Inhibition of HIV and HSV infection by vaginal lactobacilli in vitro and in vivo
Open accessAbstract
BACKGROUND AND THE PURPOSE OF THE STUDY: The cervico-vaginal mucosa which is populated with microflora (mostly includes lactobacilli) is the portal of entry for sexually transmitted pathogens. METHODS: The in vitro anti-viral effect of vaginal and non-vaginal lactobacillus was evaluated using single cycle HIV-1 replication and HSV-2 plaque reduction assays. The XTT proliferation assay was used to monitor the cellular toxicity. The in vivo anti-HSV-1 activity was evaluated in BALB/c mouse model by monitoring skin lesion and immune response development. RESULTS AND MAJOR CONCLUSION: DMEM culture supernatant of L. Gasseri and L. fermentum (PH 7.3) did not show toxic effect but inhibited 50% of HIV replication at 12 and 31% concentrations, respectively. Co-culture of L. gasseri (1000 CFU/ target cell) showed mild cytotoxicity but inhibited 68% of HIV replication. The supernatant of L. crispatus inhibited 50% of HSV replication at 4% and also co-culture of L. gasseri, L. rhamnosus and L. crispatus revokes almost all of the HSV multiplication. Culture supernatants of L. gasseri and L. crispatus had significant virucidal effect against the HIV and HSV and inhibited HSV infection in a stage before viral entry to the target cells. Alive L. gasseri cells showed high potential for inhibiting HSV-1 infection in vivo condition. Current data indicates that lactobacilli supernatant encompasses components with neutralizing activity against HIV and HSV and it would be a determinant factor for viral diseases transmission and promising lead for anti-viral probiotic design.
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Direct virucidal effects have been observed for selected probiotic lactobacilli, with primary metabolites such as hydrogen peroxide or lowering pH by organic acids having such effects.
“Direct virucidal effects have been observed for selected probiotic lactobacilli, primary metabolites such as hydrogen peroxide or lowering the pH by organic acids have such effects (Zabihollahi et al., 2012).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Zabihollahi et al., 2012
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- Authors
- Zabihollahi, R., Motevaseli, E., Sadat, S. M., Azizi-Saraji, A. R., Asaadi-Dalaie, S., & Modarressi, M. H.
- Affiliations
- Pasteur Institute of Iran (IR)
- Tehran University of Medical Sciences (IR)
- Corresponding author
- Modarressi, Mohammad Hossein
- Venue
- DARU Journal of Pharmaceutical Sciences
- Published
- 2012-10-15
- Volume
- 20
- Issue
- 1
- Pages
- 53-53
- ISSN
- 1560-8115 (ISSN-L)
- 2008-2231
- PMC
- PMC3555973
- OpenAlex
- W2168058236
- Cited by
- 39
- References
- 28
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 8 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 8 · 2015: 3
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- DARU Journal of Pharmaceutical Sciences (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Keywords
- vaginal lactobacilli, HIV-1 inhibition, herpes simplex virus type 2, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus rhamnosus, probiotics, antiviral activity, vaginal microbiota, viral entry inhibition, skin lesions, mouse model
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