The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
Calcitonin Plays a Critical Role in Regulating Skeletal Mineral Metabolism during Lactation
Abstract
The maternal skeleton rapidly demineralizes during lactation to provide calcium to milk, responding to the stimuli of estrogen deficiency and mammary-secreted PTH-related protein. We used calcitonin/calcitonin gene-related peptide-alpha (Ctcgrp) null mice to determine whether calcitonin also modulates lactational mineral metabolism. During 21 d of lactation, spine bone mineral content dropped 53.6% in Ctcgrp nulls vs. 23.6% in wild-type (WT) siblings (P < 0.0002). After weaning, bone mineral content returned fully to baseline in 18.1 d in Ctcgrp null vs. 13.1 d in WT (P < 0.01) mice. Daily treatment with salmon calcitonin from the onset of lactation normalized the losses in Ctcgrp null mice, whereas calcitonin gene-related peptide-alpha or vehicle was without effect. Compared with WT, Ctcgrp null mice had increased circulating levels of PTH and up-regulation of mammary gland PTH-related protein mRNA. In addition, lactation caused the Ctcgrp null skeleton to undergo more trabecular thinning and increased trabecular separation compared with WT. Our studies confirm that an important physiological role of calcitonin is to protect the maternal skeleton against excessive resorption and attendant fragility during lactation and reveal that the postweaning skeleton has the remarkable ability to rapidly recover even from losses of over 50% of skeletal mineral content.
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“Injection of sCT normalized the bone parameters, whereas CGRP was without effect, indicating that calcitonin is the peptide that plays an important role in calcium balance and preservation of the maternal skeleton during lactation and its recovery after weaning (222).”
From The Activity of Peptides of the Calcitonin Family in Bone (2019) · cites this paper as [222]
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“Thirteen days after weaning, spine bone mineral content returned to baseline values in the WT mice, whereas in the Calca KO mice, it took 18 days to reach these values.”
From The Activity of Peptides of the Calcitonin Family in Bone (2019) · cites this paper as [222]
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“In the Calca KO model, deficient in both calcitonin and CGRP, spine bone mineral content dropped during 21 days of lactation by over 50%, whereas in the WT controls, the drop was only of 23.6% (222).”
From The Activity of Peptides of the Calcitonin Family in Bone (2019) · cites this paper as [222]
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Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Use of Inhaled Corticosteroids and Risk of Fractures
van Staa, T. P., Leufkens, H. G. M., & Cooper, C. C. (2001) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2001.16.3.581 · Cited by 1,297
Use of oral corticosteroids and risk of fractures
Van Staa, T. P., Leufkens, H. G. M., Abenhaim, L., Zhang, B., & Cooper, C. (2000) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2000.15.6.993 · Cited by 1,263
Maternal-Fetal Calcium and Bone Metabolism During Pregnancy, Puerperium, and Lactation
Kovács, C. S., & Kronenberg, H. M. (1997) · Endocrine Reviews · doi:10.1210/edrv.18.6.0319 · Cited by 818
Treatment of Postmenopausal Osteoporosis with Transdermal Estrogen
Lufkin, E. G., Wahner, H. W., O’Fallon, W. M., Hodgson, S. F., Kotowicz, M. A., Lane, A. W., Judd, H. L., Caplan, R. H., & Riggs, B. L. (1992) · Annals of Internal Medicine · doi:10.7326/0003-4819-117-1-1 · Cited by 813
