Positive and negative regulation of the rat vasopressin gene promoter
Abstract
To study the transcriptional regulation of the vasopressin gene in vitro, 3 kb of the 5' regulatory region of the rat vasopressin gene was isolated and subcloned, along with a series of various deletion mutants, into vectors containing the luciferase reporter gene. After transfecting these genes transiently into the human choriocarcinoma cell line JEG-3 along with a glucocorticoid receptor (GR) expression vector, transcriptional activity was quantitated using the luciferase assay. Forskolin, 8-bromo-cAMP, and protein kinase A catalytic subunit expression all markedly increased transcription from the 3-kb promoter. Analyses with deletion mutants of the promoter showed that two cAMP-responsive element (CRE)-like sequences (-227 to -220 bp and -123 to -116 bp) contribute to this positive regulation. Expression of KCREB, a dominant negative mutant of the cAMP-responsive element binding protein (CREB), suggested the involvement of CREB. Transfection of the activator protein 2 (AP2) DNA consensus sequence partially blocked transcription. Dexamethasone suppressed forskolin-stimulated expression. The negative effect of glucocorticoid was GR dependent and may be mediated by a mechanism not involving GR binding to DNA because it was independent of the putative glucocorticoid-responsive element previously reported in the vasopressin promoter (-622 to -608 bp) and was preserved in the shorter promoter constructs in which no glucocorticoid-responsive element-like sequence was found. Our d…
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CREB binds to the AVP promoter region to activate gene transcription in vitro.
“Indeed, studies have shown that CREB binds to the AVP promoter region to activate gene transcription in vitro.46”
From Neonatal MeCP2 is important for the organization of sex differences in vasopressin expression (2012) · cites this paper as [46]
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Sausville, E., Carney, D., & Battey, J. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39236-0 · Cited by 338 · see citing works
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Oxytocin and vasopressin: genetics and behavioral implications
Caldwell, H. K., & Young, W. S. (2006) · Handbook of Neurochemistry and Molecular Neurobiology · doi:10.1007/978-0-387-30381-9_25 · Cited by 113 · see citing works
Nováková, M., Sládek, M., & Sumová, A. (2010) · Journal of Biological Rhythms · doi:10.1177/0748730410377967 · Cited by 50 · see citing works
Neonatal MeCP2 is important for the organization of sex differences in vasopressin expression
Forbes-Lorman, R. M., Rautio, J. J., Kurian, J. R., Auger, A. P., & Auger, C. J. (2012) · Epigenetics · doi:10.4161/epi.7.3.19265 · Cited by 43 · see citing works
- Authors
- Iwasaki, Y.
- Affiliations
- Boston Children's Hospital (US)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1997-12-01
- Volume
- 138
- Issue
- 12
- Pages
- 5266-5274
- ISSN
- 0013-7227 (ISSN-L)
- OpenAlex
- W2020163395
- Cited by
- 108
- References
- 1
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 3 · 2016: 3 · 2015: 7 · 2014: 3 · 2012: 5
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns029384-03)
- National Institute of Neurological Disorders and Stroke (R01-NS-29384)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
- Keywords
- vasopressin gene, promoter regulation, transcriptional regulation, cAMP response element, CREB, activator protein-2, glucocorticoid receptor, dexamethasone, forskolin, protein kinase A, trans-acting factors, rats, JEG-3 cells, luciferase reporter
- MeSH terms
- Animals, Base Sequence, Dexamethasone, Colforsin, Gene Expression Regulation, Glucocorticoids, Humans, Molecular Sequence Data, Promoter Regions, Genetic, Protein Kinase C, Tumor Cells, Cultured, Vasopressins, Gene Deletion, Cyclic AMP Response Element-Binding Protein, Cyclic AMP-Dependent Protein Kinases, Rats
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