The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Metagenomics - a guide from sampling to data analysis
Open accessAbstract
Metagenomics applies a suite of genomic technologies and bioinformatics tools to directly access the genetic content of entire communities of organisms. The field of metagenomics has been responsible for substantial advances in microbial ecology, evolution, and diversity over the past 5 to 10 years, and many research laboratories are actively engaged in it now. With the growing numbers of activities also comes a plethora of methodological knowledge and expertise that should guide future developments in the field. This review summarizes the current opinions in metagenomics, and provides practical guidance and advice on sample processing, sequencing technology, assembly, binning, annotation, experimental design, statistical analysis, data storage, and data sharing. As more metagenomic datasets are generated, the availability of standardized procedures and shared data storage and analysis becomes increasingly important to ensure that output of individual projects can be assessed and compared.
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Cites
A human gut microbial gene catalogue established by metagenomic sequencing
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<tt>BLAT</tt> —The <tt>BLAST</tt> -Like Alignment Tool
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Cited by
Next-Generation Sequencing Technology: Current Trends and Advancements
Satam, H., Joshi, K., Mangrolia, U., Waghoo, S., Zaidi, G., Rawool, S., Thakare, R. P., Banday, S., Mishra, A. K., Das, G., et al. (2023) · Biology · doi:10.3390/biology12070997 · Cited by 1,299
Critical Assessment of Metagenome Interpretation—a benchmark of metagenomics software
Sczyrba, A., Hofmann, P., Belmann, P., Koslicki, D., Janssen, S., Dröge, J., Gregor, I., Majda, S., Fiedler, J., Dahms, E., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4458 · Cited by 870
Self-citationPhyloSift: phylogenetic analysis of genomes and metagenomes
Darling, A. E., Jospin, G., Lowe, E., Matsen, F. A., Bik, H. M., & Eisen, J. A. (2014) · PeerJ · doi:10.7717/peerj.243 · Cited by 631
Current challenges and best-practice protocols for microbiome analysis
Bharti, R., & Grimm, D. G. (2021) · Briefings in Bioinformatics · doi:10.1093/bib/bbz155 · Cited by 592
Oulas, A., Pavloudi, C., Polymenakou, P., Pavlopoulos, G. A., Papanikolaou, N., Kotoulas, G., Arvanitidis, C., & Iliopoulos, L. (2015) · Bioinformatics and Biology Insights · doi:10.4137/bbi.s12462 · Cited by 416
Metadata
- Authors
- Thomas, T., Gilbert, J., & Meyer, F.
- Venue
- Microbial Informatics and Experimentation
- Published
- 2012-02-09
- Volume
- 2
- Issue
- 1
- Pages
- 3-3
- ISSN
- 2042-5783 (ISSN-L)
- OpenAlex
- W2120267228
- Cited by
- 1,029
- References
- 10
- FWCI
- 22.54
- Type
- journal article
- Language
- en
- Open access
- hybrid
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