Sequential depletion of rat testicular lipids with long-chain and very long-chain polyenoic fatty acids after X-ray-induced interruption of spermatogenesis
Open accessAbstract
When a single dose of X-rays is applied to the adult rat testis, stem spermatogonia are damaged, and spermatogenesis is interrupted. Supported by Sertoli cells, spermatogenic cells that endure irradiation complete their differentiation and gradually leave the testis as spermatozoa. In this study, the in vivo changes taking place a number of weeks after irradiation revealed cell-specific features of testicular lipid classes. A linear drop, taking about six weeks, in testis weight, nonlipid materials, free cholesterol, and 22:5n-6-rich glycerophospholipids took place with germ cell depletion. Sphingomyelins and ceramides with nonhydroxy very long-chain polyenoic fatty acids (n-VLCPUFA) disappeared in four weeks, together with the last spermatocytes, whereas species with 2-hydroxy VLCPUFA lasted for six weeks, disappearing with the last spermatids and spermatozoa. The amount per testis of 22:5n-6-rich triacylglycerols, unchanged for four weeks, fell between weeks 4 and 6, associating these lipids with spermatids and their residual bodies, detected as small, bright lipid droplets. In contrast, 22:5n-6-rich species of cholesterol esters and large lipid droplets increased in seminiferous tubules up to week 6, revealing they are Sertoli cell products. At week 30, the lipid and fatty acid profiles reflected the resulting permanent testicular involution. Our data highlight the importance of Sertoli cells in maintaining lipid homeostasis during normal spermatogenesis.
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ELOVL4 synthesizes VLC-PUFAs in the retina and testes, and it synthesizes very-long-chain saturated fatty acids (VLC-SFAs) in the skin and brain.
“ELOVL4 synthesizes VLC-PUFAs in the retina (Agbaga et al., 2010, 2008; Aveldaño, 1987) and testes (Oresti et al., 2010), and it synthesizes very-long-chain saturated fatty acids (VLC-SFAs) in the skin and brain (Cameron et al., 2007; Monroig et al., 2010).”
From Docosanoids and elovanoids from omega-3 fatty acids are pro-homeostatic modulators of inflammatory responses, cell damage and neuroprotection (2018) · cites this paper as Oresti et al., 2010
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- Authors
- Oresti, G. M., Ayuza Aresti, P. L., Gigola, G., Reyes, L. E., & Aveldaño, M. I.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Corresponding author
- Aveldaño, Marta I.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 51
- Issue
- 9
- Pages
- 2600-2610
- ISSN
- 0022-2275 (ISSN-L)
- PMC
- PMC2918443
- OpenAlex
- W2118104226
- Cited by
- 19
- References
- 29
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 1
- FWCI
- 2.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-sa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Universidad Nacional del Sur
- Agencia Nacional de Promoción Científica y Tecnológica (CONICET)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- spermatogenesis, X-ray irradiation, Sertoli cells, lipid homeostasis, germ cell depletion, cholesterol esters, lipid droplets, sphingomyelin, ceramide, triglycerides, testicular involution, residual bodies, spermatids, seminiferous tubules
- MeSH terms
- Animals, Fatty Acids, Humans, Lipids, Male, Sertoli Cells, Spermatogenesis, Spermatozoa, Testis, Rats, Wistar, Lipid Metabolism, Rats
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