Comparing Various Protocols of Human and Bovine Ovarian Tissue Decellularization to Prepare Extracellular Matrix-Alginate Scaffold for Better Follicle Development In Vitro
Open accessAbstract
BACKGROUND: Nowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, different fertility preservation techniques were developed. Artificial ovary is one of these methods suggested by several scientific groups. Decellularized ovarian cortex has been introduced as a scaffold in the field of human fertility preservation. This study was carried out to compare decellularization of the ovarian scaffold by various protocols and evaluate the follicle survival in extracellular matrix (ECM)-alginate scaffold. RESULTS: The micrographs of H&E and DAPI staining confirmed successful decellularization of the ovarian cortex in all experimental groups, but residual DNA content in SDS-Triton group was significantly higher than other groups (P < 0.05). SEM images demonstrated that complex fiber network and porosity structure were maintained in all groups. Furthermore, elastin and collagen fibers were observed in all groups after decellularization process. MTT test revealed higher cytobiocompatibility of the SDS-Triton-Ammonium and SDS-Triton decellularized scaffolds compared with SDS groups. Compared to the transferred follicles into the sodium alginate (81%), 85.9% of the transferred follicles into the decellularized scaffold were viable after 7 days of cultivation (P = 0.04). CONCLUSION: Although all the decellularization procedures was effective in removal of cells from ovarian cortex, SDS-Triton-Ammonium group showed less residual DNA content with higher cytobiocompatibility for follicles when compared with other groups. In addition, the scaffold made from ovarian tissues decellularized using SDS-Triton-Ammonium and sodium alginate is suggested as a potential 3D substrate for in vitro culture of follicles for fertility preservation.
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A mixture of sodium alginate with decellularized murine ovarian tissue supported in vitro follicle survival.
“A mixture of sodium alginate with decellularized murine ovarian tissue supported in vitro follicle survival (Nikniaz et al., 2021).”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Nikniaz et al., 2021
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- Authors
- Nikniaz, H., Zandieh, Z., Nouri, M., Daei-farshbaf, N., Aflatoonian, R., Gholipourmalekabadi, M., & Jameie, S. B.
- Affiliations
- Iran University of Medical Sciences (IR)
- Tabriz University of Medical Sciences (IR)
- Royan Institute (IR)
- Corresponding author
- Zandieh, Zahra
- Jameie, Seyed Behnamedin
- ORCID
- Venue
- BMC Biotechnology
- Published
- 2021-01-20
- Volume
- 21
- Issue
- 1
- Pages
- 8-8
- ISSN
- 1472-6750 (ISSN-L)
- PMC
- PMC7816475
- OpenAlex
- W3124359203
- Cited by
- 55
- References
- 27
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 17 · 2023: 11 · 2022: 10 · 2021: 3
- FWCI
- 9.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Biotechnology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Iran University of Medical Sciences
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- artificial ovary, female fertility preservation, ovarian cortex, folliculogenesis, 3D scaffolds, cancer survivors
- MeSH terms
- Adult, Alginates, Animals, Biocompatible Materials, Cattle, Extracellular Matrix, Female, Ovarian Follicle, Humans, Middle Aged, Ovary, Hydrogels, Tissue Engineering, Mice, Tissue Scaffolds, Fertility Preservation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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