The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide
Abstract
A variety of inflammatory stimuli induces NETs. These structures consist of a network of chromatin strands associated with predominately granule proteins, including MPO. NETs exhibit antimicrobial activity, which is proposed to augment the more-established mechanism of phagosomal killing. They may also be detrimental to the host in situations such as chronic inflammation or severe sepsis. The objective of this study was to establish whether MPO associated with NETs is active and able to kill bacteria. Neutrophils were stimulated with PMA to release NETs. Peroxidase activity measurements were performed and showed that enzymatically active MPO was released from the neutrophils, 2–4 h after stimulation, concomitant with NET formation. Approximately 30% of the total cellular MPO was released, with the majority bound to the NETs. The bound enzyme retained its activity. Staphylococcus aureus were not killed when added to preformed NETs under our assay conditions. However, addition of H2O2 to the bacteria in the presence of NETs resulted in MPO-dependent killing, which was observed with NETs in situ and with NETs when they were removed from the neutrophils by limited DNase digestion. Our results show that the enzymatic activity of MPO on NETs could contribute to antimicrobial activity or tissue injury when NETs are released from neutrophils at sites of infection or inflammation.
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Cites
Neutrophil Extracellular Traps Kill Bacteria
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y., & Zychlinsky, A. (2004) · Science · doi:10.1126/science.1092385 · Cited by 10,268
Novel cell death program leads to neutrophil extracellular traps
Fuchs, T. A., Abed, U., Goosmann, C., Hurwitz, R., Schulze, I., Wahn, V., Weinrauch, Y., Brinkmann, V., & Zychlinsky, A. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200606027 · Cited by 3,197
Extracellular DNA traps promote thrombosis
Fuchs, T. A., Brill, A., Duerschmied, D., Schatzberg, D., Monestier, M., Myers, D. D., Wrobleski, S. K., Wakefield, T. W., Hartwig, J. H., & Wagner, D. D. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1005743107 · Cited by 2,492
Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood
Clark, S. R., Ma, A. C., Tavener, S. A., McDonald, B., Goodarzi, Z., Kelly, M. M., Patel, K. D., Chakrabarti, S., McAvoy, E., Sinclair, G. D., et al. (2007) · Nature Medicine · doi:10.1038/nm1565 · Cited by 2,309
Myeloperoxidase: friend and foe
Klebanoff, S. J. (2005) · Journal of Leukocyte Biology · doi:10.1189/jlb.1204697 · Cited by 2,282
Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps
Papayannopoulos, V., Metzler, K. D., Hakkim, A., & Zychlinsky, A. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201006052 · Cited by 2,103
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Inside the Neutrophil Phagosome: Oxidants, Myeloperoxidase, and Bacterial Killing
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Self-citationNetting neutrophils in autoimmune small-vessel vasculitis
Kessenbrock, K., Krumbholz, M., Schönermarck, U., Back, W., Gross, W. L., Werb, Z., Gröne, H.-J., Brinkmann, V., & Jenne, D. E. (2009) · Nature Medicine · doi:10.1038/nm.1959 · Cited by 1,648
Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis
Hakkim, A., Fürnrohr, B. G., Amann, K., Laube, B., Abed, U. A., Brinkmann, V., Herrmann, M., Voll, R. E., & Zychlinsky, A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909927107 · Cited by 1,469
Cited by
Carr, A., & Maggini, S. (2017) · Nutrients · doi:10.3390/nu9111211 · Cited by 2,035
Neutrophil extracellular traps: Is immunity the second function of chromatin?
Brinkmann, V., & Zychlinsky, A. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201203170 · Cited by 1,021
Nauseef, W. M., & Borregaard, N. (2014) · Nature Immunology · doi:10.1038/ni.2921 · Cited by 892
Diverse novel functions of neutrophils in immunity, inflammation, and beyond
Mócsai, A. (2013) · Journal of Experimental Medicine · doi:10.1084/jem.20122220 · Cited by 674
Kolaczkowska, E., Jenne, C. N., Surewaard, B. G. J., Thanabalasuriar, A., Lee, W.-Y., Sanz, M.-J., Mowen, K., Opdenakker, G., & Kubes, P. (2015) · Nature Communications · doi:10.1038/ncomms7673 · Cited by 584
Metadata
- Authors
- Parker, H., Albrett, A. M., Kettle, A. J., & Winterbourn, C. C.
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-11-30
- Volume
- 91
- Issue
- 3
- Pages
- 369-376
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2070256030
- Cited by
- 340
- References
- 36
- FWCI
- 7.24
- Type
- journal article
- Language
- en
- Open access
- closed
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