Cited by 275Mesenchymal stem cell researchAdipose Tissue and MetabolismPeriodontal Regeneration and Treatments

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Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue

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Aronowitz, Joel A., Lockhart, Ryan A., Hakakian, Cloe S.·2015·SpringerPlus

DOI 10.1186/s40064-015-1509-2PMC PMC4656256

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Abstract

Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly expanding in medicine. Most commonly, ASCs are isolated at the point of care from lipoaspirate tissue as the stromal vascular fraction (SVF). The cells are immediately administered to the patient as an injection or used to enrich fat grafts. Isolation of ASCs from adipose tissue is a relatively simple process performed routinely in cell biology laboratories, but isolation at the point of care for immediate clinical administration requires special methodology to prevent contamination, ensure integrity of clinical research and comply with regulatory requirements. A lack of practical laboratory experience, regulatory uncertainty and a relative paucity of objective published data can make selection of the optimum separation method for specific indications a difficult task for the clinician and can discourage clinical adoption. In this paper, we discuss the processes which can be used to separate SVF cells from fat tissue. We compare the various mechanical and enzymatic methods. We discuss the practical considerations involved in selecting an appropriate method from a clinical perspective. Studies consistently show that breakdown of the extracellular matrix achieved with proteolytic enzymes affords significantly greater efficiency to the separation process. SVF isolated through mechanical methods is equally safe, less costly and less time consuming but the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells. Mechanical methods can provide a low cost, rapid and simple alternative to enzymatic isolation methods, and are attractive when smaller quantities of ASCs are sufficient.

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Authors: Aronowitz, J. A., Lockhart, R. A., & Hakakian, C. S.
Affiliations: University of Southern California (US)
Corresponding author: Aronowitz, Joel A.
Venue: SpringerPlus
Publisher: Springer Science and Business Media LLC
Published: 2015-11-23
Volume: 4
Issue: 1
Pages: 713-713
ISSN: 2193-1801 (ISSN-L)
DOI: 10.1186/s40064-015-1509-2
PMC: PMC4656256
OpenAlex: W2173002160
Cited by: 275
References: 55
Citations by year: 2026: 26 · 2025: 24 · 2024: 40 · 2023: 27 · 2022: 43 · 2021: 30 · 2020: 22 · 2019: 27 · 2018: 19 · 2017: 12 · 2016: 4
FWCI: 6.53
Type: journal article
Language: en
Open access: diamond
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0 (unspecified)
Locations: SpringerPlus (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
Keywords: adipose-derived stem cells, stromal vascular fraction, enzymatic cell isolation, proteolytic enzymes, lipoaspirate, progenitor cells, fat graft

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Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue

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