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Human mesenchymal stem cells maintain their phenotype, multipotentiality, and genetic stability when cultured using a defined xeno-free human plasma fraction

Blázquez-Prunera, Arantxa, Díez, José María, Gajardo, Rodrigo, Grancha, Salvador·2017·Stem Cell Research & Therapy

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  • Acceptable intervals for periodic testing to confirm the genetic and phenotypic stability of the cell system can be highly case-dependent.

    “It is recommended to develop acceptable intervals for periodic testing to confirm the genetic and phenotypic stability of the cell system; these, however, can be highly case-dependent (Blazquez-Prunera et al., 2017; Daily et al., 2017; Meza-Zepeda et al., 2008).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Blazquez-Prunera et al., 2017

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Research works printed but not linked: 47

  1. [1]Singer NG Caplan AI Mesenchymal stem cells: mechanisms of inflammation Annu Rev Pathol. 2011 6 457 78 10.1146/annurev-pathol-011110-130230 21073342 · DOI
  2. [2]Horwitz EM Le Blanc K Dominici M Mueller I Slaper-Cortenbach I Marini FC Deans RJ Krause DS Keating A International Society for Cellular Therapy Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement Cytotherapy. 2005 7 393 5 10.1080/14653240500319234 16236628 · DOI
  3. [3]Singec I Janrial R Crain A Nikkhah G Snyder EY The leading edge of stem cell therapeutics Annu Rev Med. 2007 58 313 28 10.1146/annurev.med.58.070605.115252 17100553 · DOI
  4. [5]Aldahmash A Haack-Sørensen M Al-Nbaheen M Harkness L Abdallah BM Kassem M Human serum is as efficient as fetal bovine serum in supporting proliferation and differentiation of human multipotent stromal (mesenchymal) stem cells in vitro and in vivo Stem Cell Rev. 2011 7 860 8 10.1007/s12015-011-9274-2 21603946 · DOI
  5. [6]Bieback K Hecker A Kocaömer A Lannert H Schallmoser K Strunk D Klüter H Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow Stem Cells. 2009 27 2331 41 10.1002/stem.139 19544413 · DOI
  6. [7]Spees JL Gregory CA Singh H Tucker HA Peister A Lynch PJ Hsu SC Smith J Prockop DJ Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy Mol Ther. 2004 9 747 56 10.1016/j.ymthe.2004.02.012 15120336 · DOI
  7. [8]Jayme DW Smith SR Media formulation options and manufacturing process controls to safeguard against introduction of animal origin contaminants in animal cell culture Cytotechnology. 2000 33 27 36 10.1023/A:1008133717035 19002808 PMC3466725 · DOI
  8. [9]Jung S Panchalingam KM Rosenberg L Behie LA Ex vivo expansion of human mesenchymal stem cells in defined serum-free media Stem Cells Int. 2012 2012 123030 10.1155/2012/123030 22645619 PMC3356989 · DOI
  9. [11]Lennon DP Haynesworth SE Young RG Dennis JE Caplan AI A chemically defined medium supports in vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem cells Exp Cell Res. 1995 1 211 22 10.1006/excr.1995.1221 7628536 · DOI
  10. [12]Parker AM Shang H Khurgel M Katz A Low serum and serum-free culture of multipotential human adipose stem cells Cytotherapy. 2007 9 637 46 10.1080/14653240701508452 17917877 · DOI
  11. [13]Lindroos B Boucher S Chase L Kuokkanen H Huhtala H Haataja R Vemuri M Suuronen R Miettinen S Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro Cytotherapy. 2009 11 958 72 10.3109/14653240903233081 19903107 · DOI
  12. [14]Miwa H Hashimoto Y Tensho K Wakitani S Takagi M Xeno-free proliferation of human bone marrow mesenchymal stem cells Cytotechnology. 2012 64 301 8 10.1007/s10616-011-9400-7 22002865 PMC3386392 · DOI
  13. [15]Schallmoser K Rohde E Reinisch A Bartmann C Thaler D Drexler C Obenauf AC Lanzer G Linkesch W Strunk D Rapid largescale expansion of functional mesenchymal stem cells from unmanipulated bone marrow without animal serum Tissue Eng Part C Methods. 2008 14 185 96 10.1089/ten.tec.2008.0060 18620484 · DOI
  14. [16]Liu CH Wu ML Hwang SM Optimization of serum free medium for cord blood mesenchymal stem cells Biochem Eng J. 2007 33 1 9 10.1016/j.bej.2006.08.005 · DOI
