CoChat
Sign Up
Cited by 8Mesenchymal stem cell researchUltrasound and Hyperthermia ApplicationsLiver physiology and pathology

Ultrasonic microbubbles promote mesenchymal stem cell homing to the fibrotic liver via upregulation of CXCR4 expression

Open access
Xu, Heming, Huang, Yize, Zhang, Fasu, Shi, Wei, Cheng, Yan, Yang, Kai, Tian, Pingping, Zhou, Fei +5 more·2024·Cell Division
DOI 10.1186/s13008-023-00104-8PMC PMC10894490
Open this paper in CoChat — free accountOpen PDF

Abstract

Objective: To investigate the mechanism of ultrasound microbubbles (UTMB) promoting stem cells homing to fibrotic liver. Methods: Bone marrow derived mesenchymal stem cells (BMSCs) were divided into 5 groups with or without ultrasound microbubbles and continuously irradiated with ultrasound conditions of frequency 1 MHZ and output power 0.6 W/cm 2 for different times, and then injected into a mouse model of liver fibrosis through the tail vein with or without ultrasound microbubbles, with sound intensity. The effect of ultrasound microbubbles on MSC expression of CXC chemokine receptor 4 (CXCR4) and homing fibrotic liver was evaluated by flow cytometry (FCM), western blot (WB) and immunohistochemistry (IHC) analysis. Results: The level of CXCR4 expression was significantly higher in the ultrasound microbubble group than in the non-intervention group (P < 0.05), and the number of MSC and the rate of CXCR4 receptor positivity in the ultrasound microbubble-treated liver tissues were significantly higher than in the non-intervention group (P < 0.01). Conclusion: Ultrasonic microbubbles can promote the expression of CXCR4 on the surface of MSCs, thus improving the homing rate of MSCs in fibrotic liver.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/s13008-023-00104-8

