Cited by 4,143Bacteriophages and microbial interactionsMonoclonal and Polyclonal Antibodies ResearchBacterial Genetics and Biotechnology

Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface

Smith, George P.·1985·Science

Abstract

Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.

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Crossref · 10.1126/science.4001944

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  • The well-established approach of (bacterio)phage display, developed in the 1980s, can be regarded as an in vitro MAVE prototype.

    “The well-established approach of (bacterio)phage display, developed in the 1980s, can be regarded as an in vitro MAVE prototype (60, 61).”

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Authors
Smith, G. P.
Affiliations
University of Missouri (US)
Corresponding author
Smith, George P.
Venue
Science
Published
1985-06-14
Volume
228
Issue
4705
Pages
1315-1317
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2070360032
Cited by
4,143
References
0
Citations by year
2026: 129 · 2025: 145 · 2024: 158 · 2023: 180 · 2022: 188 · 2021: 183 · 2020: 168 · 2019: 172 · 2018: 151 · 2017: 159 · 2016: 166 · 2015: 151 · 2014: 174 · 2013: 166 · 2012: 165
FWCI
4.02
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of General Medical Sciences (GM18305)
  • National Institute of Allergy and Infectious Diseases (AI20564)
Topics
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
filamentous phage, antigen display, recombinant expression vector, gene cloning
MeSH terms
Antibodies, Viral, Antigens, Surface, Antigens, Viral, Cloning, Molecular, Coliphages, Genetic Vectors, Viral Proteins, Virion

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