Applications of CRISPR–Cas in agriculture and plant biotechnology

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210 · see citing works

Global food demand and the sustainable intensification of agriculture

Tilman, D., Balzer, C., Hill, J., & Befort, B. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1116437108 · Cited by 7,692 · see citing works

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437 · see citing works

Search-and-replace genome editing without double-strand breaks or donor DNA

Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767 · see citing works

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184 · see citing works

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696 · see citing works

RNA targeting with CRISPR–Cas13

Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T., Kellner, M. J., Regev, A., et al. (2017) · Nature · doi:10.1038/nature24049 · Cited by 2,093 · see citing works

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., & Qiu, J.-L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2969 · Cited by 2,072 · see citing works

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

Christian, M. L., Čermák, T., Doyle, E., Schmidt, C. L., Zhang, F., Hummel, A. W., Bogdanove, A. J., & Voytas, D. F. (2010) · Genetics · doi:10.1534/genetics.110.120717 · Cited by 2,033 · see citing works

The Molecular Genetics of Crop Domestication

Doebley, J., Gaut, B. S., & Smith, B. D. (2006) · Cell · doi:10.1016/j.cell.2006.12.006 · Cited by 1,994 · see citing works

Targeted genome modification of crop plants using a CRISPR-Cas system

Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J., Qiu, J., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2650 · Cited by 1,965 · see citing works

Evolution in Action: Plants Resistant to Herbicides

Powles, S. B., & Yu, Q. P. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042809-112119 · Cited by 1,726 · see citing works

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Xing, H.-L., Dong, L., Wang, Z.-P., Zhang, H.-Y., Han, C.-Y., Liu, B., Wang, X.-C., & Chen, Q.-J. (2014) · BMC Plant Biology · doi:10.1186/s12870-014-0327-y · Cited by 1,684 · see citing works

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,643 · see citing works

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

Xie, K., Minkenberg, B., & Yang, Y. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420294112 · Cited by 1,532 · see citing works

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

Li, J., Norville, J. E., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G. M., & Sheen, J. (2013) · Nature Biotechnology · doi:10.1038/nbt.2654 · Cited by 1,499 · see citing works

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

Woo, J. W., Kim, J., Kwon, S. I., Corvalán, C., Cho, S. W., Kim, H., Kim, S.-G., Kim, S.-T., Choe, S., & Kim, J.-S. (2015) · Nature Biotechnology · doi:10.1038/nbt.3389 · Cited by 1,219 · see citing works

Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

Rodríguez-Leal, D., Lemmon, Z. H., Man, J., Bartlett, M. E., & Lippman, Z. B. (2017) · Cell · doi:10.1016/j.cell.2017.08.030 · Cited by 1,165 · see citing works

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

Nekrasov, V., Staskawicz, B. J., Weigel, D., Jones, J. D. G., & Kamoun, S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2655 · Cited by 1,123 · see citing works

Engineered CRISPR-Cas9 nuclease with expanded targeting space

Nishimasu, H., Shi, X., Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., et al. (2018) · Science · doi:10.1126/science.aas9129 · Cited by 1,100 · see citing works

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

Richter, M. F., Zhao, K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0453-z · Cited by 1,020 · see citing works

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Liang, Z., Chen, K., Li, T., Zhang, Y., Wang, Y., Zhao, Q., Liu, J., Zhang, H., Liu, C., Ran, Y., et al. (2017) · Nature Communications · doi:10.1038/ncomms14261 · Cited by 964 · see citing works

Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds

Zalatan, J. G., Lee, M. E., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, J. E., Qi, L. S., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.052 · Cited by 938 · see citing works

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Zhang, Y., Liang, Z., Zong, Y., Wang, Y., Liu, J., Chen, K., Qiu, J., & Gao, C. (2016) · Nature Communications · doi:10.1038/ncomms12617 · Cited by 906 · see citing works

Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

Chandrasekaran, J., Brumin, M., Wolf, D., Leibman, D., Klap, C., Pearlsman, M., Sherman, A., Arazi, T., & Gal‐On, A. (2016) · Molecular Plant Pathology · doi:10.1111/mpp.12375 · Cited by 883 · see citing works

Morphogenic Regulators <i>Baby boom</i> and <i>Wuschel</i> Improve Monocot Transformation

Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., et al. (2016) · The Plant Cell · doi:10.1105/tpc.16.00124 · Cited by 881 · see citing works

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J., Wang, D., & Gao, C. (2017) · Nature Biotechnology · doi:10.1038/nbt.3811 · Cited by 875 · see citing works

