Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
Abstract
Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.
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The well-established approach of (bacterio)phage display, developed in the 1980s, can be regarded as an in vitro MAVE prototype.
“The well-established approach of (bacterio)phage display, developed in the 1980s, can be regarded as an in vitro MAVE prototype (60, 61).”
From Massively parallel functional genomic assays in endocrinology: from promise to delivery (2026) · cites this paper as [60]
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- Authors
- Smith, G. P.
- Affiliations
- University of Missouri (US)
- Corresponding author
- Smith, George P.
- Venue
- Science
- Published
- 1985-06-14
- Volume
- 228
- Issue
- 4705
- Pages
- 1315-1317
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2070360032
- Cited by
- 4,143
- References
- 0
- Citations by year
- 2026: 129 · 2025: 145 · 2024: 158 · 2023: 180 · 2022: 188 · 2021: 183 · 2020: 168 · 2019: 172 · 2018: 151 · 2017: 159 · 2016: 166 · 2015: 151 · 2014: 174 · 2013: 166 · 2012: 165
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM18305)
- National Institute of Allergy and Infectious Diseases (AI20564)
- Topics
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- filamentous phage, antigen display, recombinant expression vector, gene cloning
- MeSH terms
- Antibodies, Viral, Antigens, Surface, Antigens, Viral, Cloning, Molecular, Coliphages, Genetic Vectors, Viral Proteins, Virion
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