The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations
Open accessAbstract
The CRISPR/Cas9 system has successfully been used in various organisms for precise targeted gene editing. Although it has been demonstrated that CRISPR/Cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the CRISPR/Cas9 system in tomato plants have not yet been elucidated in detail. In this study, two genes, SlPDS and SlPIF4, were used for testing targeted mutagenesis in tomato plants through an Agrobacterium tumefaciens-mediated transformation method. A high mutation frequency was observed in all tested targets in the T0 transgenic tomato plants, with an average frequency of 83.56%. Clear albino phenotypes were observed for the psd mutants. High frequencies of homozygous and biallelic mutants were detected even in T0 plants. The majority of the detected mutations were 1- to 3-nucleotide deletions, followed by 1-bp insertions. The target mutations in the T0 lines were stably transmitted to the T1 and T2 generations, without new modifications or revision. Off-target activities associated with SlPDS and SlPIF4 were also evaluated by sequencing the putative off-target sites and no clear off-target events were detected. Our results demonstrate that the CRISPR/Cas9 system is an efficient tool for generating stable and heritable modifications in tomato plants.
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Cites
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Cited by
Peng, A., Chen, S., Lei, T., Xu, L., He, Y., Wu, L., Yao, L., & Zou, X. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12733 · Cited by 731
Self-citationCRISPR for Crop Improvement: An Update Review
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 619
CRISPR/Cas system: recent advances and future prospects for genome editing
Manghwar, H., Lindsey, K., Zhang, X., & Jin, S. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.09.006 · Cited by 529
“basic helix-loophelix multigene family [133]”
CRISPR/Cas9‐mediated efficient targeted mutagenesis in grape in the first generation
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Gene Editing and Crop Improvement Using CRISPR-Cas9 System
Arora, L., & Narula, A. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01932 · Cited by 367
Metadata
- Authors
- Pan, C., Ye, L., Qin, L., Liu, X., He, Y., Wang, J., Chen, L., & Lu, G.
- Venue
- Scientific Reports
- Published
- 2016-04-21
- Volume
- 6
- Issue
- 1
- Pages
- 24765-24765
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2336717918
- Cited by
- 375
- References
- 57
- FWCI
- 18.06
- Type
- journal article
- Language
- en
- Open access
- gold
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