The DOI matches its Crossref record; 96 of the 99 references it cites were found in a registry or an index.
Zebrafish as a model for the study of neutrophil biology
Abstract
To understand inflammation and immunity, we need to understand the biology of the neutrophil. Whereas these cells can readily be extracted from peripheral blood, their short lifespan makes genetic manipulations impractical. Murine knockout models have been highly informative, and new imaging techniques are allowing neutrophils to be seen during inflammation in vivo for the first time. However, there is a place for a new model of neutrophil biology, which readily permits imaging of individual neutrophils during inflammation in vivo, combined with the ease of genetic and chemical manipulation. The zebrafish has long been the model of choice for the developmental biology community, and the availability of genomic resources and tools for gene manipulation makes this an attractive model. Zebrafish innate immunity shares many features with mammalian systems, including neutrophils with morphological, biochemical, and functional features, also shared with mammalian neutrophils. Transgenic zebrafish with neutrophils specifically labeled with fluorescent proteins have been generated, and this advance has led to the adoption of zebrafish, alongside existing models, by a number of groups around the world. The use of these models has underpinned a number of key advances in the field, including the identification of a tissue gradient of hydrogen peroxide for neutrophil recruitment following tissue injury and direct evidence for reverse migration as a regulatable mechanism of inflammation resolution. In this review, we discuss the importance of zebrafish models in neutrophil biology and describe how the understanding of neutrophil biology has been advanced by the use of these models.
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“These models offer unique possibilities, e.g., to perform live imaging, due to their translucency, and genome-wide screen, due to their good genetic tractability and low cost.”
From Transition from inflammation to proliferation: a critical step during wound healing (2016) · cites this paper as [267]
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“Recently, Drosophila and zebrafish are used to investigate mechanisms of fundamental tissue repair [267, 268].”
From Transition from inflammation to proliferation: a critical step during wound healing (2016) · cites this paper as [267]
In the citing paper
“Although they have their own limitations, Drosophila and zebrafish models offer translucency for live imaging and even greater genetic tractability than mice, to study fundamental tissue repair mechanisms not possible in humans.”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [118]
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Metadata
- Authors
- Henry, K. M., Loynes, C. A., Whyte, M. K. B., & Renshaw, S. A.
- Affiliations
- Medical Research Council (GB)
- Corresponding author
- Renshaw, Stephen A
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-10-01
- Volume
- 94
- Issue
- 4
- Pages
- 633-642
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2149185484
- Cited by
- 143
- References
- 99
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 8 · 2023: 12 · 2022: 9 · 2021: 18 · 2020: 13 · 2019: 13 · 2018: 7 · 2017: 8 · 2016: 12 · 2015: 17 · 2014: 13 · 2013: 2
- FWCI
- 1.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Leukocyte Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- MRC Senior Clinical Fellowship (G0701932)
- MRC (G0700091)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- zebrafish, neutrophils, neutrophil biology, innate immunity, inflammation, transgenic zebrafish, neutrophil recruitment, reverse migration, inflammation resolution, tissue damage, genetic engineering
- MeSH terms
- Animals, Disease Models, Animal, Humans, Immunity, Neutrophils, Zebrafish, Models, Animal, Animals, Genetically Modified
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