Prime genome editing in rice and wheat

Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · see citing works

Broad-spectrum resistance to bacterial blight in rice using genome editing

Oliva, R. F., Ji, C., Atienza, G., Huguet‐Tapia, J. C., Pérez‐Quintero, Á. L., Li, T., Eom, J., Li, C., Nguyen, H., Liu, B., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0267-z · Cited by 860 · see citing works

De novo domestication of wild tomato using genome editing

Zsögön, A., Čermák, T., Naves, E. R., Notini, M. M., Edel, K. H., Weinl, S., Freschi, L., Voytas, D. F., Kudla, J., & Peres, L. E. P. (2018) · Nature Biotechnology · doi:10.1038/nbt.4272 · Cited by 830 · see citing works

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3833 · Cited by 812 · see citing works

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017) · Scientific Reports · doi:10.1038/s41598-017-00578-x · Cited by 769 · see citing works

Engineering canker‐resistant plants through CRISPR /Cas9‐targeted editing of the susceptibility gene Cs LOB 1 promoter in citrus

Peng, A., Chen, S., Lei, T., Xu, L., He, Y., Liu, W., Yao, L., & Zou, X. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12733 · Cited by 732 · see citing works

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

Lowder, L. G., Zhang, D., Baltes, N. J., Paul, J. W., Tang, X., Zheng, X., Voytas, D. F., Hsieh, T.-F., Zhang, Y., & Qi, Y. (2015) · Plant Physiology · doi:10.1104/pp.15.00636 · Cited by 707 · see citing works

Domestication of wild tomato is accelerated by genome editing

Li, T., Yang, X., Yu, Y., Si, X., Zhai, X., Zhang, H., Dong, W., Gao, C., & Cao, X. (2018) · Nature Biotechnology · doi:10.1038/nbt.4273 · Cited by 664 · see citing works

A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants

Čermák, T., Curtin, S. J., Gil‐Humanes, J., Čegan, R., Kono, T. J. Y., Konečná, E., Belanto, J. J., Starker, C. G., Mathre, J. W., Greenstein, R. L., et al. (2017) · The Plant Cell · doi:10.1105/tpc.16.00922 · Cited by 648 · see citing works

High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants

Demirer, G. S., Zhang, H., Matos, J. L., Goh, N. S., Cunningham, F. J., Sung, Y., Chang, R., Aditham, A. J., Chio, L., Cho, M., et al. (2019) · Nature Nanotechnology · doi:10.1038/s41565-019-0382-5 · Cited by 640 · see citing works

Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers

Kwak, S., Lew, T. T. S., Sweeney, C. J., Koman, V. B., Wong, M. H., Bohmert-Tatarev, K., Snell, K. D., Seo, J. S., Chua, N., & Strano, M. S. (2019) · Nature Nanotechnology · doi:10.1038/s41565-019-0375-4 · Cited by 604 · see citing works

GW5 acts in the brassinosteroid signalling pathway to regulate grain width and weight in rice

Liu, J., Chen, J., Zheng, X., et al. (2017) · Nature Plants · doi:10.1038/nplants.2017.43 · Cited by 596 · see citing works

Low‐gluten, nontransgenic wheat engineered with CRISPR/Cas9

Sánchez‐León, S., Gil‐Humanes, J., Ozuna, C. V., Giménez, M. J., Sousa, C., Voytas, D. F., & Barro, F. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12837 · Cited by 588 · see citing works

Self‐processing of ribozyme‐flanked RNAs into guide RNAs in vitro and in vivo for CRISPR‐mediated genome editing

Gao, Y., & Zhao, Y. (2013) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12152 · Cited by 564 · see citing works

Plant gene editing through de novo induction of meristems

Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0337-2 · Cited by 541 · see citing works

Simultaneous modification of three homoeologs of <i> Ta <scp>EDR</scp> 1 </i> by genome editing enhances powdery mildew resistance in wheat

Zhang, Y., Bai, Y., Wu, G., Zou, S., Chen, Y., Gao, C., & Tang, D. (2017) · The Plant Journal · doi:10.1111/tpj.13599 · Cited by 525 · see citing works

Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase

Sun, Y., Zhang, X., Wu, C., He, Y., Ma, Y., Hou, H., Guo, X., Du, W., Zhao, Y., & Xia, L. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.01.001 · Cited by 522 · see citing works

Rapid improvement of domestication traits in an orphan crop by genome editing

Lemmon, Z. H., Reem, N. T., Dalrymple, J., Soyk, S., Swartwood, K., Rodríguez-Leal, D., Eck, J. M. V., & Lippman, Z. B. (2018) · Nature Plants · doi:10.1038/s41477-018-0259-x · Cited by 520 · see citing works