  15. [17]Hartmann I Hollweck T Haffner S Krebs M Meiser B Reichart B Eissner G Umbilical cord tissue-derived mesenchymal stem cells grow best under GMP-compliant culture conditions and maintain their phenotypic and functional properties J Immunol Methods. 2010 363 80 9 10.1016/j.jim.2010.10.008 21035451 · DOI
  16. [18]Oikonomopoulos A van Deen WK Manansala AR Lacey PN Tomakili TA Ziman A Hommes DW Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media Sci Rep. 2015 5 16570 10.1038/srep16570 26564250 PMC4643287 · DOI
  17. [19]Chase LG Yang S Zachar V Yang Z Lakshmipathy U Bradford J Boucher SE Vemuri MC Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells Stem Cells Transl Med. 2012 1 750 8 10.5966/sctm.2012-0072 23197667 PMC3659659 · DOI
  18. [20]Corotchi MC Popa MA Remes A Sima LE Gussi I Lupu PM Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton’s jelly-derived mesenchymal stem cells Stem Cell Res Ther. 2013 4 81 10.1186/scrt232 23845279 PMC3854854 · DOI
  19. [21]Patrikoski M Juntunen M Boucher S Campbell A Vemuri MC Mannerström B Miettinen S Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells Stem Cell Res Ther. 2013 4 27 10.1186/scrt175 23497764 PMC3707027 · DOI
  20. [22]Simoes IN Boura JS Dos Santos F Andrade PZ Cardoso CM Gimble JM da Silva CL Cabral JM Human mesenchymal stem cells from the umbilical cord matrix: successful isolation and ex-vivo expansion using serum-/xeno-free culture media Biotechnol J. 2013 8 448 58 10.1002/biot.201200340 23420807 · DOI
  21. [23]Gstraunthaler G Alternatives to the use of fetal bovine serum: serum-free cell culture ALTEX. 2003 20 275 81 14671707
  22. [24]Li CY Wu XY Tong JB Yang XX Zhao JL Zheng QF Zhao GB Ma ZJ Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy Stem Cell Res Ther. 2015 13 6 55 PMC4453294 25884704
  23. [25]Doucet C Ernou I Zhang Y Llense JR Begot L Holy X Lataillade JJ Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications J Cell Physiol. 2005 205 228 36 10.1002/jcp.20391 15887229 · DOI
  24. [26]Schallmoser K Bartmann C Rohde E Reinisch A Kashofer K Stadelmeyer E Drexler C Lanzer G Linkesch W Strunk D Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells Transfusion. 2007 47 1436 46 10.1111/j.1537-2995.2007.01220.x 17655588 · DOI
  25. [27]Cohn EJ Strong LE Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids J Am Chem Soc. 1946 68 459 75 10.1021/ja01207a034 21015743 · DOI
  26. [28]Lange C Cakiroglu F Spiess AN Cappallo-Obermann H Dierlamm J Zander AR Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine J Cell Physiol. 2007 213 18 26 10.1002/jcp.21081 17458897 · DOI
  27. [29]Díez JM Bauman E Gajardo R Jorquera JI Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools Stem Cell Res Ther. 2015 6 28 10.1186/s13287-015-0016-2 25889980 PMC4396121 · DOI
  28. [30]Blázquez-Prunera A, Almeida CR, Barbosa MA. Human bone marrow mesenchymal stem/stromal cells preserve their immunomodulatory and chemotactic properties when expanded in a human plasma-derived xeno-free medium. Stem Cells International. 2017 (in press). PMC5446864 28588620
  29. [31]Schallmoser K Strunk D Preparation of pooled human platelet lysate (pHPL) as an efficient supplement for animal serum-free human stem cell cultures J Vis Exp. 2009 32 1523 PMC3164065 19881465
  30. [32]Tunaitis V Borutinskaité V Navakauskiené R Treigytė G Ungurytė A Aldonytė R Magnusson KE Pivoriūnas A Effects of different sera on adipose tissue-derived mesenchymal stromal cells J Tissue Eng Regen Med. 2011 5 733 46 10.1002/term.374 21953871 · DOI
  31. [33]Dahl JA Duggal S Coulston N Millar D Melki J Shahdadfar A Brinchmann JE Collas P Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fatal bovine serum Int J Dev Biol. 2008 52 1033 42 10.1387/ijdb.082663jd 18956336 · DOI
  32. [34]Mizuno N Shiba H Ozeki Y Mouri Y Niitani M Inui T Hayashi H Suzuki K Tanaka S Kawaguchi H Kurihara H Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures Cell Biol Int. 2006 30 521 4 10.1016/j.cellbi.2006.01.010 16616867 · DOI
