Parathyroid Hormone and Calcium: Interactions in the Control of Renin Secretion in the Isolated, Nonfiltering Rat Kidney*
Abstract
The present study was designed to characterize the interaction of calcium and PTH in the control of renin release in isolated rat kidneys perfused in a closed circuit at constant flow. Kidneys were rendered nonfiltering using low perfusion pressures (70 mm Hg) and a hyperoncotic perfusate (100 g/liter BSA). Under these conditions, differences in perfusion pressure were less than 9 mm Hg between control and PTH-treated kidneys over the 50 min of perfusion. In the absence of PTH, renin release was inversely correlated with ionized calcium (Ca2+) concentration, with the highest release of renin noted with 1 mM EGTA and no added calcium. Also, verapamil treatment markedly elevated renin release, even in the presence of 2 mM Ca2+. In contrast, renin secretion was strongly depressed by 20 nM BAY-K8644 in the perfusate. In medium containing normal calcium concentrations (1 mM Ca2+), rat PTH(1-34) induced a 2-fold greater renin accumulation than in the control, non-PTH-treated kidneys. Isoproterenol induced a 5-fold stimulation under the same conditions. In the 0 Ca2+/1 mM EGTA perfusion, PTH did not elevate renin secretion. Renin release in response to PTH in 2 mM Ca2+ was similar to that observed in the 1 mM Ca2+ perfusion. PTH also reversed the effects of BAY-K8644 to suppress renin release. In verapamil-treated kidneys, PTH failed to stimulate renin release. These results indicate that PTH stimulates renin release by a process independent of the baroreceptors and macula densa. The Ca2+ modulation of PTH-induced renin release is consistent with the reported ability of PTH to block calcium channels and relax vascular smooth muscle.
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- Authors
- HELWIG, J.-J., MUSSO, M.-J., JUDES, C., & NICKOLS, G. A.
- Affiliations
- Monsanto (United States) (US)
- ORCID
- NICKOLS, G. ALLEN
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1991-09
- Volume
- 129
- Issue
- 3
- Pages
- 1233-1242
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2168728040
- Cited by
- 42
- References
- 0
- Citations by year
- 2026: 2 · 2023: 2 · 2020: 6 · 2019: 3 · 2015: 2 · 2014: 1 · 2012: 3
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- parathyroid hormone, renin secretion, calcium, ionized calcium, verapamil, Bay K 8644, isoproterenol, calcium channels, baroreceptors, macula densa, vasorelaxation, teriparatide
- MeSH terms
- Animals, 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester, Calcium, Egtazic Acid, Isoproterenol, Kidney, Kinetics, Male, Parathyroid Hormone, Peptide Fragments, Perfusion, Rats, Inbred Strains, Renin, Verapamil, Teriparatide, Rats, In Vitro Techniques
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