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 24

  1. [1]Zhang Y Li Y Zhang L Mesenchymal stem cells: potential application for the treatment of hepatic cirrhosis Stem Cell Res Ther 2018 9 1 59 10.1186/s13287-018-0814-4 29523186 PMC5845383 · DOI
  2. [2]Terai S Tsuchiya A Status of and candidates for cell therapy in liver cirrhosis: overcoming the "point of no return" in advanced liver cirrhosis J Gastroenterol 2017 52 2 129 140 10.1007/s00535-016-1258-1 27631592 · DOI
  3. [3]Rong X Liu J Yao X Human bone marrow mesenchymal stem cells-derived exosomes alleviate liver fibrosis through the Wnt/β-catenin pathway Stem Cell Res Ther 2019 10 1 98 106 10.1186/s13287-019-1204-2 30885249 PMC6421647 · DOI
  4. [4]Fang XQ Liu LW Dong J A study about immunomodulatory effect and efficacy and prognosis of human umbilical cord mesenchymal stem cells in patients with chronic hepatitis B-induced decompensated liver cirrhosis J Gastroenterol Hepatol 2018 33 4 774 780 10.1111/jgh.14081 29293276 · DOI · Paper page
  5. [6]Ammar HI Shamseldeen AM Shoukry HS Metformin impairs homing ability and efficacy of mesenchymal stem cells for cardiac repair in streptozotocin-induced diabetic cardiomyopathy in rats Am J Physiol Heart Circ Physiol 2021 320 4 H1290 H1302 10.1152/ajpheart.00317.2020 33513084 · DOI
  6. [7]Jiang Q Huang K Lu F Modifying strategies for SDF-1/CXCR4 interaction during mesenchymal stem cell transplantation Gen Thorac Cardiovasc Surg 2022 70 1 1 10 10.1007/s11748-021-01696-0 34510332 PMC8732940 · DOI
  7. [8]Vitale E Rossin D Perveen S Silica nanoparticle internalization improves chemotactic behaviour of human mesenchymal stem cells acting on the SDF1α/CXCR4 axis Biomedicines 2022 10 2 336 10.3390/biomedicines10020336 35203545 PMC8961775 · DOI
  8. [9]Bidkhori HR Bahrami AR Farshchian M Mesenchymal stem/stromal cells overexpressing CXCR4R334X revealed enhanced migration: a lesson learned from the pathogenesis of WHIM syndrome Cell Transp 2021 30 9636897211054498 10.1177/09636897211054498 PMC8647223 34807749 · DOI
  9. [10]Zheng J Li H He L Preconditioning of umbilical cord-derived mesenchymal stem cells by rapamycin increases cell migration and ameliorates liver ischaemia/reperfusion injury in mice via the CXCR4/CXCL12 axis Cell Prolif 2019 52 2 e12546 10.1111/cpr.12546 30537044 PMC6496237 · DOI
  10. [11]Potter Matthew L Kathryn S Characterization of differentially expressed miRNAs by CXCL12/SDF-1 in human bone marrow stromal cells Biomol Concepts 2021 12 132 143 10.1515/bmc-2021-0015 34648701 · DOI
  11. [12]Chen L Luo W Wang Y Directional homing of glycosylation-modified bone marrow mesenchymal stem cells for bone defect repair J Nanobiotechnol 2021 19 1 228 10.1186/s12951-021-00969-3 PMC8325817 34332597 · DOI
  12. [13]Szydlak R Biological, chemical and mechanical factors regulating migration and homing of mesenchymal stem cells World J Stem Cells 2021 13 6 619 631 10.4252/wjsc.v13.i6.619 34249231 PMC8246245 · DOI
  13. [14]Le DQ Papadopoulou V Dayton PA Effect of acoustic parameters and microbubble concentration on the likelihood of encapsulated microbubble coalescence Ultrasound Med Biol 2021 47 10 2980 2989 10.1016/j.ultrasmedbio.2021.06.020 34344561 PMC8547186 · DOI
  14. [15]Li L Wu S Liu Z Ultrasound-targeted microbubble destruction improves the migration and homing of mesenchymal stem cells after myocardial infarction by upregulating SDF-1/CXCR4: a pilot study Stem Cells Int 2015 2015 691310 10.1155/2015/691310 26074977 PMC4436519 · DOI
  15. [16]Ye F Zhai M Long J The burden of liver cirrhosis in mortality: Results from the global burden of disease study Front Public Health 2022 10 909455 10.3389/fpubh.2022.909455 36033800 PMC9403789 · DOI
  16. [17]Kot M Musiał-Wysocka A Lasota M Secretion, migration and adhesion as key processes in the therapeutic activity of mesenchymal stem cells Acta Biochim Pol 2019 66 4 499 507 31883363
  17. [18]Li Y Du M Fang J UTMB promoted local delivery of miR-34a-mimic for ovarian cancer therapy Drug Deliv 2021 28 1 1616 1625 10.1080/10717544.2021.1955041 34319204 PMC8330777 · DOI
  18. [19]Wei X Zheng Y Zhang W Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats Int J Mol Med 2021 47 2 677 687 10.3892/ijmm.2020.4830 33416139 PMC7797467 · DOI
  19. [20]Chowdhury SM Abou-Elkacem L Lee T Ultrasound and microbubble mediated therapeutic delivery: underlying mechanisms and future outlook J Control Release 2020 326 75 90 10.1016/j.jconrel.2020.06.008 32554041 · DOI
  20. [21]Wang G Zhang Q Zhuo Z Effects of diagnostic ultrasound-targeted microbubet ble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma Eur Radiol 2016 26 9 3006 3016 10.1007/s00330-015-4123-3 26637447 · DOI
  21. [22]Su GF Liu LY Yang LJ Homing of endogenous bone marrow mesenchymal stem cells to rat infarcted myocardium via ultrasound-mediated recombinant SDF-1α adenovirus in microbubbles Oncotarget 2018 9 1 477 487 10.18632/oncotarget.23068 29416629 PMC5787482 · DOI
  22. [23]Wang G Zhang Q Zhuo Z Enhanced homing of CXCR-4 modified bone marrow-derived mesenchymal stem cells to acute kidney injury tissues by micro-bubble-mediated ultrasound exposure Ultrasound Med Biol 2016 42 2 539 548 10.1016/j.ultrasmedbio.2015.10.005 26610714 · DOI
  23. [24]Cui H Zhu Q Xie Q Low intensity ultrasound targeted microbubble destruction assists MSCs delivery and improves neural function in brain ischaemic rats J Drug Target 2020 28 3 320 329 10.1080/1061186X.2019.1656724 31429596 · DOI
  24. [25]Karlas T Franke GN Bedeutung der Lebersteifigkeitsmessung für Risikoprädiktion und Komplikationsmanagement bei allogener Stammzelltransplantation Z Gastroenterol 2021 59 9 995 996 10.1055/a-1538-6732 34507378 · DOI

Cited by

Sorted by citations

Authors
Xu, H., Huang, Y., Zhang, F., Shi, W., Cheng, Y., Yang, K., Tian, P., Zhou, F., Wang, Y., Fang, X., Song, Y., Liu, B., & Liu, L.
Affiliations
  • The People's Hospital Tongling (CN)
  • Anhui Medical University (CN)
Corresponding author
  • Liu, Bo
  • Liu, Liwei
ORCID
Venue
Cell Division
Publisher
Springer Science and Business Media LLC
Published
2024-02-24
Volume
19
Issue
1
Pages
7-7
ISSN
1747-1028 (ISSN-L)
DOI
10.1186/s13008-023-00104-8
PMC
PMC10894490
OpenAlex
W4392139151
Cited by
8
References
1
Citations by year
2026: 3 · 2025: 4 · 2024: 1
FWCI
2.25
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Division (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Anhui Medical College ((WJH2022006t))
  • Scientific research project of Anhui Universities ((2022AH052332))
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
ultrasound microbubbles, mesenchymal stem cells, stem cell homing, liver fibrosis, CXCR4, bone marrow-derived stem cells, CXCR4 expression

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review