Evidence for Calcitonin—A New Hormone from the Parathyroid That Lowers Blood Calcium
COPP, D. H., CAMERON, E. C., CHENEY, B. A., DAVIDSON, A. G. F., & HENZE, K. G. (1962) · Endocrinology · doi:10.1210/endo-70-5-638 · Cited by 697
Hormonal Regulation of Mammary Differentiation and Milk Secretion
Neville, M. C., McFadden, T. B., & Forsyth, I. A. (2002) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1023/a:1015770423167 · Cited by 628
Transport of Milk Constituents by the Mammary Gland
SHENNAN, D. B., & Peaker, M. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.3.925 · Cited by 413
Changes in Bone Density With Lactation
Sowers, M. R. (1993) · JAMA · doi:10.1001/jama.1993.03500240074029 · Cited by 373
Calcium and Bone Metabolism in Pregnancy and Lactation*
Kovács, C. S. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.86.6.7575 · Cited by 312
Zhuang, L., Peng, J., Tou, L., Takanaga, H., Adam, R. M., Hediger, M. A., & Freeman, M. R. (2002) · Laboratory Investigation · doi:10.1097/01.lab.0000043910.41414.e7 · Cited by 304
Effects of 1- and 6-month spaceflight on bone mass and biochemistry in two humans
Collet, P., Uebelhart, D., Vico, L., Moro, L., Hartmann, D. J., Roth, M., & Alexandre, C. (1997) · Bone · doi:10.1016/s8756-3282(97)00052-5 · Cited by 288
Grill, V., Ho, P. M. W., Body, J., JOHANSON, N., LEE, S. C., Kukreja, S. C., Moseley, J. M., & Martin, T. J. (1991) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-73-6-1309 · Cited by 287
Hypocalcemia in Dairy Cows: Meta-analysis and Dietary Cation Anion Difference Theory Revisited
Lean, I. J., DeGaris, P. J., McNeil, D. M., & Block, E. (2006) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(06)72130-0 · Cited by 272
Elevated Parathyroid Hormone-Related Peptide Associated With Lactation and Bone Density Loss
Sowers, M. F. (1996) · JAMA · doi:10.1001/jama.1996.03540070045029 · Cited by 259
Calcium and Bone Metabolism During Pregnancy and Lactation
Kovács, C. S. (2005) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-005-5394-0 · Cited by 251
Neville, M. C., Keller, R. P., Seacat, J. M., Casey, C. E., Allen, J. C., & Archer, P. (1984) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/40.3.635 · Cited by 244
VanHouten, J. N., Dann, P. R., McGeoch, G., Brown, E. M., Krapcho, K. J., Neville, M. C., & Wysolmerski, J. J. (2004) · Journal of Clinical Investigation · doi:10.1172/jci200418776 · Cited by 213
Increased bone mass is an unexpected phenotype associated with deletion of the calcitonin gene
Hoff, A. O., Catala-Lehnen, P., Thomas, P. M., Priemel, M., Rueger, J. M., Nasonkin, I., Bradley, A., Hughes, M. R., Ordonez, N., Cote, G. J., et al. (2002) · Journal of Clinical Investigation · doi:10.1172/jci200214218 · Cited by 209
Self-citationSong, Y., Fleet, J. C., & Shigeaki, K. A. T. O. (2003) · Journal of Nutrition · doi:10.1093/jn/133.2.374 · Cited by 198
Endocrinology of milk production
Svennersten‐Sjaunja, K., & Olsson, K. (2005) · Domestic Animal Endocrinology · doi:10.1016/j.domaniend.2005.03.006 · Cited by 188
Bone mineral density and bone turnover before and after surgical cure of Cushing's syndrome.
Hermus, A., Smals, A., Swinkels, L., Huysmans, D., Pieters, G., Sweep, C., Corstens, F., & Kloppenborg, P. (1995) · Journal of Clinical Endocrinology and Metabolism · doi:10.1210/jcem.80.10.7559865 · Cited by 181
VanHouten, J. N., Dann, P. R., Stewart, A. F., Watson, C. J., Pollak, M., Karaplis, A. C., & Wysolmerski, J. J. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319504 · Cited by 169
Long-term follow-up of Skylab bone demineralization.
