CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression
Open accessAbstract
BackroundCAP (CEVEC's Amniocyte Production) cells are an immortalized cell line based on primary human amniocytes.They were generated by transfection of these primary cells with a vector containing the functions E1 and pIX of adenovirus 5. CAP cells allow for competitive stable production of recombinant proteins with excellent biologic activity and therapeutic efficacy as a result of authentic human posttranslational modification.In order to gain access to the benefits of the CAP technology also for early research, target evaluation or assay development, the transient expression system CAP-T was developed.CAP-T cells are based on the original CAP cells and additionally express the large T antigen of simian virus 40 (SV40).
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CAP and HuH-7 cells display human-like glycosylation profiles.
“Two other cell lines, the CAP (CEVEC's Amniocyte Production) cells of human amniocytes origin and the HuH-7 cells of human hepatocellular carcinoma origin, are presently tested for recombinant glycoprotein production in preclinical phases and both display human-like glycosylation profiles (Schiedner et al., 2008;Wolfel et al., 2011;Enjolras et al., 2012;Swiech et al., 2012;Wissing et al., 2015).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Wolfel et al., 2011
The Summary (page not provided) says proteins produced by CAP-T cells showed a fully human posttranslational modification pattern, also observed in the original CAP cells. The Background describes CAP cells as producing proteins with authentic human posttranslational modifications. These passages support the claim for CAP cells, but the paper does not address HuH-7 cells or their glycosylation profiles. (LLM, conf=0.88)
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Research works printed but not linked: 1
- Essers R Kewes H Schiedner S Improving volumetric productivity of a stable human CAP cell line by bioprocess optimization BMC Proceedings abstract within the same supplement PMC3284939 22373410
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Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Wölfel, J., Essers, R., Bialek, C., Hertel, S., Scholz-Neumann, N., & Schiedner, G.
- Affiliations
- Federal Office of Administration (DE)
- Corresponding author
- Wölfel, Jens
- Venue
- BMC Proceedings
- Published
- 2011-11-22
- Volume
- 5
- Issue
- S8
- Pages
- P133-P133
- ISSN
- 1753-6561 (ISSN-L)
- PMC
- PMC3284976
- OpenAlex
- W2022729316
- Cited by
- 10
- References
- 0
- Citations by year
- 2026: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Proceedings (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- cap cells, transient protein expression, adenovirus type 5, SV40 large T antigen, recombinant protein expression, post-translational modification
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