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Cited by 26Clostridium difficile and Clostridium perfringens researchBacterial Identification and Susceptibility TestingGenomics and Phylogenetic Studies

Comparative genomic and phenomic analysis of Clostridium difficile and Clostridium sordellii, two related pathogens with differing host tissue preference.

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Scaria, Joy, Suzuki, Haruo, Ptak, Christopher P., Chen, Jenn-Wei, Zhu, Yongzhang, Guo, Xiao-Kui, Chang, Yung-Fu·2015·BMC Genomics
DOI 10.1186/s12864-015-1663-5PMC PMC4462011
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Abstract

BACKGROUND: Clostridium difficile and C. sordellii are two anaerobic, spore forming, gram positive pathogens with a broad host range and the ability to cause lethal infections. Despite strong similarities between the two Clostridial strains, differences in their host tissue preference place C. difficile infections in the gastrointestinal tract and C. sordellii infections in soft tissues. RESULTS: In this study, to improve our understanding of C. sordellii and C. difficile virulence and pathogenesis, we have performed a comparative genomic and phenomic analysis of the two. The global phenomes of C. difficile and C. sordellii were compared using Biolog Phenotype microarrays. When compared to C. difficile, C. sordellii was found to better utilize more complex sources of carbon and nitrogen, including peptides. Phenotype microarray comparison also revealed that C. sordellii was better able to grow in acidic pH conditions. Using next generation sequencing technology, we determined the draft genome of C. sordellii strain 8483 and performed comparative genome analysis with C. difficile and other Clostridial genomes. Comparative genome analysis revealed the presence of several enzymes, including the urease gene cluster, specific to the C. sordellii genome that confer the ability of expanded peptide utilization and survival in acidic pH. CONCLUSIONS: The identified phenotypes of C. sordellii might be important in causing wound and vaginal infections respectively. Proteins involved in the metabolic differences between C. sordellii and C. difficile should be targets for further studies aimed at understanding C. difficile and C. sordellii infection site specificity and pathogenesis.

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  • The chemical similarity between C. difficile toxin A and P. sordellii hemorrhagic toxin has been identified and immunological cross-reactivity recognized, with both species 71% homologous at the genomic level.

    “The chemical similarity between C. difficile toxin A and P. sordellii hemorrhagic toxin has been identified and immunological cross-reactivity recognized, with both species 71% homologous at the genomic level (Martinez and Wilkins, 1988;Scaria et al., 2015).”

    From Novel Lethal Clostridial Infection in Florida Manatees (Trichechus manatus latirostris): Cause of the 2013 Unusual Mortality Event in the Indian River Lagoon (2022) · cites this paper as Scaria et al., 2015

    The Background reports immunological cross-reactivity between toxins of C. difficile and C. sordellii. The Results and discussion section on gene repertoire comparison reports homologous toxin proteins, including a C. sordellii hemorrhagic toxin, but does not establish the claimed chemical similarity between the specific toxins or state that the species are 71% homologous at the genomic level. Page numbers are unavailable in our copy. (LLM, conf=0.87)

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Authors
Scaria, J., Suzuki, H., Ptak, C. P., Chen, J.-W., Zhu, Y., Guo, X.-K., & Chang, Y.-F.
Affiliations
  • South Dakota State University (US)
  • Yamaguchi University (JP)
  • Cornell University (US)
  • Shanghai Jiao Tong University (CN)
Corresponding author
Chang, Yung-Fu
ORCID
Venue
BMC Genomics
Publisher
Springer Science and Business Media LLC
Published
2015-06-10
Volume
16
Issue
1
Pages
448-448
ISSN
1471-2164 (ISSN-L)
DOI
10.1186/s12864-015-1663-5
PMC
PMC4462011
OpenAlex
W1560169010
Cited by
26
References
2
Citations by year
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FWCI
1.82
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Genomics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Health and Human Services
  • U.S. Department of Agriculture
  • National Institutes of Health (n01ai30054-10-0-2)
  • National Institute of Allergy and Infectious Diseases (N01 AI030054, N01AI30054)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
Clostridium difficile, Clostridium sordellii, comparative genomics, phenotypic profiling, peptide utilization, acid resistance, urease gene cluster, draft genome sequence, wound infection, vaginal infection, pathogenesis, virulence
MeSH terms
Hydrogen-Ion Concentration, Phenotype, Phylogeny, Clostridioides difficile, Genome, Bacterial, Sequence Analysis, DNA, Genomics, Clostridium sordellii, Paraclostridium sordellii, Host Specificity, High-Throughput Nucleotide Sequencing

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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