Glucocorticoid receptor gene expression in the exocrine and endocrine rat pancreas
Abstract
Although pleiotropic effects of glucocorticoids on the endocrine and exocrine pancreas are well documented, it remains controversial whether these effects are due to direct interactions of glucocorticoid hormones with their receptors in the respective target cells. We therefore examined gene expression of the glucocorticoid receptor (GR) in various functional compartments of the rat pancreas. Using in situ hybridization by generating a radioactive cRNA probe from a specific cDNA clone for the rat glucocorticoid receptor we found a gradient in the expression of receptor mRNA transcripts from endocrine > acinar > ductal cells. In contrast, no specific hybridization signal was observed in the endothelial cells of vascular structures. Using a monospecific polyclonal antibody against the rat glucocorticoid receptor in Western blot analyses we found significant expression of the 94 kD receptor protein in an enriched pancreatic acinar cell preparation. This data suggest that rat pancreatic exocrine cells might serve a direct target tissue for glucocorticoid action.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Glucocorticoid receptors are present on β-cells.
“As GC receptors are known to be present on â-cells,29,30 it is possible that GCs may also exert direct stimulatory and inhibitory effects on insulin secretion.”
From Twenty‐four‐hour profiles of plasma glucose, insulin, C‐peptide and free fatty acid in subjects with varying degrees of glucose tolerance following short‐term, medium‐dose prednisone (20 mg/day) treatment: evidence for differing effects on insulin secretion and action (2012) · cites this paper as [30]
Cited by
Sorted by citations
Lambillotte, C., Gilon, P., & Henquin, J. C. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119175 · Cited by 419 · see citing works
von Marschall, Z., Cramer, T., Höcker, M. R., Burde, R., Plath, T., Schirner, M., Heidenreich, R., Breier, G., Riecken, E., Wiedenmann, B., et al. (2000) · Gastroenterology · doi:10.1053/gast.2000.19578 · Cited by 189 · see citing works
Speirs, H., Seckl, & Brown, R. (2004) · Journal of Endocrinology · doi:10.1677/joe.0.1810105 · Cited by 138 · see citing works
Roussel, R., Reis, A. F., Dubois‐Laforgue, D., Bellanné‐Chantelot, C., Timsit, J., & Velho, G. (2003) · Clinical Endocrinology · doi:10.1046/j.1365-2265.2003.01831.x · Cited by 72 · see citing works
Yuen, K. C. J., McDaniel, P. A., & Riddle, M. C. (2012) · Clinical Endocrinology · doi:10.1111/j.1365-2265.2011.04242.x · Cited by 58 · see citing works
Hill, D., Hogg, J., Petrik, J., Arany, E. J., & Han, V. (1999) · Journal of Endocrinology · doi:10.1677/joe.0.1600305 · Cited by 55 · see citing works
Laudet, V., & Gronemeyer, H. (2002) · Elsevier eBooks · doi:10.1016/b978-012437735-6/50032-1 · Cited by 50 · see citing works
Stressing diabetes? The hidden links between insulinotropic peptides and the HPA axis
Diz-Chaves, Y., Gil‐Lozano, M., Toba, L., Fandiño, J., Ogando, H., González-Matías, L. C., & Mallo, F. (2016) · Journal of Endocrinology · doi:10.1530/joe-16-0118 · Cited by 44 · see citing works
Steroid effects on intracellular degradation of insulin and crinophagy in isolated pancreatic islets
Sandberg, M., & Borg, L. (2007) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2007.07.007 · Cited by 18 · see citing works
Bromocriptine-Induced Hyperglycemia in Nonobese Diabetic Mice: Kinetics and Mechanisms of Action
Durant, S., Coulaud, J., & Homo‐Delarche, F. (2007) · The Review of Diabetic Studies · doi:10.1900/rds.2007.4.185 · Cited by 17 · see citing works
Fetal programming of the pancreatic β cells and the implications for postnatal diabetes
Hill, D. J. (1999) · Seminars in Neonatology · doi:10.1016/s1084-2756(99)80012-8 · Cited by 9 · see citing works
Proteins Expressed by Pancreatic Duct Cellsand Their Relatives
Hollingsworth, M. A. (1999) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1999.tb09508.x · Cited by 8 · see citing works
Genetic contributions to glucose intolerance in polycystic ovary syndrome
Ehrmann, D. A. (2004) · Reproductive BioMedicine Online · doi:10.1016/s1472-6483(10)62106-2 · Cited by 7 · see citing works
Molecular Aspects of Insulin Signaling
Emkey, R., & Kahn, C. R. (2001) · Comprehensive physiology · doi:10.1002/cphy.cp070212 · Cited by 1 · see citing works
- Authors
- Matthes, H.
- ORCID
- Matthes, H.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1994-07-01
- Volume
- 135
- Issue
- 1
- Pages
- 476-479
- ISSN
- 0013-7227 (ISSN-L)
- OpenAlex
- W2092249523
- Cited by
- 28
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2016: 1 · 2012: 1
- FWCI
- 0.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- glucocorticoid receptor, gene expression, pancreas, exocrine pancreas, endocrine pancreas, acinar cells, pancreatic islets, glucocorticoid activity, receptor mRNA, receptor proteins
- MeSH terms
- Animals, Islets of Langerhans, Male, Pancreas, Receptors, Glucocorticoid, RNA, Messenger, Transcription, Genetic, Gene Expression, Rats, Sprague-Dawley, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
