THE IN VIVO METABOLISM OF HYDROCORTISONE1
Abstract
The isolation of the C-19 and C-21 saturated urinary metabolites following cortisone administration to man have been described (Dobriner et al., 1950, 1951; Burstein et al., 1953). Recently significant quantities of hydrocortisone (17-hydroxycorticosterone) have become available and the present study reports the isolation from urine, utilizing paper chromatography, of the major α-ketolic and 17-ketosteroid metabolites isolated following the oral administration of hydrocortisone to a man. The isolated steroids were identified by chromatographic mobility on paper, melting points and infra-red spectrometry. The significance of the present study lies in the demonstration of: 1) The in vivo conversion of hydrocortisone into cortisone, 2) the products of reduction of the Δ4-3-keto system, and 3) the pattern of the 11-oxygenated 17-ketosteroid metabolites arising from hydrocortisone.
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5α-THF is a substrate for the side-chain cleavage reaction that produces C19 metabolites of glucocorticoid origin, but this reaction is catalyzed less efficiently than for THF/THE.
“While 5α-THF is also a substrate for this side-chain cleavage reaction, this reaction is catalyzed less efficiently [257].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [257]
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“While 5α-THF is also a substrate for this side-chain cleavage reaction, this reaction is catalyzed less efficiently [257].”
11β-Hydroxysteroid Dehydrogenase
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Metabolic Transformation of Hydrocortisone-4-C14 in Normal Men
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MethodTHE LIVER AND STEROID HORMONE METABOLISM
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- Authors
- BURSTEIN, S., SAVARD, K., & DORFMAN, R. I.
- Affiliations
- Boston University (US)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1953-07
- Volume
- 53
- Issue
- 1
- Pages
- 88-97
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W1970686346
- Cited by
- 48
- References
- 0
- Citations by year
- 2020: 2 · 2019: 1 · 2014: 1
- FWCI
- 10.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Keywords
- cortisol, in vivo metabolism, urinary steroid metabolites, cortisone, 17-ketosteroids, 11-oxygenated steroids, 17-hydroxycorticosterone, oral administration, paper chromatography
- MeSH terms
- Adrenal Cortex, Adrenal Cortex Hormones, Hydrocortisone
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