Tilton, F. E., Degioanni, J. J., & Schneider, V. S. (1980) · PubMed · Cited by 154
VanHouten, J. N., & Wysolmerski, J. J. (2003) · Endocrinology · doi:10.1210/en.2003-0892 · Cited by 154
Calcium and Bone Disorders During Pregnancy and Lactation
Kovács, C. S., & Fuleihan, G. E. (2006) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2005.09.004 · Cited by 153
VanHouten, J. N., Dann, P. R., Stewart, A. F., Watson, C. J., Pollak, M., Karaplis, A. C., & Wysolmerski, J. J. (2003) · Journal of Clinical Investigation · doi:10.1172/jci19504 · Cited by 151
Zinser, G. M., & Welsh, J. (2004) · Molecular Endocrinology · doi:10.1210/me.2003-0469 · Cited by 145
Thiede, M. A. (1989) · Molecular Endocrinology · doi:10.1210/mend-3-9-1443 · Cited by 143
Defective bone mineralization and osteopenia in young adult FGFR3-/- mice.
Valverde-Franco, G., Liu, H., Davidson, D., Chai, S., Valderrama-Carvajal, H., Goltzman, D., Ornitz, D., & Henderson, J. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddh034 · Cited by 143
Calcium and Bone Metabolism in Pregnancy and Lactation
Kovács, C. S. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.86.6.2344 · Cited by 137
Kovács, C. S., Woodland, M. L., Fudge, N. J., & Friel, J. K. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00354.2004 · Cited by 136
Self-citationHorst, R. L., Goff, J. P., & Reinhardt, T. A. (2005) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-005-5397-x · Cited by 131
Management of Paget's disease of bone
Langston, A. L. (2004) · Rheumatology · doi:10.1093/rheumatology/keh243 · Cited by 124
Dobnig, H., Kainer, F., Stepan, V. M., Winter, R., Lipp, R. W., Schaffer, M., Kahr, A., Nocnik, S., Patterer, G., & Leb, G. (1995) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.80.12.8530622 · Cited by 122
Hurley, D. L., Tiegs, R. D., Wahner, H. W., & Heath, H. (1987) · New England Journal of Medicine · doi:10.1056/nejm198708273170904 · Cited by 118
Epithelial Ca2+ entry channels: transcellular Ca2+ transport and beyond
Peng, J., Brown, E. M., & Hediger, M. A. (2003) · The Journal of Physiology · doi:10.1111/j.1469-7793.2003.00729.x · Cited by 114
Buhimschi, C. S. (2004) · Obstetrics and Gynecology Clinics of North America · doi:10.1016/j.ogc.2004.08.002 · Cited by 107
Perspective on the Impact of Weightlessness on Calcium and Bone Metabolism
Holick, M. F. (1998) · Bone · doi:10.1016/s8756-3282(98)00014-3 · Cited by 103
The Evolutionary Origins of Maternal Calcium and Bone Metabolism During Lactation
Wysolmerski, J. J. (2002) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1023/a:1022800716196 · Cited by 81
Production of parathyroid hormone-related protein by the mammary gland of the goat
RATCLIFFE, W. A., Thompson, G. E., Caret, A. D., & Peaker, M. (1992) · Journal of Endocrinology · doi:10.1677/joe.0.1330087 · Cited by 76
Kovács, C. S., Chafe, L. L., Woodland, M. L., McDonald, K. R., Fudge, N. J., & Wookey, P. J. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00369.2001 · Cited by 70
Self-citationKovács, C. S., & Chik, C. L. (1995) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.80.10.7559893 · Cited by 65
Changes in bone density with lactation
Sowers, M. (1993) · JAMA · doi:10.1001/jama.269.24.3130 · Cited by 62
Bucht, E., Telenius‐Berg, M., Lundell, G. E. F., & Sjöberg, H. (1986) · European Journal of Endocrinology · doi:10.1530/acta.0.1130529 · Cited by 59
Skeletal adaptations during mammalian reproduction.