Directed evolution of adenine base editors with increased activity and therapeutic application

Gaudelli, N. M., Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, D. A., Edwards, A., Gehrke, J. M., Lee, S., Liquori, A., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0491-6 · Cited by 518 · see citing works

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 499 · see citing works

A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds

Khanday, I., Skinner, D. J., Yang, B., Mercier, R., & Sundaresan, V. (2018) · Nature · doi:10.1038/s41586-018-0785-8 · Cited by 470 · see citing works

Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes

Sun, Y., Jiao, G., Liu, Z., Zhang, X., Li, J., Guo, X., Du, W., Du, J., Francis, F., Zhao, Y., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00298 · Cited by 469 · see citing works

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

Zong, Y., Song, Q., Li, C., Jin, S., Zhang, D., Wang, Y., Qiu, J., & Gao, C. (2018) · Nature Biotechnology · doi:10.1038/nbt.4261 · Cited by 433 · see citing works

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

Li, C., Zhang, R., Meng, X., Chen, S., Zong, Y., Lu, C., Qiu, J., Chen, Y., Li, J., & Gao, C. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0393-7 · Cited by 410 · see citing works

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Papikian, A., Liu, W., Gallego‐Bartolomé, J., & Jacobsen, S. E. (2019) · Nature Communications · doi:10.1038/s41467-019-08736-7 · Cited by 407 · see citing works

De Novo Domestication: An Alternative Route toward New Crops for the Future

Fernie, A. R., & Yan, J. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.016 · Cited by 401 · see citing works

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

Li, X., Wang, Y., Chen, S., Tian, H., Fu, D., Zhu, B., Luo, Y., & Zhu, H. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00559 · Cited by 392 · see citing works

Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs

Ellison, E. E., Nagalakshmi, U., Gamo, M. E., Huang, P., Dinesh‐Kumar, S. P., & Voytas, D. F. (2020) · Nature Plants · doi:10.1038/s41477-020-0670-y · Cited by 377 · see citing works

High‐frequency homologous recombination in plants mediated by zinc‐finger nucleases

Wright, D. A., Townsend, J. A., Winfrey, R. J., Irwin, P. A., Rajagopal, J., Lonosky, P. M., Hall, B. D., Jondle, M. D., & Voytas, D. F. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02551.x · Cited by 370 · see citing works

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

Ali, Z., Abulfaraj, A. A., Li, L., Ghosh, N., Piatek, M. J., Mahjoub, A., Aouida, M., Piatek, A., Baltes, N. J., Voytas, D. F., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.02.011 · Cited by 368 · see citing works

Genome editing of upstream open reading frames enables translational control in plants

Zhang, H., Si, X., Ji, X., Fan, R., Liu, J., Chen, K., Wang, D., & Gao, C. (2018) · Nature Biotechnology · doi:10.1038/nbt.4202 · Cited by 364 · see citing works

Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9

Ma, X., Zhang, X., Liu, H., & Li, Z. (2020) · Nature Plants · doi:10.1038/s41477-020-0704-5 · Cited by 359 · see citing works

Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes

Wang, C., Liu, Q., Shen, Y. X., Hua, Y., Wang, J., Lin, J., Wu, M., Sun, T., Cheng, Z., Mercier, R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-018-0003-0 · Cited by 357 · see citing works

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Dong, O. X., Yu, S., Jain, R., Zhang, N., Duong, P. Q., Butler, C., Li, Y., Lipzen, A., Martin, J., Barry, K., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-14981-y · Cited by 356 · see citing works

A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize

Liu, C., Li, X., Meng, D., Zhong, Y., Chen, C., Dong, X., Xu, X., Chen, B., Li, W., Li, L., et al. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.01.011 · Cited by 353 · see citing works

One-step genome editing of elite crop germplasm during haploid induction

Kelliher, T., Starr, D., Su, X., Tang, G., Chen, Z., Carter, J., Wittich, P. E., Dong, S., Green, J., Burch, E., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0038-x · Cited by 349 · see citing works

Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing

Zhang, R., Liu, J., Chai, Z., Chen, S., Bai, Y., Zong, Y., Chen, K., Li, J., Jiang, L., & Gao, C. (2019) · Nature Plants · doi:10.1038/s41477-019-0405-0 · Cited by 340 · see citing works

Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice

Xu, Z., Xu, X., Gong, Q., Li, Z., Li, Y., Wang, S., Yang, Y., Ma, W., Liu, L., Zhu, B., et al. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.08.006 · Cited by 340 · see citing works

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

Wang, M., Mao, Y., Lu, Y., Tao, X., & Zhu, J. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.03.001 · Cited by 331 · see citing works