  33. [35]Stute N Holtz K Bubenheim M Lange C Blake F Zander AR Autologous serum for isolation and expansion of human mesenchymal stemcells for clinical use Exp Hematol. 2004 32 1212 25 10.1016/j.exphem.2004.09.003 15588946 · DOI
  34. [36]Shahdadfar A Frønsdal K Haug T Reinholt FP Brinchmann JE In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability Stem Cells. 2005 23 1357 66 10.1634/stemcells.2005-0094 16081661 · DOI
  35. [37]Tateishi K Ando W Higuchi C Hart DA Hashimoto J Nakata K Yoshikawa H Nakamura N Comparison of human serum with fetal bovine serum for expansion and differentiation of human synovial MSC: potential feasibility for clinical applications Cell Transplant. 2008 17 549 57 10.3727/096368908785096024 18714674 · DOI
  36. [38]Kuznetsov SA Mankani MH Robey PG Effect of serum on human bone marrow stromal cells: ex vivo expansion and in vivo bone formation Transplantation. 2000 70 1780 7 10.1097/00007890-200012270-00018 11152111 · DOI
  37. [39]Poloni A Maurizi G Rosini V Mondini E Mancini S Discepoli G Biasio S Battaglini G Felicetti S Berardinelli E Serrani F Leoni P Selection of CD271+ cells and human AB serum allows a large expansion of mesenchymal stromal cells from human bone marrow Cytotherapy. 2009 11 153 62 10.1080/14653240802582125 19301169 · DOI
  38. [40]Le Blanc K Samuelsson H Lönnies L Sundin M Ringdén O Generation of immunosuppressive mesenchymal stem cells in allogeneic human serum Transplantation. 2007 84 1055 9 10.1097/01.tp.0000285088.44901.ea 17989613 · DOI
  39. [41]Kocaoemer A Kern S Kluter H Bieback K Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue Stem Cells. 2007 25 1270 8 10.1634/stemcells.2006-0627 17255520 · DOI
  40. [42]Dominici M Le Blanc K Mueller I Slaper-Cortenbach I Marini F Krause D Deans R Keating A Prockop DJ Horwitz E Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Cytotherapy. 2006 8 315 7 10.1080/14653240600855905 16923606 · DOI · Paper page
  41. [43]Zeddou M Relic B Malaise MG Umbilical cord fibroblasts: Could they be considered as mesenchymal stem cells? World J Stem Cells. 2014 6 367 70 10.4252/wjsc.v6.i3.367 25126385 PMC4131277 · DOI
  42. [44]Nagamura-Inoue T He H Umbilical cord-derived mesenchymal stem cells: Their advantages and potential clinical utility World J Stem Cells. 2014 6 195 202 10.4252/wjsc.v6.i2.195 24772246 PMC3999777 · DOI
  43. [45]Lohmann M Walenda G Hemeda H Joussen S Drescher W Jockenhoevel S Hutschenreuter G Zenke M Wagner W Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells Plos One. 2012 7 10.1371/journal.pone.0037839 22662236 PMC3360602 · DOI
  44. [46]Lange C Brunswig-Spickenheier B Eissing L Scheja L Platelet lysate suppresses the expression of lipocalin-type prostaglandin D2 synthase that positively controls adipogenic differentiation of human mesenchymal stromal cells Exp Cell Res. 2012 318 2284 96 10.1016/j.yexcr.2012.06.004 22698646 · DOI
  45. [47]Gruber R Karreth F Kandler B Fuerst G Rot A Fischer MB Watzek G Platelet-released supernatants increase migration and proliferation, and decrease osteogenic differentiation of bone marrow-derived mesenchymal progenitor cells under in vitro conditions Platelets. 2004 15 29 35 10.1080/09537100310001643999 14985174 · DOI
  46. [48]Abdelrazik H Spaggiari GM Chiossone L Moretta L Mesenchymal stem cells expanded in human platelet lysate display a decreased inhibitory capacity on T- and NK-cell proliferation and function Eur J Immunol. 2011 41 3281 90 10.1002/eji.201141542 21874650 · DOI
  47. [49]Copland IB Garcia MA Waller EK Roback JD Galipeau J The effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy Biomaterials. 2013 34 7840 50 10.1016/j.biomaterials.2013.06.050 23891515 · DOI

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Authors
Blázquez-Prunera, A., Díez, J. M., Gajardo, R., & Grancha, S.
Venue
Stem Cell Research & Therapy
Published
2017-04-27
Volume
8
Issue
1
ISSN
1757-6512
Cited by
55
References
0
Language
en

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