CoChat
Sign Up
Cited by 437Regulation of Appetite and ObesityHypothalamic control of reproductive hormonesAdipokines, Inflammation, and Metabolic Diseases

The DOI matches its Crossref record.

Regulation of Hypothalamic Proopiomelanocortin mRNA by Leptin in ob/ob Mice

Thornton, Janice E., Cheung, Clement C., Clifton, Donald K., Steiner, Robert A.·1997·Endocrinology
DOI 10.1210/endo.138.11.5651
Open this paper in CoChat — free account

Abstract

The hormone leptin acts on the brain to regulate feeding, metabolism, and reproduction; however, its cellular targets and molecular mechanisms of action remain to be fully elucidated. The melanocortins, which are derived from the precursor proopiomelanocortin (POMC), are also implicated in the physiological regulation of body weight. POMC-containing neurons express the leptin receptor, and thus it is conceivable that the POMC gene itself may be part of the signaling pathway involved in leptin's action on the brain. Using in situ hybridization and computerized image analysis, we tested the hypothesis that the POMC gene is a target for regulation by leptin by comparing cellular levels of POMC mRNA in the hypothalamus among groups of leptin-deficient (ob/ob) mice, leptin-treated ob/ob mice, and wild-type controls. POMC mRNA levels were significantly reduced throughout the arcuate nucleus in vehicle-treated ob/ob mice relative to wild-type controls, whereas POMC mRNA levels in leptin-treated ob/ob mice were indistinguishable from wild-type controls. These observations suggest that one or more products of POMC serve as an integrative link between leptin and the central mechanisms governing body weight regulation and reproduction.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1210/endo.138.11.5651

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

The publisher hasn’t deposited this paper’s reference list.

Research works printed but not linked: 3

  1. [11]11. Mercer JG, Hoggard N, Williams LM, Lawrence CB, Hannah LT, Trayhurn P 1996 Localization of leptin receptor mRNA and the long form splice variant (Ob-Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization. FEBS Letters 387:113-116.
  2. [22]22. Cheung CC, Clifton DK, Steiner RA in press Proopiomelanocortin neurons are direct targets for leptin in the hypothalamus. Endocrinology
  3. [29]29. Franklin KBJ, Paxinos G 1997 The mouse brain in stereotaxic coordinates, Ed., Academic Press,

Cited by

Sorted by citations

Items 401–424 of 424

Related

See all

Metadata

Authors
Thornton, J. E., Cheung, C. C., Clifton, D. K., & Steiner, R. A.
Affiliations
University of Washington (US)
Corresponding author
Clifton, Donald K.
ORCID
Steiner, Robert A.
Venue
Endocrinology
Publisher
The Endocrine Society
Published
1997-11-01
Volume
138
Issue
11
Pages
5063-5066
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/endo.138.11.5651
OpenAlex
W2064342369
Cited by
437
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 7 · 2018: 3 · 2017: 5 · 2016: 7 · 2015: 12 · 2014: 9 · 2013: 12 · 2012: 9
FWCI
12.23
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
  • ResearchWorks at the University of Washington (University of Washington) (submittedVersion)
Funders
Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01 HD 27142, P50HD012629, R01HD027142, P50-HD-12629)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
Keywords
leptin, proopiomelanocortin, POMC mRNA, hypothalamus, arcuate nucleus, ob/ob mice, leptin receptor, melanocortin peptides, body weight regulation, reproduction, gene expression regulation
MeSH terms
Animals, Cell Count, Eating, Hypothalamus, Male, Mice, Mutant Strains, Neurons, Obesity, Pro-Opiomelanocortin, Proteins, RNA, Messenger, Leptin, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review