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An Improved Technique for the Preparation of Isolated Rat Adrenal Cells: A Sensitive, Accurate and Specific Method for the Assay of ACTH
Abstract
Rat adrenal tissue is dispersed with a combination of tryptic digestion and mechanical agitation. Production of corticosterone by the isolated cells in response to a given quantity of ACTH is increased when the calcium concentration of the incubation medium is increased to 3 times that of KRB. Production of corticosterone is enhanced when BSA is added to the medium; production is greater at 0.5% than at 2.0 or 3.0% BSA. Minimum effective dose is 0.1 μU (1.0 pg) of ACTH. The accuracy of the isolated adrenal cell method for assay of ACTH is excellent; λ = 0.01–0.06 compared with λ =0.15–0.25 for other currently employed bioassay methods. In addition, the cells are highly selective; they do not respond to relatively large quantities (107 pg) of insulin, glucagon, oxytocin, vasopressin or angiotensin II. At 107 pg, α-MSH exhibits a weak action corresponding to that exhibited by 10 pg of ACTH. (Endocrinology88: 1063, 1971)
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Cited by
Seelig, S., & Sayers, G. (1973) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(73)90053-2 · Cited by 87
Self-citationIsolation and structure of corticostatin peptides from rabbit fetal and adult lung
Zhu, Q. Z., Hu, J., Mulay, S., Esch, F., Shimasaki, S., & Solomon, S. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.2.592 · Cited by 77
Acute Self-Suppression of Corticosteroidogenesis in Isolated Adrenocortical Cells*
CARSIA, R. V., & MALAMED, S. (1979) · Endocrinology · doi:10.1210/endo-105-4-911 · Cited by 62
Metadata
- Authors
- SAYERS, G., SWALLOW, R. L., & GIORDANO, N. D.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1971-04
- Volume
- 88
- Issue
- 4
- Pages
- 1063-1068
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2060492670
- Cited by
- 261
- References
- 0
- FWCI
- 28.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- SAYERS, G., SWALLOW, R. L., & GIORDANO, N. D. (1971). An Improved Technique for the Preparation of Isolated Rat Adrenal Cells: A Sensitive, Accurate and Specific Method for the Assay of ACTH. Endocrinology. https://doi.org/10.1210/endo-88-4-1063
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