The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Evidence of a corticotropin-releasing hormone pulse generator in the macaque hypothalamus
Abstract
The secretion of hormones from the hypothalamic-pituitary axis is, in general, characterized by an episodic pattern of release. In the adrenal axis, ACTH and cortisol levels in peripheral blood display irregularly pulsatile ultradian patterns that are superimposed on the well characterized circadian rhythm. While it is generally accepted that CRH is released from the hypothalamus in a similar manner, very few studies have actually examined the temporal release of CRH. To examine the temporal release of CRH directly, we have established an in vitro perifusion system using the hemisectioned macaque hypothalamus. Perifusate samples were collected at 10-min intervals for 20 h and assayed for CRH by RIA. In control animals, a very regular, pulsatile pattern of hormone release was present, with a pulse interval of 90 +/- 11 min. Although this interval closely approximates the average pulse interval of ACTH and cortisol in the human, the regular pattern revealed in our study has not been demonstrated previously in the adrenal axis in vivo and suggests that factors outside the hypothalamus play a major role in controlling adrenal hormone levels. When hypothalami were perifused with dexamethasone added to the culture medium, no change in pulsatile activity was detected, indicating that a site outside of the hypothalamus may function as the primary center of feedback inhibition by adrenal glucocorticoids in the central nervous system. Because the very regular pulses of CRH that we observed bear striking similarity to the circhoral pulses of GnRH, we speculate that CRH may play a more subordinate role in regulating the adrenal axis and that other releasing factors and/or feedback effects at the pituitary level may be more important in the generation of the irregularly pulsatile, circadian patterns of ACTH and cortisol seen in peripheral blood.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Subsequent experiments confirmed this model prediction 48, 49, which demonstrated that a previously hypothesised hypothalamic ‘pulse generator’ [50] is not essential to generate ultradian glucocorticoid oscillations.”
From Mathematical Modelling of Endocrine Systems (2019) · cites this paper as [50]
In the citing paper
“The only supportive data come from studies with cultured explants of the macaque hypothalamus (Mershon et al. 1992) and from rat median eminence (Ixart et al. 1991) that show episodic release of CRH.”
From Origin of ultradian pulsatility in the hypothalamic–pituitary–adrenal axis (2010) · cites this paper as Mershon et al. 1992
In the citing paper
“Following Mershon et al. [34] the ultradian oscillations are driven by a CRH pulse generator.”
From Patient specific modeling of the HPA axis related to clinical diagnosis of depression (2017) · cites this paper as [34]
In the citing paper
“In Mershon et al. [34] the existence of a CRH-oscillator in the isolated hypothalamus was suggested based on experiments”
From Patient specific modeling of the HPA axis related to clinical diagnosis of depression (2017) · cites this paper as [34]
In the citing paper
“However, that model is based on the physiological paradigm that ultradian oscillation is due to a corticotropin-releasing hormone pulse generator in macaque hypothalamus (referring to Mershon et al. [34])”
From Patient specific modeling of the HPA axis related to clinical diagnosis of depression (2017) · cites this paper as [34]
Citations
Cites
JACOBSON, L., & SAPOLSKY, R. (1991) · Endocrine Reviews · doi:10.1210/edrv-12-2-118 · Cited by 1,739
The Neuroendocrine Control of the Menstrual Cycle
Knobil, E. (1980) · Elsevier eBooks · doi:10.1016/b978-0-12-571136-4.50008-5 · Cited by 1,285
Corticosteroid inhibition of acth secretion
KELLER-WOOD, M. E., & DALLMAN, M. F. (1984) · Endocrine Reviews · doi:10.1210/edrv-5-1-1 · Cited by 1,219
The neuronal mineralocorticoid eeceptor as a mediator of glucocorticoid response
Arriza, J. L., Simerly, R. B., Swanson, L. W., & Evans, R. M. (1988) · Neuron · doi:10.1016/0896-6273(88)90136-5 · Cited by 591
Absence of oestradiol concentration in cell nuclei of LHRH-immunoreactive neurones
Shivers, B. D., Harlan, R. E., Morrell, J. I., & Pfaff, D. W. (1983) · Nature · doi:10.1038/304345a0 · Cited by 531
Mediation by Corticotropin Releasing Factor (CRF) of Adenohypophysial Hormone Secretion
RIVIER, C. L., & Plotsky, P. M. (1986) · Annual Review of Physiology · doi:10.1146/annurev.ph.48.030186.002355 · Cited by 444
RIVIER, C. L., & Vale, W. (1983) · Endocrinology · doi:10.1210/endo-113-3-939 · Cited by 407
SANTORO, N., FILICORI, M., & CROWLEY, W. F. (1986) · Endocrine Reviews · doi:10.1210/edrv-7-1-11 · Cited by 255
SMITH, M. A., & Vale, W. (1981) · Endocrinology · doi:10.1210/endo-108-3-752 · Cited by 175
Reppert, S. M., Mj, P., Ungerleider, L. G., Mishkin, M., Tamarkin, L., Orloff, D. G., Hj, H., & Klein, D. C. (1981) · Journal of Neuroscience · doi:10.1523/jneurosci.01-12-01414.1981 · Cited by 148
Cited by
Sorted by citations
The crucial role of pulsatile activity of the HPA axis for continuous dynamic equilibration
Lightman, S. L., & Conway-Campbell, B. L. (2010) · Nature Reviews Neuroscience · doi:10.1038/nrn2914 · Cited by 390
Spiga, F., Walker, J. J., Terry, J. R., & Lightman, S. L. (2014) · Comprehensive Physiology · doi:10.1002/cphy.c140003 · Cited by 267
Origin of ultradian pulsatility in the hypothalamic–pituitary–adrenal axis
Walker, J. J., Terry, J. R., & Lightman, S. L. (2010) · Proceedings of the Royal Society B: Biological Sciences · doi:10.1098/rspb.2009.2148 · Cited by 239
“The only supportive data come from studies with cultured explants of the macaque hypothalamus (Mershon et al. 1992) and from rat median eminence (Ixart et al.”
BackgroundMotivations and Methods for Analyzing Pulsatile Hormone Secretion
Veldhuis, J. D., Keenan, D. M., & Pincus, S. M. (2008) · Endocrine Reviews · doi:10.1210/er.2008-0005 · Cited by 223
Windle, R. J., Wood, S. A., Lightman, S. L., & Ingram, C. D. (1998) · Endocrinology · doi:10.1210/endo.139.10.6238 · Cited by 201
Metadata
- Authors
- Mershon, J. L., Sehlhorst, C. S., Rebar, R. W., & Liu, J. H.
- Affiliations
- University of Cincinnati Medical Center (US)
- ORCID
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1992-05
- Volume
- 130
- Issue
- 5
- Pages
- 2991-2996
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2034889240
- Cited by
- 70
- References
- 18
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 6 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 2
- FWCI
- 0.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- corticotropin-releasing factor, pulsatile hormone secretion, hypothalamus, macaque, hypothalamic-pituitary-adrenal axis, ACTH, cortisol, ultradian rhythms, circadian rhythm, glucocorticoid feedback inhibition, gonadotropin-releasing hormone, perifusion
- MeSH terms
- Animals, Corticotropin-Releasing Hormone, Dexamethasone, Female, Hypothalamus, Kinetics, Macaca fascicularis, Macaca mulatta, Male, Perfusion, Radioimmunoassay, Time Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
