The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Prolactin Regulates Kisspeptin Neurons in the Arcuate Nucleus to Suppress LH Secretion in Female Rats
Abstract
Prolactin (PRL) is known to suppress LH secretion. Kisspeptin neurons regulate LH secretion and express PRL receptors. We investigated whether PRL acts on kisspeptin neurons to suppress LH secretion in lactating (Lac) and virgin rats. Lac rats displayed high PRL secretion and reduced plasma LH and kisspeptin immunoreactivity in the arcuate nucleus (ARC). Bromocriptine-induced PRL blockade significantly increased ARC kisspeptin and plasma LH levels in Lac rats but did not restore them to the levels of non-Lac rats. Bromocriptine effects were prevented by the coadministration of ovine PRL (oPRL). Virgin ovariectomized (OVX) rats treated with either systemic or intracerebroventricular oPRL displayed reduction of kisspeptin expression in the ARC and plasma LH levels, and these effects were comparable with those of estradiol treatment in OVX rats. Conversely, estradiol-treated OVX rats displayed increased kisspeptin immunoreactivity in the anteroventral periventricular nucleus, whereas oPRL had no effect in this brain area. The expression of phosphorylated signal transducer and activator of transcription 5 was used to determine whether kisspeptin neurons in the ARC were responsive to PRL. Accordingly, intracerebroventricular oPRL induced expression of phosphorylated signal transducer and activator of transcription 5 in the great majority of ARC kisspeptin neurons in virgin and Lac rats. We provide here evidence that PRL acts on ARC neurons to inhibit kisspeptin expression in female rats. During lactation, PRL contributes to the inhibition of ARC kisspeptin. In OVX rats, high PRL levels suppress kisspeptin expression and reduce LH release. These findings suggest a pathway through which hyperprolactinemia may inhibit LH secretion and thereby cause infertility.
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“In addition, during lactation, elevated PRL inhibits kisspeptin expression in the arcuate nucleus (35).”
From Kisspeptin Overcomes GnRH Neuronal Suppression Secondary to Hyperprolactinemia in Humans (2022) · cites this paper as [35]
Citations
Cites
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A role for kisspeptins in the regulation of gonadotropin secretion in the mouse
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Regulation of Kiss1 gene expression in the brain of the female mouse
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Increased hypothalamic GPR54 signaling: A potential mechanism for initiation of puberty in primates
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Kisspeptins and Reproduction: Physiological Roles and Regulatory Mechanisms
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Kisspeptin neurons in the arcuate nucleus of the ewe express both dynorphin A and neurokinin B
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Control of puberty onset and fertility by gonadotropin-releasing hormone neurons
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60 YEARS OF NEUROENDOCRINOLOGY: The hypothalamo-prolactin axis
Grattan, D. R. (2015) · Journal of Endocrinology · doi:10.1530/joe-15-0213 · Cited by 379
“…reduced in the arcuate nucleus of lactating rats associated with the suppression of pulsatile GnRH secretion during lactation (Yamada et al. 2007, 2012, Smith et al. 2010, True et al. 2011, Araujo-Lopes et al. 2014, Ladyman & Woodside 2014), and in both populations in the mouse (Brown et al. 2014).”
New insights in prolactin: pathological implications
Bernard, V., Young, J., Chanson, P., & Binart, N. (2015) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2015.36 · Cited by 244
The Role of Kisspeptin in the Control of the Hypothalamic-Pituitary-Gonadal Axis and Reproduction
Xie, Q., Kang, Y., Zhang, C., Ye, X., Wang, C., Liu, J., Yu, C., Zhao, H., & Huang, D. (2022) · Frontiers in Endocrinology · doi:10.3389/fendo.2022.925206 · Cited by 257
Moore, A. M., Coolen, L. M., Porter, D. T., Goodman, R. L., & Lehman, M. N. (2018) · Endocrinology · doi:10.1210/en.2018-00389 · Cited by 190
Metadata
- Authors
- Araujo-Lopes, R., Crampton, J. R., Aquino, N. S. S., Miranda, R. M., Kokay, I. C., Reis, A. M., Franci, C. R., Grattan, D. R., & Szawka, R. E.
- Affiliations
- University of Otago (NZ)
- Universidade Federal de Minas Gerais (BR)
- Universidade de São Paulo (BR)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2014-03
- Volume
- 155
- Issue
- 3
- Pages
- 1010-1020
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2111299253
- Cited by
- 119
- References
- 58
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 9 · 2023: 6 · 2022: 15 · 2021: 10 · 2020: 15 · 2019: 11 · 2018: 7 · 2017: 9 · 2016: 10 · 2015: 11 · 2014: 6
- FWCI
- 14.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Pfizer
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
- Keywords
- prolactin, kisspeptin neurons, arcuate nucleus, luteinizing hormone secretion, lactation, hyperprolactinemia, ovariectomy, estradiol, anteroventral periventricular nucleus, STAT5 phosphorylation, bromocriptine, prolactin receptor, female infertility, hypothalamic regulation of reproduction
- MeSH terms
- Animals, Arcuate Nucleus of Hypothalamus, Bromocriptine, Estradiol, Female, Hyperprolactinemia, Immunohistochemistry, Luteinizing Hormone, Neurons, Phosphorylation, Prolactin, Radioimmunoassay, Sheep, Rats, Sprague-Dawley, Rats, Wistar, STAT5 Transcription Factor, Rats, Kisspeptins
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