Bowman, B. M., & Miller, S. C. (2001) · PubMed · Cited by 57
Changes in specific nutrients in breast milk during extended lactation
Karra, M. V., Udipi, S. A., Kirksey, A., & ROEPKE, J. B. (1986) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/43.4.495 · Cited by 54
Current Management Strategies for Hypercalcemia
Pecherstorfer, M., Brenner, K., & Zojer, N. (2003) · Treatments in Endocrinology · doi:10.2165/00024677-200302040-00005 · Cited by 54
Peng, T., Garner, S. C., Kusy, R. P., & Hirsch, P. F. (1988) · PubMed · Cited by 54
Stiegler, C., Leb, G., Kleinert, R., Warnkross, H., Ramschak-Schwarzer, S., Lipp, R. W., Clarici, G., Krejs, G. J., & Dobnig, H. (1995) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650100512 · Cited by 50
Bone Density and Mineral Metabolism in Thyroidectomized Patients Treated with Long-Term l -Thyroxine
Giannini, S., Nobile, M., Sartori, L., Binotto, P., Ciuffreda, M., Gemo, G., Pelizzo, M. R., D’Angelo, A., & Crepaldi, G. (1994) · Clinical Science · doi:10.1042/cs0870593 · Cited by 49
Garner, S. C., Peng, T., Hirsch, P. F., Boass, A., & Toverud, S. U. (1987) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650020412 · Cited by 44
The expression of the calcitonin receptor gene in the brain and pituitary gland of the rat
Sheward, W. J., Lutz, E. M., & Harmar, A. J. (1994) · Neuroscience Letters · doi:10.1016/0304-3940(94)90553-3 · Cited by 43
Elevated parathyroid hormone-related peptide associated with lactation and bone density loss
Sowers, M. F. (1996) · JAMA · doi:10.1001/jama.276.7.549 · Cited by 42
ROLE OF CALCITONIN IN PROTECTING THE SKELETON DURING PREGNANCY AND LACTATION
Taylor, T. G., LEWIS, P. E., & BALDERSTONE, O. (1975) · Journal of Endocrinology · doi:10.1677/joe.0.0660297 · Cited by 36
Induction of Calcitonin and Calcitonin Receptor Expression in Rat Mammary Tissue during Pregnancy1
Tverberg, L. A., Gustafson, M. F., Scott, T. L., Arzumanova, I. V., Provost, E., Yan, A. W., & Rawie, S. A. (2000) · Endocrinology · doi:10.1210/endo.141.10.7712 · Cited by 35
González, D., Mautalén, C., Correa, P. H., Tamer, E. E., & El Tamer, S. (1991) · European Journal of Endocrinology · doi:10.1530/acta.0.1240521 · Cited by 34
Calcitonin Secretion by Human Placental Tissue
Balabanova, S., Kruse, B., & Wolf, A. (1987) · Acta Obstetricia Et Gynecologica Scandinavica · doi:10.3109/00016348709103646 · Cited by 34
Fractures After Thyroidectomy in Men: A Population-Based Cohort Study
Nguyen, T. T., Heath, H., Bryant, S. C., O'Fallon, W. M., & Melton, L. J. (1997) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1997.12.7.1092 · Cited by 31
Ren, Y., CHIEN, J. R., Yp, S., & Gv, S. (2001) · Journal of Endocrinology · doi:10.1677/joe.0.1710217 · Cited by 31
Rabbani, S. A., Khalili, P., Arakelian, A., Pizzi, H., Chen, G., & Goltzman, D. (2005) · Endocrinology · doi:10.1210/en.2005-0062 · Cited by 30
Kovacs, C. S. (1995) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.80.10.3036 · Cited by 30
Self-citationGarner, S. C., Peng, T., & Toverud, S. U. (1988) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650030311 · Cited by 27
Lewis, P., Rafferty, B., Shelley, M., & Cj, R. (1971) · PubMed · Cited by 26
Parathyroid Hormone-Related Protein
Nissenson, R. A. (2000) · Reviews in Endocrine and Metabolic Disorders · doi:10.1023/a:1026578720736 · Cited by 23
Calcitonin Inhibition of Prolactin Secretion in Lactating Rats: Mechanism of Action
Tohei, A., VandeGarde, B., Arbogast, L. A., & Voogt, J. L. (2000) · Neuroendocrinology · doi:10.1159/000054553 · Cited by 16
Induction of Calcitonin and Calcitonin Receptor Expression in Rat Mammary Tissue during Pregnancy
Tverberg, L. A. (2000) · Endocrinology · doi:10.1210/en.141.10.3696 · Cited by 16
Yuan, R., Kulkarni, T., Wei, F., & Shah, G. V. (2004) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2004.06.013 · Cited by 15
Reduced bone mass in calcitonin-deficient rats whether lactating or not
Hirsch, P. F., & Hagaman, J. R. (1986) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650010206 · Cited by 14
Possible effect of calcitonin deficiency on bone mass after subtotal thyroidectomy.
