poor. METHODS: A Ph.D.-12TM Phage Display Peptide Library was screened using an esophageal cancer cell line, Eca109, and a normal esophageal epithelial cell line to identify novel ligands that selectively bind the surface of esophageal cancer cells with high affinity. RESULTS: Two polypeptides were isolated that exhibited higher binding affinities and specificity for the Eca109 cells. These peptides were further validated using enzyme-linked immunosorbent assays (ELISAs), immunofluorescence assays, and immunohistochemistry assays. CONCLUSION: Two polypeptides with high binding affinities to esophageal cancer cells were isolated from the Ph.D.-12 Phage Display Peptide Library. Further studies are needed to characterize the biological effects of these polypeptides and to explore the potential for these peptides to be used for the early screening of esophageal cancer or for cell-targeted therapies that would reduce the toxic side effects of cancer treatment.
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Two-dimensional monolayer cultures of human or murine cancer cell lines serve as preliminary models to evaluate phage targeting, transduction efficiency, and cytotoxic effects.
“Two-dimensional (2D) monolayer cultures of human or murine cancer cell lines serve as preliminary models to evaluate phage targeting, transduction efficiency, and cytotoxic effects [93,95,104,105]”
From Phage therapy in oncology: opportunities for cancer prevention and treatment (2026) · cites this paper as [105]
The Materials and methods section describes cultured human Eca109 cancer cells plated in wells, and the Results section reports testing phage binding to those cells. The paper does not report murine cancer cell cultures or measurements of transduction efficiency or cytotoxic effects. Page numbers are not available in our copy. (LLM, conf=0.88)
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Phage therapy in oncology: opportunities for cancer prevention and treatment
Mutalik, V. K., Inman, J. L., Chang, H., Arkin, A., & Mao, J.-H. (2026) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2026.02.001 · Cited by 5 · see citing works
- Authors
- Zhang, Z.-F., Shan, X., Wang, Y.-X., Wang, W., Feng, S.-Y., & Cui, Y.-B.
- Affiliations
- First Hospital of Jilin University (CN)
- Venue
- Journal of Cardiothoracic Surgery
- Published
- 2014-04-29
- Volume
- 9
- Issue
- 1
- Pages
- 76-76
- ISSN
- 1749-8090 (ISSN-L)
- PMC
- PMC4018990
- OpenAlex
- W2164549857
- Cited by
- 16
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Cardiothoracic Surgery (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Jilin University
- First Hospital of Jilin University
- Topics
- Health Sciences › Medicine › Surgery › Esophageal Cancer Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Keywords
- esophageal cancer, peptide ligands, phage display, tumor targeting, early cancer screening, tumor cell binding
- MeSH terms
- DNA, Neoplasm, Enzyme-Linked Immunosorbent Assay, Epithelial Cells, Esophageal Neoplasms, Genetic Testing, Humans, Immunohistochemistry, Peptides, Biomarkers, Tumor, Reproducibility of Results, Sequence Analysis, DNA, Peptide Library, Cell Line, Tumor, Cell Surface Display Techniques
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