The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit
Open accessAbstract
BACKGROUND: Bone fractures are medical emergencies that require prompt intervention to help return bone to its normal function. Various methods and treatments have been utilized to increase the speed and efficiency of bone repair. This study aimed to investigate the treatment effects of Prunus dulcis aqueous extract on tibial bone healing in rabbits. METHODS: doses of Prunus dulcis extract, and osteocare treated group. Biochemical blood factors including calcium, phosphorus, and alkaline phosphatase (on days 0, 10, 30, and 50), biomarkers of oxidative stress such as GPx, CAT, and MDA (on days 10 and 30), radiological evaluation, histopathological parameters, and osteocalcin immunohistochemical expression were assessed. RESULTS: The data showed calcium levels in the treatment groups increased significantly from day 10 to day 50, respectively, and blood phosphorus levels decreased from day 10 to day 50 in the treatment groups. Alkaline phosphatase initially increased and then decreased in treatment groups. In the treatment groups, GPx and CAT levels significantly increased, and the serum amount of MDA reduced. The best antioxidant results were related to the extract-treated group with a higher dose. Radiographic score was significantly higher in the treatment groups than the control group on day 30. Based on the pathological findings, the healing occurred faster in the extract-treated group with a higher dose. Osteocalcin expression was significantly higher in the control group than that in the treatment groups. CONCLUSIONS: Treatment with Prunus dulcis extract with a dosage of 300 mg/kg accelerated tibial bone healing in rabbits.
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For irisin, tumeric acid, aqueous extract of Prunus dulcis, and the Chinese herbal formula 'Zhèng Gŭ Zĭ Jīn Dān', acceleration of fracture healing has not yet been shown in clinical studies with patients and appropriate study design.
“Aditionally, irisin (119), tumeric acid (120), aqueous extract of Prunus dulcis (121), and the Chinese acupuncture and herbal formula powder prescription ‘Zhèng Gŭ Zĭ Jīn Dān’ (122) are thought to accelerate fracture healing, but this has not yet been shown in clinical studies with patients and with an appropriate study design.”
From Methods to accelerate fracture healing – a narrative review from a clinical perspective (2024) · cites this paper as [121]
The Abstract Conclusions report accelerated tibial bone healing with 300 mg/kg Prunus dulcis extract in rabbits, and the Methods confirm this was an animal study, not a clinical study with patients. The paper does not establish the broader claim that acceleration has not yet been shown in any appropriately designed clinical study. (LLM, conf=0.86)
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Methods to accelerate fracture healing – a narrative review from a clinical perspective
Ganse, B. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1384783 · Cited by 33 · see citing works
“Aditionally, irisin (119), tumeric acid (120), aqueous extract of Prunus dulcis (121), and the Chinese acupuncture and herbal formula powder prescription ‘ Zhèng Gu ̆ Z ĭ J ı ̄ n Da ̄ n ’ (122) are thought to accelerate fracture healing, but this has not yet been shown in clinical studies with…”
Background
- Authors
- Anaraki, N., Beyraghi, A. H., Raisi, A., Davoodi, F., Farjanikish, G., & Sadegh, A. B.
- Affiliations
- Lorestan University (IR)
- Shiraz University (IR)
- Corresponding author
- Raisi, Abbas
- Davoodi, Farshid
- Venue
- Journal of Orthopaedic Surgery and Research
- Published
- 2021-06-07
- Volume
- 16
- Issue
- 1
- Pages
- 362-362
- ISSN
- 1749-799X (ISSN-L)
- PMC
- PMC8183070
- OpenAlex
- W3171778743
- Cited by
- 10
- References
- 32
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 2
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Orthopaedic Surgery and Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- almond, aqueous plant extract, fracture healing, osteocalcin, oxidative stress, antioxidant enzymes, alkaline phosphatase, calcium and phosphorus levels, bone regeneration, herbal medicine
- MeSH terms
- Prunus dulcis, Animals, Antioxidants, Bone Regeneration, Male, Phytotherapy, Plant Extracts, Rabbits, Tibia, Tibial Fractures, Water, Wound Healing, Osteocalcin, Oxidative Stress
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