Cited by 35Protease and Inhibitor MechanismsNeonatal and fetal brain pathologyFetal and Pediatric Neurological Disorders

Ontogeny of MMPs and TIMPs in the murine neocortex.

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Bednarek, Nathalie, Clément, Yan, Lelièvre, Vincent, Olivier, Paul, Loron, Gauthier, Garnotel, Roselyne, Gressens, Pierre·2009·Pediatric Research

Abstract

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been implicated in normal brain development, adult stroke, and, more recently, perinatal brain injury. Here, our objective was to obtain comprehensive and comparative data on the ontogeny of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the neocortex of male and female mice belonging to various strains, from embryonic life to adulthood. We used gelatin zymography, ELISA, and real-time PCR analyses. MMP-2, MMP-9, and TIMP-1 activity and/or expression peaked during embryonic life and the early neonatal period, whereas TIMP-2 peaked during the first two postnatal weeks. Comparable results were obtained in all the mouse strains except BALB/c, where MMP-2 levels were considerably lower at all ages compared with the other strains. No gender effect was observed on any of the study parameters. This comprehensive study will serve as a basis for future investigations into the role for MMPs and TIMPs in normal brain development and prenatal brain injury.

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Authors
Bednarek, N., Clément, Y., Lelièvre, V., Olivier, P., Loron, G., Garnotel, R., & Gressens, P.
Affiliations
  • Inserm (FR)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Bednarek, Nathalie
Venue
Pediatric Research
Published
2009-03
Volume
65
Issue
3
Pages
296-300
ISSN
  • 0031-3998 (ISSN-L)
  • 1530-0447
OpenAlex
W1982672839
Cited by
35
References
49
Citations by year
2025: 2 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2013: 4 · 2012: 2
FWCI
0.67
Type
journal article
Language
en
Open access
Locations
  • Pediatric Research (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • Research Portal (King's College London) (submittedVersion, other-oa)
Funders
  • European Commission
  • Institut National de la Santé et de la Recherche Médicale
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and fetal brain pathology
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Fetal and Pediatric Neurological Disorders
Keywords
matrix metalloproteinases, tissue inhibitors of metalloproteinases, MMP-2, MMP-9, TIMP-1, TIMP-2, neocortex, brain development, ontogeny, mouse strains, sex differences, perinatal brain injury, embryonic development, postnatal development
MeSH terms
Age Factors, Animals, Electrophoresis, Enzyme-Linked Immunosorbent Assay, Female, Male, Mice, Mutant Strains, Species Specificity, Gene Expression Regulation, Developmental, Neocortex, Tissue Inhibitor of Metalloproteinases, Reverse Transcriptase Polymerase Chain Reaction, Matrix Metalloproteinases, Mice

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