Mirzaei, S., Krotla, G., Knoll, P., Koriska, K., & Köhn, H. D. (1999) · PubMed · Cited by 14
Parathyroid Hormone-Related Protein
Rubin, L. P., & Torday, J. S. (2003) · Humana Press eBooks · doi:10.1385/1-59259-014-4:269 · Cited by 7
Research works printed but not linked: 4
- [16]16. Deftos LJ 2003 Calcitonin. In: Favus MJ, ed. Primer on the metabolic bone diseases and disorders of mineral metabolism. 5th ed. Washington, DC: ASBMR Press; 137-141
- [19]19. Barlet JP, Garel JM 1975 Physiological role of calcitonin in pregnant goats and ewes. In: Talmage RV, Owen M, Parsons A, eds. Calcium regulating hormones: Proc Fifth Parathyroid Conference, Oxford, UK, 1974. 1st ed. Amsterdam: Excerpta Medica; 119 -121
- [39]39. Ju ¨ppner H, Kronenberg HM 2003 Parathyroid hormone. In: Favus MJ, ed. Primer on the metabolic bone diseases and disorders of mineral metabolism. 5th ed. Washington, DC: ASBMR Press; 117-124
- [66]66. Kovacs CS, Kronenberg HM 2003 Skeletal physiology: pregnancy and lacta- tion. In: Favus MJ, ed. Primer on the metabolic bone diseases and disorders of mineral metabolism. 5th ed. Washington, DC: ASBMR Press; 80 -86 Endocrinology is published monthly by The Endocrine Society (http://www.endo-society.org), the foremost professional society serving the endocrine community.
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Metadata
- Authors
- Woodrow, J. P., Sharpe, C. J., Fudge, N. J., Hoff, A. O., Gagel, R. F., & Kovacs, C. S.
- Affiliations
- Memorial University of Newfoundland (CA)
- The University of Texas MD Anderson Cancer Center (US)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2006-09
- Volume
- 147
- Issue
- 9
- Pages
- 4010-4021
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2038783196
- Cited by
- 195
- References
- 70
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 13 · 2023: 6 · 2022: 5 · 2021: 12 · 2020: 14 · 2019: 11 · 2018: 7 · 2017: 16 · 2016: 9 · 2015: 7 · 2014: 10 · 2013: 9 · 2012: 16
- FWCI
- 10.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Society for Endocrinology
- Memorial University of Newfoundland
- Canadian Institutes of Health Research
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- calcitonin, lactation, bone demineralization, salmon calcitonin, PTH-related protein, parathyroid hormone, estrogen deficiency, trabecular bone, bone mineral content
- MeSH terms
- Animals, Biomechanical Phenomena, Bone and Bones, Bone Diseases, Metabolic, Bone Resorption, Calcitonin, Calcium, Duodenum, Estradiol, Female, Intestinal Absorption, Lactation, Mammary Glands, Animal, Milk, Minerals, Parathyroid Hormone, Pregnancy, RNA, Messenger, Bone Density, Calcitonin Gene-Related Peptide, Mice, Knockout, Parathyroid Hormone-Related Protein, Mice
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