Ontogeny of MMPs and TIMPs in the murine neocortex.
Open accessAbstract
Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been implicated in normal brain development, adult stroke, and, more recently, perinatal brain injury. Here, our objective was to obtain comprehensive and comparative data on the ontogeny of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the neocortex of male and female mice belonging to various strains, from embryonic life to adulthood. We used gelatin zymography, ELISA, and real-time PCR analyses. MMP-2, MMP-9, and TIMP-1 activity and/or expression peaked during embryonic life and the early neonatal period, whereas TIMP-2 peaked during the first two postnatal weeks. Comparable results were obtained in all the mouse strains except BALB/c, where MMP-2 levels were considerably lower at all ages compared with the other strains. No gender effect was observed on any of the study parameters. This comprehensive study will serve as a basis for future investigations into the role for MMPs and TIMPs in normal brain development and prenatal brain injury.
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Peak MMP-9 expression is observed during postnatal development and is reduced during adulthood.
“Peak MMP-9 expression is observed during postnatal development and is reduced during adulthood (Bednarek et al., 2009; Reinhard et al., 2015; Wen et al., 2017).”
From The Perineuronal ‘Safety’ Net? Perineuronal Net Abnormalities in Neurological Disorders (2018) · cites this paper as Bednarek et al., 2009
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- Authors
- Bednarek, N., Clément, Y., Lelièvre, V., Olivier, P., Loron, G., Garnotel, R., & Gressens, P.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Bednarek, Nathalie
- Venue
- Pediatric Research
- Published
- 2009-03
- Volume
- 65
- Issue
- 3
- Pages
- 296-300
- ISSN
- 0031-3998 (ISSN-L)
- 1530-0447
- OpenAlex
- W1982672839
- Cited by
- 35
- References
- 49
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2013: 4 · 2012: 2
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Pediatric Research (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Research Portal (King's College London) (submittedVersion, other-oa)
- Funders
- European Commission
- Institut National de la Santé et de la Recherche Médicale
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and fetal brain pathology
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Fetal and Pediatric Neurological Disorders
- Keywords
- matrix metalloproteinases, tissue inhibitors of metalloproteinases, MMP-2, MMP-9, TIMP-1, TIMP-2, neocortex, brain development, ontogeny, mouse strains, sex differences, perinatal brain injury, embryonic development, postnatal development
- MeSH terms
- Age Factors, Animals, Electrophoresis, Enzyme-Linked Immunosorbent Assay, Female, Male, Mice, Mutant Strains, Species Specificity, Gene Expression Regulation, Developmental, Neocortex, Tissue Inhibitor of Metalloproteinases, Reverse Transcriptase Polymerase Chain Reaction, Matrix Metalloproteinases, Mice
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