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Cited by 186Plant Pathogens and Fungal DiseasesFungal Plant Pathogen ControlYeasts and Rust Fungi Studies

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Identification, prevalence and pathogenicity of Colletotrichum species causing anthracnose of Capsicum annuum in Asia

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de Silva, Dilani D., Groenewald, Johannes Z., Crous, Pedro W., Ades, Peter K., Nasruddin, Andi, Mongkolporn, Orarat, Taylor, Paul W. J.·2019·IMA Fungus
DOI 10.1186/s43008-019-0001-yPMC PMC7184891
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Abstract

Anthracnose of chili ( Capsicum spp.) causes major production losses throughout Asia where chili plants are grown. A total of 260 Colletotrichum isolates, associated with necrotic lesions of chili leaves and fruit were collected from chili producing areas of Indonesia, Malaysia, Sri Lanka, Thailand and Taiwan. Colletotrichum truncatum was the most commonly isolated species from infected chili fruit and was readily identified by its falcate spores and abundant setae in the necrotic lesions. The other isolates consisted of straight conidia (cylindrical and fusiform) which were difficult to differentiate to species based on morphological characters. Taxonomic analysis of these straight conidia isolates based on multi-gene phylogenetic analyses (ITS , gapdh, chs-1, act, tub2, his3, ApMat, gs ) revealed a further seven known Colletotrichum species, C. endophyticum, C. fructicola, C. karsti, C. plurivorum, C. scovillei , C. siamense and C. tropicale . In addition, three novel species are also described as C. javanense, C. makassarense and C. tainanense , associated with anthracnose of chili fruit in West Java (Indonesia); Makassar, South Sulawesi (Indonesia); and Tainan (Taiwan), respectively. Colletotrichum siamense is reported for the first time causing anthracnose of Capsicum annuum in Indonesia and Sri Lanka. This is also the first report of C. fructicola causing anthracnose of chili in Taiwan and Thailand and C. plurivorum in Malaysia and Thailand. Of the species with straight conidia, C. scovillei (acutatum complex), was the most prevalent throughout the surveyed countries, except for Sri Lanka from where this species was not isolated. Colletotrichum siamense (gloeosporioides complex) was also common in Indonesia, Sri Lanka and Thailand. Pathogenicity tests on chili fruit showed that C. javanense and C. scovillei were highly aggressive, especially when inoculated on non-wounded fruit, compared to all other species. The existence of new, highly aggressive exotic species, such as C. javanense , poses a biosecurity risk to production in countries which do not have adequate quarantine regulations to restrict the entry of exotic pathogens.

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Metadata

Authors
de Silva, D. D., Groenewald, J. Z., Crous, P. W., Ades, P. K., Nasruddin, A., Mongkolporn, O., & Taylor, P. W. J.
Affiliations
  • The University of Melbourne (AU)
  • Westerdijk Fungal Biodiversity Institute (NL)
  • Hasanuddin University (ID)
  • Kasetsart University (TH)
Corresponding author
de Silva, Dilani D.
ORCID
Venue
IMA Fungus
Publisher
Pensoft Publishers
Published
2019-06-28
Volume
10
Issue
1
Pages
8-8
ISSN
  • 2210-6340 (ISSN-L)
  • 2210-6359
DOI
10.1186/s43008-019-0001-y
PMC
PMC7184891
OpenAlex
W2954513703
Cited by
186
References
4
Citations by year
2026: 12 · 2025: 34 · 2024: 23 · 2023: 42 · 2022: 24 · 2021: 32 · 2020: 17 · 2019: 2
FWCI
9.93
Type
journal article
Language
en
Open access
Licence
Locations
  • IMA Fungus (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • KNAW research portal (Royal Academy of Art and Sciences (KNAW)) (publishedVersion, other-oa)
  • KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion, other-oa)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Greater South Information System
Funders
  • Chiang Mai University
  • University of Ruhuna
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
  • Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
Keywords
Colletotrichum, anthracnose, Capsicum annuum, chili pepper, Colletotrichum truncatum, Colletotrichum scovillei, Colletotrichum siamense, new fungal species, multilocus phylogeny, pathogenicity, biosecurity

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