The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Mesenchymal stem cells and extracellular matrix scaffold promote muscle regeneration by synergistically regulating macrophage polarization toward the M2 phenotype
Open accessAbstract
BACKGROUND: Skeletal muscle plays an important role in the body's physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood. METHODS: The characteristics of human umbilical cord MSCs, including multiplication capacity and multidifferentiation ability, were determined. We constructed a compound containing MSCs and decellularized ECM scaffold which was used for tissue regeneration in a VML model. RESULTS: We found that MSCs and decellularized ECM scaffold generated synergistic effects on promoting skeletal muscle tissue regeneration. Interestingly, both MSCs and decellularized ECM scaffold could promote macrophage polarization toward the M2 phenotype and suppress macrophage polarization toward the M1 phenotype, which is widely regarded as an important promoting factor in tissue regeneration. More importantly, MSCs and decellularized ECM scaffold generate synergistic promoting effects on macrophage polarization toward the M2 phenotype, not just an additive effect. CONCLUSIONS: Our findings uncover a previously unknown mechanism that MSCs and decellularized ECM scaffold promote tissue regeneration via collaboratively regulating macrophage polarization.
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“Several physical factors of ECMs have been evidenced to influence macrophages polarization and determine the outcomes of wound healing and tissue remodeling, including stiffness, topography and so on [[27], [28], [29]].”
From Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-κB signaling pathway (2020) · cites this paper as [29]
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An overview of tissue and whole organ decellularization processes
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Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart
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Multipotent mesenchymal stromal cells and the innate immune system
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Macrophages in skin injury and repair
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HucMSC-Exosome Mediated-Wnt4 Signaling Is Required for Cutaneous Wound Healing
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Self-citationZhang, J., Guan, J., Niu, X., Hu, G., Guo, S., Li, Q., Xie, Z., Zhang, C., & Wang, Y. (2015) · Journal of Translational Medicine · doi:10.1186/s12967-015-0417-0 · Cited by 804
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Chen, M., Zhang, Y., Zhou, P., Liu, X., Zhao, H., Zhou, X., Gu, Q., Li, B., Zhu, X., & Shi, Q. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.05.004 · Cited by 229
Macrophage polarization in periodontal ligament stem cells enhanced periodontal regeneration
Liu, J., Chen, B., Bao, J., Zhang, Y., Lei, L., & Yan, F. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1409-4 · Cited by 198
Macrophages Are Key Regulators of Stem Cells during Skeletal Muscle Regeneration and Diseases
Dort, J., Fabre, P., Molina, T., & Dumont, N. A. (2019) · Stem Cells International · doi:10.1155/2019/4761427 · Cited by 211
Vascularized and Innervated Skeletal Muscle Tissue Engineering
Gilbert‐Honick, J., & Grayson, W. L. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201900626 · Cited by 197
Zhang, B., Su, Y., Zhou, J., Zheng, Y., & Zhu, D. (2021) · Advanced Science · doi:10.1002/advs.202100446 · Cited by 181
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Metadata
- Authors
- Qiu, X., Liu, S., Zhang, H., Zhu, B., Su, Y., Zheng, C., Tian, R., Wang, M., Kuang, H., Zhao, X., & Jin, Y.
- Affiliations
- Air Force Medical University (CN)
- Chinese People's Liberation Army (CN)
- Corresponding author
- Zhao, Xinyi
- Jin, Yan
- Venue
- Stem Cell Research & Therapy
- Published
- 2018-04-03
- Volume
- 9
- Issue
- 1
- Pages
- 88-88
- ISSN
- 1757-6512 (ISSN-L)
- PMC
- PMC5883419
- OpenAlex
- W2800380986
- Cited by
- 111
- References
- 64
- Citations by year
- 2026: 5 · 2025: 12 · 2024: 13 · 2023: 7 · 2022: 17 · 2021: 17 · 2020: 21 · 2019: 13 · 2018: 6
- FWCI
- 14.81
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Research & Therapy (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31670995, 81470679)
- National Key Research and Development Program of China (2016YFC1101400, 2016TFC1102903)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- mesenchymal stem cells, skeletal muscle regeneration, volumetric muscle loss, macrophage polarization, M2 macrophages, M1 macrophages, human umbilical cord mesenchymal stem cells, synergistic effect, tissue engineering, traumatic muscle injury
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Extracellular Matrix, Humans, Macrophages, Regeneration, Rats, Sprague-Dawley, Muscle, Skeletal, Mesenchymal Stem Cell Transplantation, Rats, Mesenchymal Stem Cells
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