Cited by 5Genomics and Chromatin DynamicsAcute Myeloid Leukemia ResearchRetinoids in leukemia and cellular processes

Characterization of promoter elements directing Mona/Gads molecular adapter expression in T and myelomonocytic cells: involvement of the AML-1 transcription factor

Guyot, B, Mouchiroud, G·2003·Journal of Leukocyte Biology

Abstract

Monocytic adaptor (Mona, also called Gads) is a molecular adaptor implicated in T cell activation and macrophage differentiation. The objective of this study was to identify elements regulating specific expression of Mona/Gads in human T cell and myelomonocytic cell lines. We first confirmed that the −2000 to +150 genomic region relative to the Mona gene transcription start site is sufficient to direct specific reporter gene expression in T cell lines, Jurkat, and MOLT-4 and in the immature myeloid cell lines, KG1a and RC2A. Deletion analysis and electrophoresis mobility shift assay identified several cis regulatory elements: overlapping initiator sequences, one interferon response factor-2 (IRF-2)-binding site at position −154, one GC box recognized by Sp1 and Sp3 at position −52, and two acute myeloid leukemia (AML)-1 binding sites at positions −70 and −13. Site-directed mutagenesis experiments indicated a key role of AML-1 for driving Mona expression in T cells and myeloid cells, and involvement of Sp1/Sp3 and IRF-2 transcription factors to modulate Mona expression in a cell-specific manner.

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Crossref · 10.1189/jlb.0502244

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Authors
Guyot, B., & Mouchiroud, G.
Affiliations
Lyon 1 Université (FR)
Corresponding author
Mouchiroud, G
Venue
Journal of Leukocyte Biology
Published
2003-02-01
Volume
73
Issue
2
Pages
263-272
ISSN
  • 0741-5400 (ISSN-L)
  • 1938-3673
OpenAlex
W2170619973
Cited by
5
References
48
Citations by year
2023: 1 · 2021: 1
FWCI
0.11
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Leukocyte Biology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • Centre National de la Recherche Scientifique
  • Ligue Nationale contre le Cancer
  • Fondation Mérieux and Association pour la Recherche contre le Cancer
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
Keywords
promoter elements, IRF2, Sp1 binding site, T cell activation, myelomonocytic cells, transcriptional regulation, macrophage differentiation, Jurkat cells, MOLT-4 cells, KG1a cells
MeSH terms
Base Sequence, Carrier Proteins, Cells, Cultured, DNA-Binding Proteins, Humans, Molecular Sequence Data, Promoter Regions, Genetic, Proto-Oncogene Proteins, Repressor Proteins, T-Lymphocytes, Transcription Factors, Transcription, Genetic, Sp1 Transcription Factor, 5' Untranslated Regions, 5' Flanking Region, Adaptor Proteins, Signal Transducing, Core Binding Factor Alpha 2 Subunit, Interferon Regulatory Factor-2, Sp3 Transcription Factor

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