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Cloning and characterization of human urocortin.
Abstract
Urocortin, a new member of the CRF peptide family which also includes urotensin I and sauvagine, was recently cloned from the rat midbrain. The synthetic replicate of urocortin was found to bind with high affinity to type 1 and type 2 CRF receptors and, based upon its anatomic localization within the brain, was proposed to be a natural ligand for the type 2 CRF receptors. Using a genomic library, we have cloned the human counterpart of rat urocortin and localized it to human chromosome 2. Human and rat urocortin share 95% identity within the mature peptide region. Synthetic human urocortin binds with high affinity to CRF receptor types 1, 2 alpha, and 2 beta, stimulates cAMP accumulation from cells stably transfected with these receptors, and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. In addition, the CRF-binding protein binds human urocortin with high affinity and can prevent urocortin-stimulated ACTH secretion in vitro. The inhibitory effect of the CRF-binding protein on human urocortin can be blocked by biologically inactive CRF fragments, such as CRF(9-33).
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Cited by
The role of corticotropin-releasing factor in depression and anxiety disorders
Arborelius, L., Owens, M., Plotsky, P., & Nemeroff, C. (1999) · Journal of Endocrinology · doi:10.1677/joe.0.1600001 · Cited by 1,378
Slominski, A., Wortsman, J., Luger, T., Paus, R., & Solomon, S. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.3.979 · Cited by 776
Radulovic, J., Rühmann, A., Liepold, T., & Spiess, J. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-12-05016.1999 · Cited by 402
Coulouarn, Y., Lihrmann, I., Jegou, S., Anouar, Y., Tostivint, H., Beauvillain, J. C., Conlon, J. M., Bern, H. A., & Vaudry, H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15803 · Cited by 395
The CRF system, stress, depression and anxiety—insights from human genetic studies
Binder, E. B., & Nemeroff, C. B. (2010) · Molecular Psychiatry · doi:10.1038/mp.2009.141 · Cited by 379
Metadata
- Authors
- Donaldson, C. J., Sutton, S. W., Perrin, M. H., Corrigan, A. Z., Lewis, K. A., Rivier, J. E., Vaughan, J. M., & Vale, W. W.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1996-05
- Volume
- 137
- Issue
- 5
- Pages
- 2167-2170
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2054550208
- Cited by
- 372
- References
- 0
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- Donaldson, C. J., Sutton, S. W., Perrin, M. H., Corrigan, A. Z., Lewis, K. A., Rivier, J. E., Vaughan, J. M., & Vale, W. W. (1996). Cloning and characterization of human urocortin. Endocrinology. https://doi.org/10.1210/endo.137.5.8612563
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