The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
CRISPR-Cas13d mediates robust RNA virus interference in plants
Open accessAbstract
Background: CRISPR-Cas systems endow bacterial and archaeal species with adaptive immunity mechanisms to fend off invading phages and foreign genetic elements. CRISPR-Cas9 has been harnessed to confer virus interference against DNA viruses in eukaryotes, including plants. In addition, CRISPR-Cas13 systems have been used to target RNA viruses and the transcriptome in mammalian and plant cells. Recently, CRISPR-Cas13a has been shown to confer modest interference against RNA viruses. Here, we characterized a set of different Cas13 variants to identify those with the most efficient, robust, and specific interference activities against RNA viruses in planta using Nicotiana benthamiana. Results: Our data show that LwaCas13a, PspCas13b, and CasRx variants mediate high interference activities against RNA viruses in transient assays. Moreover, CasRx mediated robust interference in both transient and stable overexpression assays when compared to the other variants tested. CasRx targets either one virus alone or two RNA viruses simultaneously, with robust interference efficiencies. In addition, CasRx exhibits strong specificity against the target virus and does not exhibit collateral activity in planta. Conclusions: Our data establish CasRx as the most robust Cas13 variant for RNA virus interference applications in planta and demonstrate its suitability for studying key questions relating to virus biology.
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Cites
RNA targeting with CRISPR–Cas13
Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T., Kellner, M. J., Regev, A., et al. (2017) · Nature · doi:10.1038/nature24049 · Cited by 2,093
Cited by
Applications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870
Massively parallel Cas13 screens reveal principles for guide RNA design
Wessels, H.-H., Méndez-Mancilla, A., Guo, X., Legut, M., Daniloski, Z., & Sanjana, N. E. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0456-9 · Cited by 418
Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies
Aman, R., Mahas, A., & Mahfouz, M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00507 · Cited by 409
Self-citationZaidi, S. S.-E.-A., Mahas, A., Vanderschuren, H., & Mahfouz, M. M. (2020) · Genome Biology · doi:10.1186/s13059-020-02204-y · Cited by 221
Self-citationTranscriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens
Guo, X., Rahman, J. A., Wessels, H.-H., Méndez-Mancilla, A., Haro, D., Chen, X., & Sanjana, N. E. (2021) · Cell Genomics · doi:10.1016/j.xgen.2021.100001 · Cited by 79
Metadata
- Authors
- Mahas, A., Aman, R., & Mahfouz, M.
- Venue
- Genome Biology
- Published
- 2019-12
- Volume
- 20
- Issue
- 1
- Pages
- 263-263
- ISSN
- 1465-6906 (ISSN-L)
- 1474-760X
- OpenAlex
- W2991671000
- Cited by
- 188
- References
- 1
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- gold
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