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Lian, J., HamediRad, M., Hu, S., & Zhao, H. (2017) · Nature Communications · doi:10.1038/s41467-017-01695-x · Cited by 319 · see citing works

Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

Lowe, K. S., Rota, M. L., Hoerster, G., Hastings, C., Wang, N., Chamberlin, M. A., Wu, E., Jones, T. J., & Gordon-Kamm, W. J. (2018) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-018-9905-2 · Cited by 319 · see citing works

Rational Improvement of Rice Yield and Cold Tolerance by Editing the Three Genes OsPIN5b, GS3, and OsMYB30 With the CRISPR–Cas9 System

Zeng, Y., Wen, J., Zhao, W., Wang, Q., & Huang, W. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01663 · Cited by 319 · see citing works

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

Lu, Y., Ye, X., Guo, R., Huang, J., Wang, W., Tang, J., Tan, L., Zhu, J., Chu, C., & Qian, Y. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.06.007 · Cited by 306 · see citing works

Precision genome engineering through adenine base editing in plants

Kang, B., Yun, J., Kim, S., Shin, Y., Ryu, J., Choi, M., Woo, J. W., & Kim, J. (2018) · Nature Plants · doi:10.1038/s41477-018-0178-x · Cited by 306 · see citing works

Analysis of the functions of Ta GW 2 homoeologs in wheat grain weight and protein content traits

Zhang, Y., Li, D., Zhang, D., Zhao, X., Cao, X., Dong, L., Liu, J., Chen, K., Zhang, H., Gao, C., et al. (2018) · The Plant Journal · doi:10.1111/tpj.13903 · Cited by 305 · see citing works

DNA nanostructures coordinate gene silencing in mature plants

Zhang, H., Demirer, G. S., Zhang, H., Ye, T., Goh, N. S., Aditham, A. J., Cunningham, F. J., Fan, C., & Landry, M. P. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1818290116 · Cited by 292 · see citing works

Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease

Puchta, H., Dujon, B., & Höhn, B. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.22.5034 · Cited by 291 · see citing works

Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain

Gallego‐Bartolomé, J., Gardiner, J., Liu, W., Papikian, A., Ghoshal, B., Kuo, H. Y., Zhao, J. M.-C., Segal, D. J., & Jacobsen, S. E. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1716945115 · Cited by 290 · see citing works

Multiplexed CRISPR/Cas9‐mediated metabolic engineering of γ‐aminobutyric acid levels in Solanum lycopersicum

Li, R., Li, R., Li, X., et al. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12781 · Cited by 288 · see citing works

Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

Campa, C. C., Weisbach, N. R., Santinha, A. J., Incarnato, D., & Platt, R. J. (2019) · Nature Methods · doi:10.1038/s41592-019-0508-6 · Cited by 284 · see citing works

Mutation of ZmDMP enhances haploid induction in maize

Zhong, Y., Liu, C., Qi, X., et al. (2019) · Nature Plants · doi:10.1038/s41477-019-0443-7 · Cited by 283 · see citing works

Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

Morgan, S. L., Mariano, N. C., Bermudez, A., Arruda, N. L., Wu, F., Luo, Y., Shankar, G., Jia, L., Chen, H., Hu, J.-F., et al. (2017) · Nature Communications · doi:10.1038/ncomms15993 · Cited by 279 · see citing works

With a free pass, CRISPR-edited plants reach market in record time

Waltz, E. (2018) · Nature Biotechnology · doi:10.1038/nbt0118-6b · Cited by 279 · see citing works

A potent Cas9-derived gene activator for plant and mammalian cells

Li, Z., Zhang, D., Xiong, X., Yan, B., Xie, W., Sheen, J., & Li, J. (2017) · Nature Plants · doi:10.1038/s41477-017-0046-0 · Cited by 278 · see citing works

OsMATL mutation induces haploid seed formation in indica rice

Yao, L., Zhang, Y. P., Liu, C., Liu, Y., Wang, Y., Liang, D., Liu, J., Sahoo, G., & Kelliher, T. (2018) · Nature Plants · doi:10.1038/s41477-018-0193-y · Cited by 276 · see citing works

Blocking amino acid transporter Os AAP 3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Lü, K., Wu, B., Wang, J., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12907 · Cited by 275 · see citing works

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

Meng, X., Yu, H. J., Zhang, Y., Zhuang, F., Song, X., Gao, S., Gao, C., & Li, J. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.06.006 · Cited by 273 · see citing works

Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms

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Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding

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Expanding the base editing scope in rice by using Cas9 variants

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ENO regulates tomato fruit size through the floral meristem development network

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Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

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Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

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