Comprehensive analyses of long non-coding RNA expression profiles by RNA sequencing and exploration of their potency as biomarkers in psoriatic arthritis patients
Open accessAbstract
BACKGROUND: The aim of the current study was to investigate the long non-coding RNA (lncRNA) expression profiles in psoriatic arthritis (PSA) patients by RNA sequencing, and to further explore potential biomarkers that were able to predict PSA risk and activity. METHODS: LncRNA and mRNA expression profiles in peripheral blood mononuclear cells (PBMC) of 4 PSA patients and 4 normal controls (NCs) were detected by RNA sequencing, followed by comprehensive bioinformatic analyses. Subsequently, 3 top upregulated and 2 top downregulated lncRNAs were chosen for further validation in 93 PSA patients and 93 NCs by quantitative polymerase chain reaction (qPCR) assay. RESULTS: Totally 76 upregulated and 54 downregulated lncRNAs, as well as 231 upregulated and 102 downregulated mRNAs were discovered in PSA patients compared with NCs. Enrichment analyses revealed that they were mostly associated with nucleosome, extracellular exosome and extracellular matrix, and the top enriched pathways were systemic lupus erythematosus (SLE), alcoholism and viral carcinogenesis. qPCR assay showed that lnc-RP11-701H24.7 and lnc-RNU12 were upregulated in PSA patients compared with NCs, and they could predict PSA risk with high area under curves. Besides, lnc-RP11-701H24.7 was positively associated with ESR, SJC, TJC and pain VAS score while lnc-RNU12 was positively correlated with PASI score, CRP and PGA score, implying that both of them were positively correlated with disease activity. CONCLUSION: Our study facilitates comprehensive understanding of lncRNA expression profiles in PSA pathogenesis, and discovers that lnc-RP11-701H24.7 and lnc-RNU12 might be served as novel biomarkers for PSA risk and activity.
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Transport of ncRNA (including miRNA and lncRNA) target cells through exosomes plays a role in the pathophysiology of psoriatic arthritis.
“For example, recent evidence suggests that the transport of ncRNA (including miRNA and lncRNA) target cells through the role of exosomes in the pathophysiology of psoriatic arthritis [104].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [104]
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The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System
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- Authors
- Yue, T., Ji, M., Qu, H., Guo, M., Bai, F., Zhang, Z., Wang, W., Gong, X., & Zhang, Z.
- Affiliations
- Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine (CN)
- Shanghai Skin Disease Hospital (CN)
- Shanghai University of Traditional Chinese Medicine (CN)
- Fudan University (CN)
- Corresponding author
- Yue, Tao
- Zhang, Zhanming
- Zhang, Zhenghua
- Venue
- BMC Immunology
- Published
- 2019-08-07
- Volume
- 20
- Issue
- 1
- Pages
- 28-28
- ISSN
- 1471-2172 (ISSN-L)
- PMC
- PMC6686418
- OpenAlex
- W2964655141
- Cited by
- 19
- References
- 34
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 2 · 2022: 7 · 2021: 2 · 2020: 4 · 2019: 1
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Immunology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Prioyity Speciality for Changning District, Shanghai Municipal Commission of Health and Family Planning (20161003)
- Topics
- Health Sciences › Medicine › Rheumatology › Spondyloarthritis Studies and Treatments
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- psoriatic arthritis, long non-coding RNA, lncRNA expression profiles, biomarkers, disease activity, disease risk prediction, peripheral blood mononuclear cells, exosomes, extracellular matrix
- MeSH terms
- Female, Humans, Male, RNA, Messenger, Sensitivity and Specificity, Biomarkers, Arthritis, Psoriatic, Computational Biology, Gene Expression Profiling, Gene Regulatory Networks, Workflow, High-Throughput Nucleotide Sequencing, Transcriptome, RNA, Long Noncoding, Gene Ontology
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