The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Purification and Characterization of the Corticosteroid 1lβ-Dehydrogenase Component of the Rat Liver 1lβ-Hydroxysteroid Dehydrogenase Complex*
Abstract
We have proposed that 11 beta-hydroxysteroid dehydrogenase is composed of structurally independent units with 11 beta-dehydrogenase and 11-reductase activities. We now report the purification of rat liver 11 beta-dehydrogenase to apparent homogeneity. Starting with microsomes, 800-fold purification was achieved with agarose-NADP affinity chromatography. No 11-reductase accompanied the purification. Homogeneity of 11 beta-dehydrogenase was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid end-group analysis and immunoprecipitation. The terminal amino acid was methionine. Monomer mol wt was 34,000. The enzyme was found to be a glycoprotein. A sequence of 40 amino acid units was identified from the amino end. The amino-terminal region was found to be highly nonpolar. Unlike unpurified microsomal 11 beta-dehydrogenase, which showed curvilinear Eadie plots, homogeneous enzyme gave rectilinear plots. Michaelis constants were 1.83 +/- 0.06 microM for corticosterone and 17.3 +/- 2.24 microM for cortisol. First order rate constants were 10 times greater for corticosterone than cortisol, and maximum velocities were similar.
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Cited by
11β-Hydroxysteroid dehydrogenase type 1: a tissue specific regulator of glucocorticoid response
Tomlinson, J. W., Walker, E. A., Bujalska, I. J., Draper, N., Lavery, G. G., Cooper, M. S., Hewison, M., & Stewart, P. M. (2004) · Endocrine Reviews · doi:10.1210/er.2003-0031 · Cited by 969
11β-hydroxysteroid dehydrogenases: Intracellular gate-keepers of tissue glucocorticoid action
Chapman, K., Holmes, M., & Seckl, J. (2013) · Physiological Reviews · doi:10.1152/physrev.00020.2012 · Cited by 887
11β-Hydroxysteroid Dehydrogenase
Stewart, P. M., & Krozowski, Z. S. (1997) · Vitamins & Hormones · doi:10.1016/s0083-6729(08)60646-9 · Cited by 718
Human hypertension caused by mutations in the kidney isozyme of 11β–hydroxysteroid dehydrogenase
Mune, T., Rogerson, F. M., Nikkilä, H., Agarwal, A. K., & White, P. C. (1995) · Nature Genetics · doi:10.1038/ng0895-394 · Cited by 619
Prenatal stress, glucocorticoids and the programming of the brain
Welberg, L. A. M., & Seckl, J. R. (2001) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2001.00601.x · Cited by 928
Metadata
- Authors
- LAKSHMI, V., & MONDER, C.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1988-11
- Volume
- 123
- Issue
- 5
- Pages
- 2390-2398
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W1994533492
- Cited by
- 311
- References
- 21
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- LAKSHMI, V., & MONDER, C. (1988). Purification and Characterization of the Corticosteroid 1lβ-Dehydrogenase Component of the Rat Liver 1lβ-Hydroxysteroid Dehydrogenase Complex*. Endocrinology. https://doi.org/10.1210/endo-123-5-2390
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