The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice
Open accessAbstract
Background: The type II clustered, regularly interspaced, short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) system is a novel molecular tool for site-specific genome modification. The CRISPR-Cas9 system was recently introduced into plants by transient or stable transformation. Findings: Here, we report gene targeting in rice via the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system. Three 20-nt CRISPR RNAs were designed to pair with diverse sites followed by the protospacer adjacent motif (PAM) of the rice herbicide resistance gene BEL. After integrating the single-guide RNA (sgRNA) and Cas9 cassette in a single binary vector, transgenic rice plants harboring sgRNA:Cas9 were generated by A. tumefaciens-mediated stable transformation. By analyzing the targeting site on the genome of corresponding transgenic plants, the mutations were determined. The mutagenesis efficiency was varied from ~2% to ~16%. Furthermore, phenotypic analysis revealed that the biallelic mutated transgenic plant was sensitive to bentazon. Conclusions: Our results indicate that the agricultural trait could be purposely modified by sgRNA:Cas9-induced gene targeting. CRISPR-Cas9 system could be exploited as a powerful tool for trait improvements in crop breeding.
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Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.04.025 · Cited by 3,495
Cited by
Wang, F., Wang, C., Liu, P., Lei, C., Hao, W., Gao, Y., Liu, Y.-G., & Zhao, K. (2016) · PLOS ONE · doi:10.1371/journal.pone.0154027 · Cited by 819
Editing plant genomes with CRISPR/Cas9
Belhaj, K., Chaparro-Garcia, A., Kamoun, S., Patron, N. J., & Nekrasov, V. (2015) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.11.007 · Cited by 537
Pan, C., Ye, L., Qin, L., Liu, X., He, Y., Wang, J., Chen, L., & Lu, G. (2016) · Scientific Reports · doi:10.1038/srep24765 · Cited by 375
Enhancing genetic gain in the era of molecular breeding
Xu, Y., Li, P., Zou, C., Lu, Y., Xie, C., Zhang, X., Prasanna, B. M., & Olsen, M. S. (2017) · Journal of Experimental Botany · doi:10.1093/jxb/erx135 · Cited by 336
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
Mikami, M., Toki, S., & Endo, M. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0342-x · Cited by 296
Metadata
- Authors
- Xu, R., Li, H., Qin, R., Wang, L., Li, L., Wei, P., & Yang, J.
- Venue
- Rice
- Published
- 2014-05-02
- Volume
- 7
- Issue
- 1
- Pages
- 5-5
- ISSN
- 1939-8425 (ISSN-L)
- 1939-8433
- OpenAlex
- W2129993269
- Cited by
- 227
- References
- 4
- FWCI
- 8.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Xu, R., Li, H., Qin, R., Wang, L., Li, L., Wei, P., & Yang, J. (2014). Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice. Rice. https://doi.org/10.1186/s12284-014-0005-6
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