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Cited by 4Spectroscopy Techniques in Biomedical and Chemical ResearchMultiple Sclerosis Research StudiesSpectroscopy and Chemometric Analyses

Infrared spectral profiling of demyelinating activity in multiple sclerosis brain tissue

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Gakh, Oleksandr, Wilkins, Jordan M., Guo, Yong, Popescu, Bogdan F., Weigand, Stephen D., Kalinowska-Lyszczarz, Alicja, Lucchinetti, Claudia F.·2024·Acta Neuropathologica Communications
DOI 10.1186/s40478-024-01854-4PMC PMC11385516
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Abstract

Multiple sclerosis (MS) is a leading cause of non-traumatic disability in young adults. The highly dynamic nature of MS lesions has made them difficult to study using traditional histopathology due to the specificity of current stains. This requires numerous stains to track and study demyelinating activity in MS. Thus, we utilized Fourier transform infrared (FTIR) spectroscopy to generate holistic biomolecular profiles of demyelinating activities in MS brain tissue. Multivariate analysis can differentiate MS tissue from controls. Analysis of the absorbance spectra shows profound reductions of lipids, proteins, and phosphate in white matter lesions. Changes in unsaturated lipids and lipid chain length indicate oxidative damage in MS brain tissue. Altered lipid and protein structures suggest changes in MS membrane structure and organization. Unique carbohydrate signatures are seen in MS tissue compared to controls, indicating altered metabolic activities. Cortical lesions had increased olefinic lipid content and abnormal membrane structure in normal appearing MS cortex compared to controls. Our results suggest that FTIR spectroscopy can further our understanding of lesion evolution and disease mechanisms in MS paving the way towards improved diagnosis, prognosis, and development of novel therapeutics.

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  • In demyelinating and dysmyelinating models, phosphoinositide distributions within white matter tracts are altered.

    “In demyelinating and dysmyelinating models, phosphoinositide distributions within white matter tracts are altered [85–87]”

    From PLCγ2 deficiency compromises systemic immune tolerance and erodes myelin homeostasis while enhancing oxidative metabolism in the mouse brain (2026) · cites this paper as [87]

    The Results section reports reduced phosphate signals in demyelinated MS white matter, and the Discussion interprets these as likely reflecting phospholipid changes. The paper does not identify phosphoinositide distributions specifically or establish the claim for demyelinating and dysmyelinating models; it examines MS brain tissue. (LLM, conf=0.86)

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References 62 · Cited by 4

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Authors
Gakh, O., Wilkins, J. M., Guo, Y., Popescu, B. F., Weigand, S. D., Kalinowska-Lyszczarz, A., & Lucchinetti, C. F.
Affiliations
  • Mayo Clinic (US)
  • University of Saskatchewan (CA)
  • The University of Texas at Austin (US)
Corresponding author
Lucchinetti, Claudia F.
ORCID
Venue
Acta Neuropathologica Communications
Publisher
Springer Science and Business Media LLC
Published
2024-09-10
Volume
12
Issue
1
Pages
146-146
ISSN
2051-5960 (ISSN-L)
DOI
10.1186/s40478-024-01854-4
PMC
PMC11385516
OpenAlex
W4402405347
Cited by
4
References
62
Citations by year
2026: 2 · 2025: 2
FWCI
0.77
Type
journal article
Language
en
Open access
Licence
Locations
  • Acta Neuropathologica Communications (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
Keywords
multiple sclerosis, demyelination, brain tissue, white matter lesions, cortical lesions, lipid oxidation, membrane structure, unsaturated lipids, lipid chain length, carbohydrate metabolism, protein structure, phosphate, lesion evolution
MeSH terms
Adult, Brain, Female, Humans, Male, Middle Aged, Multiple Sclerosis, Spectroscopy, Fourier Transform Infrared, White Matter

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